Diffuse Fluorescence Tomography

Abstract

This chapter discusses the field of diffuse fluorescence tomography in terms of fluorescence diffuse optical tomography (FDOT) and fluorescence molecular tomography (FMT). A brief overview of the forward photon propagation model is given. In addition, the techniques and challenges associated with solving the inverse problem, required for successful reconstruction, are discussed. Moreover, special attention is given to the different instrumentation used in diffuse fluorescence tomography. This includes the instrumentation associated with adequate illumination of the sample as well as efficient detection. Furthermore, the diverse applications of diffuse fluorescence tomography are explored, ranging from its use in biomarkers to preclinical applications and translational imaging. Finally, the chapter looks at the emerging technologies, which will shape the field in the near future.

Characterization of fluorescence lifetime of organic fluorophores for molecular imaging in the shortwave infrared window

“…Thanks to its high sensitivity, continuously growing library of available probes, and ability to image multiple probes simultaneously, fluorescence optical imaging techniques in the near-infrared (NIR) spectral window (700 to 1000 nm) have found numerous preclinical and clinical applications. 1 However, the resolution of these approaches is hampered by the scattering nature of tissues. As the scattering properties of tissue follow a power law in which the wavelength is the exponent, imaging further in the red has long been expected to improve imaging resolution.…”

“…Fluorescence optical imaging is an invaluable research tool that allows for non-invasive monitoring of numerous biological processes in live biological systems. Thanks to its high sensitivity, continuously growing library of available probes, and ability to image multiple probes simultaneously, fluorescence optical imaging techniques in the near-infrared (NIR) spectral window (700 to 1000 nm) have found numerous preclinical and clinical applications 1 . However, the resolution of these approaches is hampered by the scattering nature of tissues.…”

Characterization of fluorescence lifetime of organic fluorophores for molecular imaging in the SWIR window

“…Thanks to its high sensitivity, continuously growing library of available probes, and ability to image multiple probes simultaneously, fluorescence optical imaging techniques in the near-infrared spectral window (NIR) [700-1000 nm] have found numerous pre- and clinical applications. 1 However, the resolution of these approaches is hampered by the scattering nature of tissues. As the scattering properties of tissue follow a power law in which the wavelength is the exponent, imaging further in the red has long been expected to improve imaging resolution.…”