Diffusion coefficient and capacity factor in capillary electrokinetic chromatography with replaceable charged polymeric pseudophase

Maichel, Beate; Gaš, Bohuslav; Kenndler, Ernst

doi:10.1002/(sici)1522-2683(20000501)21:8<1505::aid-elps1505>3.0.co;2-e

Cited by 11 publications

(1 citation statement)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The diffusion coefficients of solutes in pDADMA solutions were measured and modelled [23]. Diffusion was modeled given two states with different diffusion coefficients, one when the solute is in the free solution between the polymer chains and one when the solute is interacting with the polymer chains.…”

Section: Cationic Polymersmentioning

confidence: 99%

Recent progress in the development, characterization and application of polymeric pseudophases for electrokinetic chromatography

Palmer

2002

Electrophoresis

View full text Add to dashboard Cite

This review article details the development, characterization and application of polymeric materials as pseudostationary phases for electrokinetic chromatography over the past two years. Recent developments in cationic polymers and anionic siloxane, acrylamide and polymerized surfactants (micelle polymers) are reviewed. Also reviewed is recent progress in the development and characterization of chiral polymeric phases for chiral separations by electrokinetic chromatography, and application of a polymeric pseudophase with electrospray ionization mass spectrometric detection.

show abstract

Section: Cationic Polymersmentioning

confidence: 99%

Recent progress in the development, characterization and application of polymeric pseudophases for electrokinetic chromatography

Palmer

2002

Electrophoresis

View full text Add to dashboard Cite

show abstract

Recent innovation in capillary electrokinetic chromatography with replaceable charged pseudostationary phases or additives

Maichel¹,

Kenndler²

2000

Electrophoresis

Self Cite

View full text Add to dashboard Cite

Recent developments of separation of neutral analytes in capillary systems with the mobile phase driven by the electroosmotic flow (EOF) and charged additives acting as a pseudostationary phase are reviewed. As pseudostationary phases a number of additives are used. Soluble polymers, either anionic or cationic, were applied as alternatives to micelles. Monomeric charged additives are also intended to form associates with the analytes, leading to selective retention and separation in a similar way as the polymeric pseudostationary phases. Dendrimers, spherical macromolecules with highly branched chains and charged terminal groups, are successfully applied for the separation of lipophilic analytes. Polymers with covalently stabilized structures are introduced in the form of permanent micelles and are therefore insensitive to the mobile phase composition, enlarging the applicability of micellar electrokinetic capillary chromatography (MEKC).

show abstract

Modelling and optimization of the electrokinetic chromatographic separation of mixtures of organic anions and cations using poly(diallydimethyl- ammonium chloride) and hexanesulfonate as mixed pseudostationary phases

et al. 2002

View full text Add to dashboard Cite

The separation of a series of aromatic carboxylic acids, sulfonates and opiates using electrokinetic chromatography employing a mixture of the soluble cationic polymer poly(diallydimethylammonium chloride) (PDDAC) and the amphiphilic anion hexanesulfonate as pseudostationary phases is described. In this system, the PDDAC pseudostationary phase interacts with the anionic analytes, whereas the hexanesulfonate pseudostationary phase interacts with the cationic analytes. A migration model has been derived which takes into account the ion-exchange (IE) interactions between the anions and the cationic PDDAC as well as the ion-pair (IP) interactions between the opiates and the hexanesulfonate. A further interaction between the combined PDDAC-hexanesulfonate complex and the more hydrophobic analytes is also evident and is accounted for in the model. Constants obtained by applying the model agreed well with the expected trends in IE affinities of the anions for PDDAC and also corresponded with the hydrophobic natures of the analytes. Optimization of the PDDAC and hexanesulfonate concentrations was performed using the normalized resolution product and minimum resolution product criteria. The minimum resolution product criterion proved to be most successful. An advantage of the described system is the improvement in peak shapes obtained after addition of hexanesulfonate to the electrolyte, resulting in increased plate numbers and better resolution. The system was very robust with mobilities varying by less than 2% over a period of days and on using different capillaries.

show abstract

Diffusion coefficient and capacity factor in capillary electrokinetic chromatography with replaceable charged polymeric pseudophase

Cited by 11 publications

References 32 publications

Recent progress in the development, characterization and application of polymeric pseudophases for electrokinetic chromatography

Recent progress in the development, characterization and application of polymeric pseudophases for electrokinetic chromatography

Recent innovation in capillary electrokinetic chromatography with replaceable charged pseudostationary phases or additives

Modelling and optimization of the electrokinetic chromatographic separation of mixtures of organic anions and cations using poly(diallydimethyl- ammonium chloride) and hexanesulfonate as mixed pseudostationary phases

Contact Info

Product

Resources

About