Diffusion-controlled Mediated Electron Transfer-type Bioelectrocatalysis Using Microband Electrodes as Ultimate Amperometric Glucose Sensors

Matsui, Yukina; Hamamoto, Katsumi; Kitazumi, Yuki; Shirai, Osamu; Kano, Kenji

doi:10.2116/analsci.33.845

Cited by 31 publications

(20 citation statements)

References 40 publications

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“…Oxygen-insensitive flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) has been given much attention recently and there has been an increasing numbers of related reports on biosensors [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] and biofuel cells. 13,14,[16][17][18] Recently, the structure of FAD-GDH from Aspergillus flavus was unveiled and it was found that an FAD cofactor is buried deeply (∼1.4 nm) below the protein surface.…”

Section: Introductionmentioning

confidence: 99%

“…MET is the utilization of electron transfer mediators such as potassium hexacyanoferrate (K3[Fe(CN)6]), 5 phenazine methosulfate, 3,7 osmium complexes, [8][9][10] phenothiazin, 13 and naphthoquiobne. 15 Small and mobile mediators ferry electrons from FAD, which originates from an enzyme-catalytic reaction, to the electron collector ( Fig. 1(A)).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Direct and Mediated Electron Transfer in Electrodes with Novel Fungal Flavin Adenine Dinucleotide Glucose Dehydrogenase

et al. 2018

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Direct and mediated electron transfer (DET and MET) in enzyme electrodes with a novel flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) from fungi are compared for the first time. DET is achieved by placing a single-walled carbon nanotube (CNT) between GDH and a flat gold electrode where the CNT is close to FAD within the distance for DET. MET is induced by using a free electron transfer mediator, potassium hexacyanoferrate, and shuttles electrons from FAD to the gold electrode. Cyclic voltammetry shows that the onset potential for glucose response current in DET is smaller than in MET, and that the distinct redox current peak pairs in MET are observed whereas no peaks are found in DET. The chronoamperometry with respect to a glucose biosensor shows that (i) the response in DET is more rapid than in MET; (ii) the current at more than +0.45V in DET is larger than the current at the current-peak potential in MET; (iii) a DET electrode covers the glucose concentration range for clinical requirements and is not susceptible to interfering agents at +0.45 V; and (iv) a DET electrode with the novel fungal FAD-GDH does not affect sensing accuracy in the presence of up to 5 mM xylose, while it often shows a similar response level to glucose with other conventionally used fungus-derived FAD-GDHs. It is concluded that our DET system overcomes the disadvantage of MET.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison of Direct and Mediated Electron Transfer in Electrodes with Novel Fungal Flavin Adenine Dinucleotide Glucose Dehydrogenase

et al. 2018

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“…The location of the reaction plane depends on the enzyme kinetics and c S (the concentrations of S) and the current is determined by the steady-state mass transfer of S to the semispherical reaction plane near the microdisk electrode. 79 The current can be intensified by using an ultrathin ring film electrode 80 or a micro-band electrode 81 at which pseudo-steady-state currents can obtained as in the general case expressed as follows: 82 ið¸Þ…”

Section: Biosensorsmentioning

confidence: 99%

Fundamentals and Applications of Redox Enzyme-functionalized Electrode Reactions

Kano

2019

Electrochemistry

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Electrochemical coupling of redox enzyme reactions, called bioelectrocatalysis, has been attracting great attention over the last four decades. It has become an important technology that can be applied to a wide range of bioelectrochemical devices including biosensors, biofuel cells, and bioreactors. This article presents an overview of the basic concepts of steady-state catalytic waves of mediated-and direct electron transfer (DET)-type bioelectrocatalysis. Several equations that can be used for the analysis of steady-state waves are introduced. The analysis may provide important thermodynamic and kinetic parameters that can be used not only for performance evaluation of the devices but also for fundamental research on the enzymes. Important progress made on how to tune electrode surfaces and enzymes for DET-type reactions are presented. Applications to bioelectrochemical devices are also summarized with emphasis on the achievements recorded in our research group.

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“…If one can use biosensors without calibration, it is easy to monitor the concentration of target analytes. In this sense, microelectrode detection was introduced to develop amperometric biosensors [ 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. Nonlinear diffusion of the substrate at microelectrodes causes diffusion-controlled steady-state currents.…”

Section: Introductionmentioning

confidence: 99%

Development Perspective of Bioelectrocatalysis-Based Biosensors

Adachi

Kitazumi

Shirai

et al. 2020

Sensors

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Bioelectrocatalysis provides the intrinsic catalytic functions of redox enzymes to nonspecific electrode reactions and is the most important and basic concept for electrochemical biosensors. This review starts by describing fundamental characteristics of bioelectrocatalytic reactions in mediated and direct electron transfer types from a theoretical viewpoint and summarizes amperometric biosensors based on multi-enzymatic cascades and for multianalyte detection. The review also introduces prospective aspects of two new concepts of biosensors: mass-transfer-controlled (pseudo)steady-state amperometry at microelectrodes with enhanced enzymatic activity without calibration curves and potentiometric coulometry at enzyme/mediator-immobilized biosensors for absolute determination.

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Diffusion-controlled Mediated Electron Transfer-type Bioelectrocatalysis Using Microband Electrodes as Ultimate Amperometric Glucose Sensors

Cited by 31 publications

References 40 publications

Comparison of Direct and Mediated Electron Transfer in Electrodes with Novel Fungal Flavin Adenine Dinucleotide Glucose Dehydrogenase

Comparison of Direct and Mediated Electron Transfer in Electrodes with Novel Fungal Flavin Adenine Dinucleotide Glucose Dehydrogenase

Fundamentals and Applications of Redox Enzyme-functionalized Electrode Reactions

Development Perspective of Bioelectrocatalysis-Based Biosensors

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