Diffusion of polystyrene in gels

Bansil, Rama; Pajevic, Sinisa; Koňák, Čestmı́r

doi:10.1021/ma00215a028

Cited by 22 publications

(29 citation statements)

References 1 publication

(1 reference statement)

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“…4 as a function of the number of bases. The variation is compatible with the prediction of reptation of a Gaussian chain (slope = -2) [30] although a direct fit would be closer to the -1.8 value observed by Bansil et al [31] for polystyrene in polymethylmethacrylate gels and by D. Pernodet et al…”

Section: Diffusion Coefficientssupporting

confidence: 89%

Migration of single‐stranded DNA in polyacrylamide gels during electrophoresis

et al. 1998

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By using fluorescence recovery after photobleaching (FRAP) and electric birefringence, the migration of single-stranded DNA in polyacrylamide gels and orientation as a response to an electric pulse were investigated. Electrophoretic mobility is in good agreement with the model of biased reptation including fluctuations. The determination of the electrophoretic mobility in solution, mu0, allows an estimation of the gel pore diameter seen by the molecule. As previously observed for double-stranded DNA, the electric birefringence results from two processes: the alignment of the molecule along the electric field and the elongation of the primitive path in the gel, for long single-stranded DNA (>2000 bases). The combination of results obtained with the two techniques allows us to propose experimental conditions to improve the separation of single-stranded DNA with pulsed field techniques.

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Section: Diffusion Coefficientssupporting

confidence: 89%

Migration of single‐stranded DNA in polyacrylamide gels during electrophoresis

et al. 1998

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“…3, three of the DNA samples (9416 bp, 23,132 bp, and 48,502 bp) seem to fall in the reptation regime. The actual fit of the data for these three chain lengths gives an experimental scaling exponent of Ϫ1.55, which is close to the published measurements given by Bansil et al (1990), Tinland et al, (1998), and Pluen et al (1998) for different types of polyelectrolyte chains within the reptation regime. Based on the hypothesis that the behavior of these three DNA chains is in the reptation regime, we proceeded to apply the corresponding polymer dynamics equations to each DNA to estimate the effective pore size of 2% agarose gel in 0.1 M PBS.…”

Section: Diffusion Of Dna Moleculessupporting

confidence: 86%

Diffusion of Macromolecules in Agarose Gels: Comparison of Linear and Globular Configurations

Pluen

Netti

Jain

et al. 1999

Biophysical Journal

522

475

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The diffusion coefficients (D) of different types of macromolecules (proteins, dextrans, polymer beads, and DNA) were measured by fluorescence recovery after photobleaching (FRAP) both in solution and in 2% agarose gels to compare transport properties of these macromolecules. Diffusion measurements were conducted with concentrations low enough to avoid macromolecular interactions. For gel measurements, diffusion data were fitted according to different theories: polymer chains and spherical macromolecules were analyzed separately. As chain length increases, diffusion coefficients of DNA show a clear shift from a Rouse-like behavior (DG congruent with N0-0.5) to a reptational behavior (DG congruent with N0-2.0). The pore size, a, of a 2% agarose gel cast in a 0.1 M PBS solution was estimated. Diffusion coefficients of the proteins and the polymer beads were analyzed with the Ogston model and the effective medium model permitting the estimation of an agarose gel fiber radius and hydraulic permeability of the gels. Not only did flexible macromolecules exhibit greater mobility in the gel than did comparable-size rigid spherical particles, they also proved to be a more useful probe of available space between fibers.

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“…In many investigations, the slope was determined to be between −2 and −3 14, 16–21. Only few investigations measured weaker dependences on M 22, 23. Muthukumar tried to explain these findings by the structure of the network 24.…”

Section: Resultsmentioning

confidence: 99%

“…In many investigations, the slope was determined to be between À2 and À3. [14,[16][17][18][19][20][21] Only few investigations measured weaker dependences on M. [22,23] Muthukumar tried to explain these findings by the structure of the network. [24] He carried out computer simulations on probe dynamics in homogeneous and heterogeneous network structures and found that a stronger dependence of the diffusion behavior on the molecular weight of the polymer tracer (D $ M…”

Section: à2mentioning

confidence: 99%

Dynamics of Labeled Linear Polystyrenes in Semi‐Dilute Polystyrene Matrices in the Uncross‐Linked and Cross‐Linked States

Susoff

2010

Macromolecular Symposia

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Summary: Linear polystyrene chains that were labeled with a fluorescent dye were enclosed in a semidilute matrix of polystyrene in toluene. The matrix polymers used were suitably functionalized such that they could be photocross-linked in a stepwise and controlled manner without attachment of the tracer chains. The diffusion coefficient of the tracer chains was determined by fluorescence recovery after photobleaching while the state of the matrix was changing from a semidilute solution to a covalently cross-linked gel. The progress of the photocross-linking reaction was macroscopically monitored by rheology. The tracer chains were polystyrenes with molecular weights ranging from 50 000 to 2 000 000 g mol À1 and low polydispersity.They were labeled via polymer analogous reactions with the fluorescent dye 6-(7-nitrobenzfurazan-4-ylamino) hexanoic acid. The matrix polymers covered a similar molecular weight range. The results of these measurements show that the dependence of the diffusion coefficient on the tracer molecular weight in the gel can be described by a scaling law in the sense of the reptation theory whereas distinct deviations of this scaling behavior appear in the sol state. The ratio of the matrix molecular weight and tracer molecular weight in the initial sol state significantly influences the change of the probe dynamics during the sol-gel transition.

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Diffusion of polystyrene in gels

Cited by 22 publications

References 1 publication

Migration of single‐stranded DNA in polyacrylamide gels during electrophoresis

Migration of single‐stranded DNA in polyacrylamide gels during electrophoresis

Diffusion of Macromolecules in Agarose Gels: Comparison of Linear and Globular Configurations

Dynamics of Labeled Linear Polystyrenes in Semi‐Dilute Polystyrene Matrices in the Uncross‐Linked and Cross‐Linked States

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