Diffusible mating-type factors induce macrocyst development in Dictyostelium discoideum

O’Day, Danton H.; Lewis, Keith E.

doi:10.1038/254431a0

Cited by 43 publications

(26 citation statements)

References 7 publications

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“…O'Day and Lewis (1975) have previously provided evidence for at least two sexual pheromones in D. discoideum: one that directs development up to the precyst stage and another that directs the completion of development. The current data thus indicate that the former pheromone accumulates prior to the latter.…”

Section: Resultsmentioning

confidence: 98%

“…When pheromone production and response were assessed using agar plate cultures, NC4 and V12 spores from stock cultures were inoculated separately onto fresh or 24-h-old 0.1 % lactose proteose peptone (LPP) agar plates at a dilution of 2 X lo5 to 4 X lo5 spores/mL in 5.0 mL of distilled water (O'Day and Lewis 1975). The cultures were placed in the dark at 23 ?…”

Section: Assay Of Pheromone Production and Responsementioning

confidence: 99%

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Sexual pheromone accumulation and response in agar plate and liquid cultures of Dictyostelium discoideum

Morris¹,

Lewis²,

O’Day³

1982

Can. J. Microbiol.

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Sexual development in the mated cultures of Dictyostelium discoideum is initiated by one or more pheromones. In agar plate cultures of NC4, the presence of the pheromone becomes detectable by 22 h and reaches its maximal activity by 24 h. Cells of V12 from plate cultures also reach their maximal responsiveness at 24 h. In liquid culture, the NC4 pheromonal activity is detectable by 10 h and increases to a maximum at 15 h before declining so that by 30 h no activity is detectable. The V12 cells develop the competence to respond to the pheromone by 10 h and increase to a peak responsiveness by 15 h after which they lose the ability to respond. These data support earlier experiments demonstrating that sexual pheromones exist in the cellular slime moulds. The results of the liquid culture experiments in which the pheromonal induction of macrocyst formation reaches levels equivalent to 30% of mated cultures (NC4 × V12) also suggest that this pheromone bioassay system is sensitive enough to permit the isolation and characterization of the sexual pheromones of D. discoideum.

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Section: Resultsmentioning

confidence: 98%

Section: Assay Of Pheromone Production and Responsementioning

confidence: 99%

Sexual pheromone accumulation and response in agar plate and liquid cultures of Dictyostelium discoideum

Morris¹,

Lewis²,

O’Day³

1982

Can. J. Microbiol.

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“…The inhibition of macrocyst production observed in the charcoal-up treatment is consistent with the existence of a volatile activator of mating. It is possible that this volatile activator is the same substance as the volatile hormone reported previously (O'Day & Lewis, 1975;Lewis & O'Day, 1977).…”

Section: Pheromone and Inhibitor Studiesmentioning

confidence: 92%

“…Weinkauff & Filosa (1965) demonstrated that macrocyst production in D. mucoroides is dependent on some volatile factor that is not CO,. Evidence in support of a volatile hormone which can induce macrocyst formation in the V12 strain of D. discoideurn has been reported (O'Day & Lewis, 1975;Lewis & O'Day, 1977). This induction takes place in V12 even in the absence of cell contact with its mate NC4; NC4 is thought to produce this species-specific pheromone.…”

Section: Introductionmentioning

confidence: 92%

A Comparison of the Macrocyst and Fruiting Body Developmental Pathways in Dictyostelium discoideum

Bozzone

1983

Microbiology

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“…Alternatively, it develops sexually under dark and submerged conditions (Erdos et al, 1976;Nickerson et al, 1973). The mechanisms responsible for its developmental choice are currently unclear, although the involvement of several diffusible substances has been suggested (Fang et al, 1992;O'Day and Lewis, 1975;. We have been studying the molecular mechanisms of sexual cell interactions in D. discoideum.…”

mentioning

confidence: 99%

Identification and Characterization of Two Flavohemoglobin Genes in Dictyostelium discoideum.

Iijima¹,

Shimizu²,

Tanaka³

et al. 2000

Cell Struct. Funct.

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ABSTRACT. Flavohemoglobins are being identified in an expanding number of prokaryotes and unicellular eukaryotes. These molecules consist of an N-terminal hemoglobin domain and a C-terminal oxidoreductase domain, and are considered to function in storage or as sensors for O2, and in defense against oxidative stress and/ or NO. However, their physiological significance has not yet been determined. Here, we isolated and analyzed two flavohemoglobin genes of Dictyostelium discoideum, DdFHa and DdFHb, which lie close to each other in the genome. DdFHs were induced by submerged conditions, and enriched in the sexually mature cells of D. discoideum. Although they were not essential for growth or development under standard laboratory conditions, disruption of both genes caused an increase in number of large but uninuclear cells, and hypersensitivity to higher concentrations of glucose and to NO releasers. These results indicate that DdFHs are responsible for transducing NO signals to maintain normal cellular conditions against environmental stresses.

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Diffusible mating-type factors induce macrocyst development in Dictyostelium discoideum

Cited by 43 publications

References 7 publications

Sexual pheromone accumulation and response in agar plate and liquid cultures of Dictyostelium discoideum

Sexual pheromone accumulation and response in agar plate and liquid cultures of Dictyostelium discoideum

A Comparison of the Macrocyst and Fruiting Body Developmental Pathways in Dictyostelium discoideum

Identification and Characterization of Two Flavohemoglobin Genes in Dictyostelium discoideum.

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