Diffusion Behavior of Analyte Molecules in a Nanoporous Matrix Created from Polystyrene Nanoparticles

Abstract

This study investigates the diffusion behavior of analyte molecules in a nanoporous matrix of polystyrene particles. Two approaches, steady-state current and potential step chronoamperometry were employed to calculate diffusion coefficients and compare the effect of nanoporous matrices with different diameter of pores and different method of immobilization of enzyme. Pores were created with close packing of polystyrene nanoparticles. The covalent and cross-linking immobilization were used to immobilize the enzyme. Chronoamperometric measurement was applied to detect analyte. The study found that the presence of nanoporous matrices immobilized on p-lysine affects diffusion. The potential step chronoamperometry method showed the improvement of diffusion. This study provides insight into the diffusion behavior of analyte molecules in nanoporous matrices, which can be useful in optimizing the design of amperometric biosensors.