Diffusible factors released by fibroblasts support epidermal morphogenesis and deposition of basement membrane components

Ghalbzouri, Abdoelwaheb El; Ponec, Maria

doi:10.1111/j.1067-1927.2004.012306.x

Cited by 116 publications

(89 citation statements)

References 58 publications

(123 reference statements)

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“…Keratinocytes strongly depend on secreted factors and ECM produced from fibroblasts to properly differentiate into a stratified epidermis. [46][47][48] Thus, printed dermal models were cultured for up to 7 weeks and were seeded with human dermal keratinocytes at different time points to determine the optimal seeding procedure. In the printed dermal models, ECM expression increased throughout culture time as verified with collagen type I expression.…”

Section: Discussionmentioning

confidence: 99%

Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells

et al. 2016

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“…151,154 It is clear in many instances that epithelial-mesenchymal interactions regulate basement membrane assembly by cells such as epithelium. 148,[155][156][157][158][159][160] This is particularly apparent in the skin whereby keratinocytes are not able to assemble a basement membrane structure (as determined with electron microscopy and immunofluorescent staining) unless underlying fibroblasts are present. Fibroblasts have been shown to be a producer of nidogens 148 that control interactions of laminins with collagen type IV isoforms in different basement membranes.…”

Section: Potential Role For Endothelial Cell Supporting Cells In the mentioning

confidence: 99%

Endothelial Extracellular Matrix

Davis

Senger

2005

Circulation Research

1,054

588

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Abstract-The extracellular matrix (ECM) is critical for all aspects of vascular biology. In concert with supporting cells, endothelial cells (ECs) assemble a laminin-rich basement membrane matrix that provides structural and organizational stability. During the onset of angiogenesis, this basement membrane matrix is degraded by proteinases, among which membrane-type matrix metalloproteinases (MT-MMPs) are particularly significant. As angiogenesis proceeds, ECM serves essential functions in supporting key signaling events involved in regulating EC migration, invasion, proliferation, and survival. Moreover, the provisional ECM serves as a pliable scaffold wherein mechanical guidance forces are established among distal ECs, thereby providing organizational cues in the absence of cell-cell contact. Finally, through specific integrin-dependent signal transduction pathways, ECM controls the EC cytoskeleton to orchestrate the complex process of vascular morphogenesis by which proliferating ECs organize into multicellular tubes with functional lumens. Thus, the composition of ECM and therefore the regulation of ECM degradation and remodeling serves pivotally in the control of lumen and tube formation and, finally, neovessel stability and maturation. (Circ Res. 2005;97:1093-1107.)Key Words: extracellular matrix Ⅲ endothelial cells Ⅲ angiogenesis Ⅲ vascular morphogenesis Ⅲ vessel stabilization T he extracellular matrix (ECM) provides critical support for vascular endothelium. Primarily through adhesive interactions with integrins on the endothelial cell (EC) surface, ECM provides a scaffold essential for maintaining the organization of vascular ECs into blood vessels. In addition, EC adhesion to ECM is required for EC proliferation, migration, morphogenesis, survival, and ultimately blood vessel stabilization, all of which are critical for neovascularization. The specific mechanisms through which ECM supports EC functions are complex and involve both external structural support and regulation of multiple signaling pathways within the cell, including signaling pathways that control apoptosis, proliferation, the cytoskeleton, and cell shape. Thus, through both mechanical and signaling func- tions, the ECM affects many fundamental aspects of EC biology. Moreover, the diversity of ECM components in the EC microenvironment and the diversity of mechanisms for controlling the synthesis and degradation of ECM have suggested an intricate level of complexity sufficient for ECM to exert significant and precise control over many aspects of neovascularization and blood vessel maturation. The purpose of this review is to provide an overview of the importance of ECM for the biology of vascular ECs, and it is divided into 4 parts: (1) ECM Function in Cellular Morphogenesis and Signaling; (2) ECM Function in EC Lumen Formation and the Switch to Vessel Maturation; (3) Endothelial ECM: Remodeling; and (4) Endothelial ECM Biosynthesis, Assembly, and Structural Functions: Critical Role for EC Basement Membrane Matrix in Vessel Stabilization. ...

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“…It consists of a dermal equivalent made up of fibroblasts in a collagen matrix and an epidermis that is differentiated from keratinocytes seeded on the dermal equivalent [3,4,7,15,26]. To prepare skin equivalents, dermal fibroblasts are needed not only in the proliferation and the differentiation of keratinocytes [10,35], but also in the formation of basement membrane components [2,11,14,18,20]. Basement membrane at the dermal-epidermal junction is mainly constituted with laminin, and type IV and VII collagens, adhering to basal cell via hemidesmosome composed of α 6 β 4 integrin, and to dermis via anchoring fibrils composed of type VII collagen [20].…”

mentioning

confidence: 99%

“…Basement membrane at the dermal-epidermal junction is mainly constituted with laminin, and type IV and VII collagens, adhering to basal cell via hemidesmosome composed of α 6 β 4 integrin, and to dermis via anchoring fibrils composed of type VII collagen [20]. Epithelium of skin equivalent forms a multilayered structure which is similar to that of normal skin [11,12,15,34]. In addition, skin equivalent also forms basement membrane including the expression of α 6 β 4 integrin, laminin, and type IV and VII collagens [2,4,12,20,29].…”

mentioning

confidence: 99%

Three-Dimensional Culture of Keratinocytes and the Formation of Basement Membrane for Canine Footpad Substitute

Yamazoe

Miyamoto

Hikosaka

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. A pad equivalent for a dog was prepared as a substitute for the loss of footpad. In addition to the time course of formation on epidermal morphogenesis, we investigated expressions of α 6 integrin subunit as adhesive molecule, and laminin and type IV and VII collagens as extracellular matrices of basement membrane components. Epithelium of the pad equivalent was thick enough to be easily confirmed at 5 days at the air-liquid interface, but many creases appeared on it at 7 days, and it shrank at 10 and 14 days. Keratinocytes were increased in 4 to 5 cell layers at 1 day at the air-liquid interface, differentiating into basal cell layer. Granular and corneal cell layers were confirmed until 5 days, and maintained their shape at least until 14 days. Alpha 6 integrin was expressed at almost the same fluorescent intensity as native pad tissue at 1 day at the dermal-epidermal junction. Laminin and type IV collagen were intermittently expressed at 5 and 10 days, respectively, at the dermal-epidermal junction, and at 14 days the fluorescence showed almost the same intensity as native pad tissue. The expression of type VII collagen was discontinuous at 2 days at the dermal-epidermal junction, but remained as it was at 14 days. The present findings suggested that although the formation of anchoring fibrils in basement membrane was incomplete, the pad equivalent in the dog was reconstructed similar to a native pad by epidermal morphogenesis. KEY WORDS: air-liquid interface, canine, epidermis, footpad, keratinocyte.

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Diffusible factors released by fibroblasts support epidermal morphogenesis and deposition of basement membrane components

Cited by 116 publications

References 58 publications

Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells

Standardized 3D Bioprinting of Soft Tissue Models with Human Primary Cells

Endothelial Extracellular Matrix

Three-Dimensional Culture of Keratinocytes and the Formation of Basement Membrane for Canine Footpad Substitute

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