Diffusion in Soft Polymer Systems as Approached by Field-Gradient NMR

Yamane, Yuji; Kanesaka, Shigeru; Kim, S.; Kamiguchi, Kazuhiro; Matsui, Masanao; Kuroki, Shigeki; Ando, Isao

doi:10.1016/s0066-4103(05)58002-5

Cited by 7 publications

(6 citation statements)

References 177 publications

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“…The self‐diffusion of macromolecules in solutions is influenced by a number of factors, such as macromolecular collisions, self‐association and network formation , that may lead to decreasing diffusion coefficients in a time‐dependent manner. Each of these processes could take place in the HA‐water system.…”

Section: Resultsmentioning

confidence: 99%

“…The apparent fraction of the DD components for HA increases with rising temperature because 1 H T 2 and T 1 values are temperature dependent. The self-diffusion of macromolecules in solutions is influenced by a number of factors, such as macromolecular collisions, [13] self-association [14] and network formation [15] , that may lead to decreasing diffusion coefficients in a time-dependent manner. Each of these processes could take place in the HA-water system.…”

Section: Resultsmentioning

confidence: 99%

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Self‐diffusion in a hyaluronic acid–albumin–water system as studied by NMR

Филиппов

Artamonova

Rudakova

et al. 2012

Magnetic Reson in Chemistry

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Hyaluronic acid (HA) is an anionic biopolymer that is present in many tissues and can be involved in cancerous neoformations. HA can form complexes with proteins (particularly, serum albumin) in the body. However, HA structures and processes involving HA have not been extensively studied by NMR because the molecule's rigid structure makes these studies problematic. In the current work, self-diffusion of HA and bovine serum albumin (BSA), and water in solutions was measured by (1)H pulsed field gradient NMR (PFG NMR) with a focus on the HA-BSA-D2O systems at various concentrations of BSA and HA. It was shown that in the presence of even a small amount of HA, the self-diffusion coefficient (SDC) of BSA decreases. To explain this fact, three hypotheses were proposed and analyzed. The first one was based on the effect of slowing down of water mobility in the presence of HA. The second hypothesis suggested an effect of mechanical collisions of BSA with HA molecules. The third hypothesized that BSA and HA molecules form a complex where BSA molecules reduced in mobility. It was shown that the third mechanism is the most likely. The state of the BSA molecules in the BSA-HA-D2O system corresponds to a 'fast exchange' condition from the NMR point of view: BSA molecules reside in the 'free' and 'bound' (with HA) states for much shorter time than the diffusion time of the PFG NMR experiment, 7 ms. The fractions of 'bound' BSA molecules in the BSA-HA complex were estimated.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Self‐diffusion in a hyaluronic acid–albumin–water system as studied by NMR

Филиппов

Artamonova

Rudakova

et al. 2012

Magnetic Reson in Chemistry

View full text Add to dashboard Cite

show abstract

“…In the diffusion coefficient (D) measurements, two kinds of field-gradient NMR methods such as PFGSE method [38][39][40][41][42][43][44][45][46] and PFGStE method [44,[47][48][49], are generally used to determine the diffusion coefficients for polymer systems. These methods have been successfully used in our previous works on diffusional behavior in polymer gel systems [46,47,49], polymer liquid crystals [11][12][13] and n-paraffins [50][51][52][53].…”

mentioning

confidence: 99%

“…These methods have been successfully used in our previous works on diffusional behavior in polymer gel systems [46,47,49], polymer liquid crystals [11][12][13] and n-paraffins [50][51][52][53]. In this work the PFGStE method has been used.…”

mentioning

confidence: 99%