Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi

Abstract

The aim of this study was to identify volatile and agar-diffusible antifungal metabolites produced by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from four-year-old roots of Korean ginseng with rot symptoms was identified as Bacillus velezensis based on 16S rDNA and gyrA sequences. Strain G341 inhibited mycelial growth of all phytopathogenic fungi tested. In vivo experiment results revealed that n-butanol extract of fermentation broth effectively controlled the development of rice sheath blight, tomato gray mold, tomato late blight, wheat leaf rust, barley powdery mildew, and red pepper anthracnose. Two antifungal compounds were isolated from strain G341 and identified as bacillomycin L and fengycin A by MS/MS analysis. Moreover, volatile compounds emitted from strain G341 were found to be able to inhibit mycelial growth of various phytopathogenic fungi. Based on volatile compound profiles of strain G341 obtained through headspace collection and analysis on GC-MS, dimethylsulfoxide, 1-butanol, and 3-hydroxy-2-butanone (acetoin) were identified. Taken together, these results suggest that B. valezensis G341 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.

Bacillus velezensis A2 fermentation exerts a protective effect on renal injury induced by Zearalenone in mice

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Stress-Responsive Alternative Sigma Factor SigB Plays a Positive Role in the Antifungal Proficiency of Bacillus subtilis

“…In particular, an antagonistic interaction between bacteria and fungi would be beneficial to protect plants of agronomical importance against phytopathogenic fungi that reduces the yield and quality of crops (5)(6)(7). One strategy to achieve this goal is to control soilborne plant diseases via the utilization of plant growth-promoting rhizobacteria (PGPRs), which have the ability to maintain the population of plant-pathogenic microbes below the disease threshold level in soil and plant tissues (8)(9)(10)(11)(12). A large number of biocontrol agents have been identified, but to date, Bacillus species, in particular those that belong to the B. amyloliquefaciens/B.…”

Protecting tobacco plants from O3 injury by Bacillus velezensis with production of acetoin

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