Diffusion characteristics of fibrin films

Ho, Hsiu O.; Chen, Chau-Yang

doi:10.1016/0378-5173(93)90146-7

Cited by 11 publications

(2 citation statements)

References 9 publications

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“…Fibrin matrices can be formed under mild experimental conditions in vitro under the influence of intrinsic enzymatic processes and are hence suitable as a carrier of heat-labile drugs, proteins, and enzymes [ 15 ]. The porosity of the fibrin mesh formed in vitro is dependent on reaction conditions viz.…”

Section: Introductionmentioning

confidence: 99%

“…pH, ionic strength, and the presence of CaCl 2 suggest that the formulation parameters may be fine-tuned to control the relative availability of the entrapped pharmaceutical [ 16 , 17 ]. Additionally, these can incorporate reactive/fragile molecules such as protein and peptides without serious modifications [ 15 ]. Thus, the easy usage and handling of fibrin coupled with its biomimetic proficiencies greatly advocate its possible candidature for a promising drug delivery system [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections

et al. 2022

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Fibrin-based systems offer promises in drug and gene delivery as well as tissue engineering. We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens. In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations. In vitro efficacy of the different formulations was examined. Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound, were investigated in Swiss albino mice. While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone. Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins. Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone. The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections

et al. 2022

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Entrapment in plasma microparticles: A promising strategy for antigen delivery

2014

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We report the preparation of plasma microparticles (PMPs) from autologous blood plasma for sustained in vivo delivery of the entrapped antigens. The PMPs were prepared by high speed-stirring of calcium-enriched plasma, mixed with the antigen to be entrapped, in mineral oil. The preparation of PMPs did not necessitate addition of any external protein/enzyme nor special laboratory setup. Our results suggest that the PMPs release the entrapped invertase in a sustained manner both in vitro and in vivo, especially after crosslinking with glutaraldehyde. The preparations are reasonably stable to proteolysis and constitute strong candidates for eliciting immune response. Induction of humoral immune response by the PMP-entrapped invertase, as evident from the high antibody titers, was remarkable and comparable with that observed in animals receiving the antigen emulsified with Freund's Complete Adjuvant. Isotypic analysis of antibodies showed a Th1-biased immune response in animals administered uncrosslinked or crosslinked PMPs-entrapped invertase, especially after a booster dose. The analysis in animals of the group immunized with adjuvant-emulsified antigen suggested a combined Th1 and Th2 response. PMP-entrapment also caused high expression of surface markers (CD80 and CD86) on antigen presenting cells, as well as effector T-cells surface markers (CD4(+) and CD8(+) ) as revealed by FACS. The study suggests that PMPs offer remarkable promise as adjuvant-free and biocompatible vaccine delivery systems.

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Sustained Release of BSA from a Novel Drug Delivery Matrix – Bullfrog Skin Collagen Film

Wang

et al. 2004

Macromolecular Bioscience

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A novel drug delivery system, bullfrog skin collagen film, was employed to release bovine serum albumin (BSA). The biophysical properties of bullfrog skin collagen film used were evaluated by thermal denaturation temperature and percentage water loss. The film obtained exhibited good stability and can be taken as a good candidate for drug delivery matrix. Compared with conventional collagen from molecular weight and amino acid composition, bullfrog skin collagen showed significant differences. For safety considerations, bullfrog skin collagen has great advantages. The release kinetics of BSA from bullfrog skin collagen film was measured in vitro. The results indicated bullfrog skin collagen film could be used for sustained release of BSA. Thus, bullfrog skin collagen film can be taken as a novel drug delivery system.

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Diffusion characteristics of fibrin films

Cited by 11 publications

References 9 publications

Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections

Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections

Entrapment in plasma microparticles: A promising strategy for antigen delivery

Sustained Release of BSA from a Novel Drug Delivery Matrix – Bullfrog Skin Collagen Film

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