Diffusion of polyphenols from alginate, alginate/chitosan, and alginate/inulin particles

Pravilović, Radoslava; Balanč, Bojana D.; Djordjević, Vladan; Bošković‐Vragolović, Nevenka; Bugarski, Branko; Pjanović, Rada

doi:10.1111/jfpe.13043

Cited by 9 publications

(5 citation statements)

References 31 publications

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“…Consequently, compound 64 was tentatively assigned to be an acacetin-rhamnoglucoside isomer. The molecular ion at m/z 767.52 was noted as an acacetin derivative (72). According to the literature data, the peak at t R 12.24 min that resulted from diosmetin-7-O-glucuronide-3 ′ -O-pentoside ( 67 The identified phenolic compounds in the black locust flower extract are in accordance with previously reported data [40].…”

Section: Uhplc-ms Analysissupporting

confidence: 86%

“…The formed complex does not allow easy penetration of fluid inside the alginate–chitosan microparticles. Pravilović et al [ 72 ] also showed a lower SD of alginate–chitosan microparticles encapsulated with thyme extracts compared to the alginate microparticles. The results of swelling studies indicated that SD increased as the pH value of the medium increased.…”

Section: Resultsmentioning

confidence: 99%

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Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Boškov,

Savić,

Grozdanić Stanisavljević

et al. 2024

Polymers

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Black locust flower extract contains various polyphenols and their glucosides contribute to the potential health benefits. After intake of these bioactive compounds and passage through the gastrointestinal tract, their degradation can occur and lead to a loss of biological activity. To overcome this problem, the bioactive compounds should be protected from environmental conditions. This study aimed to encapsulate the black flower extract in the microparticles based on biodegradable polysaccharides, alginate, and chitosan. In the extract, the total antioxidant content was found to be 3.18 ± 0.01 g gallic acid equivalent per 100 g of dry weight. Also, the presence of lipids (16), phenolics (27), organic acids (4), L-aspartic acid derivative, questinol, gibberellic acid, sterol, and saponins (2) was confirmed using the UHPLC–ESI–MS analysis. In vitro assays showed that the extract has weak anti-α-glucosidase activity and moderate antioxidant and cytotoxic activity against the HeLa cell line. The extrusion method with secondary air flow enabled the preparation of microparticles (about 270 μm) encapsulated with extract. An encapsulation efficiency of over 92% was achieved in the alginate and alginate–chitosan microparticles. The swelling study confirmed a lower permeability of alginate–chitosan microparticles compared with alginate microparticles. For both types of microparticles, the release profile of antioxidants in the simulated gastrointestinal fluids at 37 °C followed the Korsmeyer–Peppas model. A lower diffusion coefficient than 0.5 indicated the simple Fick diffusion of antioxidants. The alginate–chitosan microparticles enabled a more sustained release of antioxidants from extract compared to the alginate microparticles. The obtained results indicated an improvement in the antioxidant activity of bioactive compounds from the extract and their protection from degradation in the simulated gastric conditions via encapsulation in the polymer matrixes. Alginate–chitosan showed slightly slower cumulative antioxidant release from microparticles and better antioxidant activity of the extract compared to the alginate system. According to these results, alginate–chitosan microparticles are more suitable for further application in the encapsulation of black locust flower extract. Also, the proposed polymer matrix as a drug delivery system is safe for human use due to its biodegradability and non-toxicity.

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Section: Uhplc-ms Analysissupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Boškov,

Savić,

Grozdanić Stanisavljević

et al. 2024

Polymers

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“…In contrast, inhomogeneous hydrogel structures obtained from external cross-linking has smaller gel networks on the alginate surface with pore sizes of 12–16 nm . Note that the pore size also depends on the type of cross-linking cations, alginate M/G ratios, and the concentration of alginate. − For instance, Thu et al demonstrated that Bovine serum albumin (BSA) diffused out of alginate hydrogels at a higher rate in hydrogels with lower concentration of alginate . It is known that the porosity decreases with increasing amounts of G-blocks, which interact with cations to form cross-links.…”

Section: Properties Of Alginate Beneficial For 3d Cell Culturementioning

confidence: 99%

Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture

Kang

Lee

Huh

et al. 2020

ACS Biomater. Sci. Eng.

