Diffusion Plate Assay for Chloramphenicol and Aureomycin 1

An alternative colorimetric method for the determination of chloramphenicol

“…Various methods are available for the assay of the compound in bulk drug, in dosage forms and in biological fluids [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] . These include colorimetric [5][6][7][8] , microbiological [9][10][11] , radio-immunoassay 12,13 , high performance liquid chromatography [14][15][16][17] , gas liquid chromatography 18 , spectrophotometric 19,20 , and polarographic 21 methods.…”

Biochemistry of Antibiotics

“…This property has been applied as the basis for a chemical determination of aureomycin (143). Microbiological assay methods for aureomycin also have been reported (144,145,146).…”

Rapid Microbiological Assay for Chloramphenicol and Tetracyclines

“…In the past, serum chloramphenicol levels have been determined by tube dilution (8), turbidimetric (9,20), and by agar diffusion techniques (12,16). These microbiological assays have not attained widespread usage because of their technical complexity (9,20), low sensitivity (12), prolonged incubation times, and interference by concurrently present antimicrobial agents.…”

“…These microbiological assays have not attained widespread usage because of their technical complexity (9,20), low sensitivity (12), prolonged incubation times, and interference by concurrently present antimicrobial agents. The colorimetric (1, 6) and gas-liquid chromatographic methods (13) require more technical sophistication than is generally available in a hospital laboratory.…”