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Advances in microscale 3D cell culture systems have helped to elucidate cellular physiology, understand mechanisms of stem cell differentiation, produce pathophysiological models, and reveal important cell–cell and cell–matrix interactions. An important consideration for such studies is the choice of material for encapsulating cells and associated extracellular matrix (ECM). This Review focuses on the use of alginate hydrogels, which are versatile owing to their simple gelation process following an ionic cross-linking mechanism in situ, with no need for procedures that can be potentially toxic to cells, such as heating, the use of solvents, and UV exposure. This Review aims to give some perspectives, particularly to researchers who typically work more with poly(dimethylsiloxane) (PDMS), on the use of alginate as an alternative material to construct microphysiological cell culture systems. More specifically, this Review describes how physicochemical characteristics of alginate hydrogels can be tuned with regards to their biocompatibility, porosity, mechanical strength, ligand presentation, and biodegradability. A number of cell culture applications are also described, and these are subcategorized according to whether the alginate material is used to homogeneously embed cells, to micropattern multiple cellular microenvironments, or to provide an outer shell that creates a space in the core for cells and other ECM components. The Review ends with perspectives on future challenges and opportunities for 3D cell culture applications.

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“…Assuming a simplified system where polyphenols are not bound with a matrix, the antioxidant migration should only be dependent on simple molecular diffusion, exhibiting a burst release as all polyphenols near the surface diffuse out rapidly, followed by slower diffusion of internal polyphenols out of the bulk. However, in addition to complex polymer dynamics, the previously described chemical properties of polyphenols invoke diverse interactions with a wide variety of polymers, further complicating the delivery of active antioxidants …”

Section: Integration Of Polyphenols With Polymeric Materialsmentioning

confidence: 99%

“…However, in addition to complex polymer dynamics, the previously described chemical properties of polyphenols invoke diverse interactions with a wide variety of polymers, further complicating the delivery of active antioxidants. 127 An understanding of the physicochemical properties of both the polymer and polyphenol are necessary for the strategic design of migratory films. Depending on the intended environment for the film, the selection of the polymer should consider swelling/deswelling, which could accelerate/inhibit antioxidant release, and chemistry of functional groups, which could influence the formation of intermolecular bonds.…”

Section: Integration Of Polyphenols With Polymeric Materialsmentioning

confidence: 99%

Integrating Antioxidant Functionality into Polymer Materials: Fundamentals, Strategies, and Applications

Brito

Hlushko

Abbott

et al. 2021

ACS Appl. Mater. Interfaces

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While antioxidants are widely known as natural components of healthy food and drinks or as additives to commercial polymer materials to prevent their degradation, recent years have seen increasing interest in enhancing the antioxidant functionality of newly developed polymer materials and coatings. This paper provides a critical overview and comparative analysis of multiple ways of integrating antioxidants within diverse polymer materials, including bulk films, electrospun fibers, and self-assembled coatings. Polyphenolic antioxidant moieties with varied molecular architecture are in the focus of this Review, because of their abundance, nontoxic nature, and potent antioxidant activity. Polymer materials with integrated polyphenolic functionality offer opportunities and challenges that span from the fundamentals to their applications. In addition to the traditional blending of antioxidants with polymer materials, developments in surface grafting and assembly via noncovalent interaction for controlling localization versus migration of antioxidant molecules are discussed. The versatile chemistry of polyphenolic antioxidants offers numerous possibilities for programmed inclusion of these molecules in polymer materials using not only van der Waals interactions or covalent tethering to polymers, but also via their hydrogen-bonding assembly with neutral molecules. An understanding and rational use of interactions of polyphenol moieties with surrounding molecules can enable precise control of concentration and retention versus delivery rate of antioxidants in polymer materials that are critical in food packaging, biomedical, and environmental applications.

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Diffusion of polyphenols from alginate, alginate/chitosan, and alginate/inulin particles

Cited by 9 publications

References 31 publications

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles

Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture

Integrating Antioxidant Functionality into Polymer Materials: Fundamentals, Strategies, and Applications

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