Diffraction techniques and vibrational spectroscopy opportunities to characterise bones

Bazin, Dominique; Chappard, Christine; Combes, Christèle; Carpentier, Xavier; Rouzière, Stéphan; André, G.; Matzen, Guy; Allix, Mathieu; Thiaudière, Dominique; Réguer, Solenn; Jungers, Paul; Daudon, Michel

doi:10.1007/s00198-009-0868-3

Cited by 80 publications

(55 citation statements)

References 97 publications

(94 reference statements)

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“…Raman scattering occurs when molecules within a specimen are excited by incident laser light. Vibrational motions within the molecules lead to a small fraction of the light (approximately one in 10 7 photons) losing energy and being scattered at longer wavelengths [7]. The wavelength difference between scattered and incident light corresponds to molecular vibrations and leads to bands at characteristic frequency shifts in the Raman spectrum.…”

Section: Introductionmentioning

confidence: 99%

Raman Assessment of Bone Quality

Morris

Mandair

2011

Clinical Orthopaedics &Amp; Related Research

439

477

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Background Progress in the diagnosis and prediction of fragility fractures depends on improvements to the understating of the compositional contributors of bone quality to mechanical competence. Raman spectroscopy has been used to evaluate alterations to bone composition associated with aging, disease, or injury. Questions/purposes In this survey we will (1) review the use of Raman-based compositional measures of bone quality, including mineral-to-matrix ratio, carbonateto-phosphate ratio, collagen quality, and crystallinity; (2) review literature correlating Raman spectra with biomechanical and other physiochemical measurements and with bone health; and (3) discuss prospects for ex vivo and in vivo human subject measurements. Methods ISI Web of Science was searched for references to bone Raman spectroscopy in peer-reviewed journals. Papers from other topics have been excluded from this review, including those on pharmaceutical topics, dental tissue, tissue engineering, stem cells, and implant integration. Results Raman spectra have been reported for human and animal bone as a function of age, biomechanical status, pathology, and other quality parameters. Current literature supports the use of mineral-to-matrix ratio, carbonate-tophosphate ratio, and mineral crystallinity as measures of bone quality. Discrepancies between reports arise from the use of band intensity ratios rather than true composition ratios, primarily as a result of differing collagen band selections. Conclusions Raman spectroscopy shows promise for evaluating the compositional contributors of bone quality in ex vivo specimens, although further validation is still needed. Methodology for noninvasive in vivo assessments is still under development.

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Section: Introductionmentioning

confidence: 99%

Raman Assessment of Bone Quality

Morris

Mandair

2011

Clinical Orthopaedics &Amp; Related Research

439

477

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“…In the past decades, RS has been successfully applied to characterize alterations in bone composition caused by aging and disease. [10][11][12][13] McCreadie et al studied women proximal femurs and suggested bone compositions as risk factors for osteoporotic fracture. 10 In another study, RS-derived bone material properties have exhibited significant correlation with the tissue-level mechanical function of bone, demonstrating promising potential in fracture risk prediction.…”

Section: Introductionmentioning

confidence: 99%

Development of Raman spectral markers to assess metastatic bone in breast cancer

et al. 2014

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Abstract. Bone is the most common site for breast cancer metastases. One of the major complications of bone metastasis is pathological bone fracture caused by chronic bone loss and degeneration. Current guidelines for the prediction of pathological fracture mainly rely on radiographs or computed tomography, which are limited in their ability to predict fracture risk. The present study explored the feasibility of using Raman spectroscopy to estimate pathological fracture risk by characterizing the alterations in the compositional properties of metastatic bones. Tibiae with evident bone destruction were investigated using Raman spectroscopy. The carbonation level calculated by the ratio of carbonate/phosphate ν1 significantly increased in the tumor-bearing bone at all the sampling regions at the proximal metaphysis and diaphysis, while tumor-induced elevation in mineralization and crystallinity was more pronounced in the metaphysis. Furthermore, the increased carbonation level is positively correlated to bone lesion size, indicating that this parameter could serve as a unique spectral marker for tumor progression and bone loss. With the promising advances in the development of spatially offset Raman spectroscopy for deep tissue measurement, this spectral marker can potentially be used for future noninvasive evaluation of metastatic bone and prediction of pathological fracture risk.

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“…Among other techniques, diffraction measurements with X-rays, synchrotron, or neutron radiation (25)(26)(27)(28)(29) can be used to quantify the mineral properties in bone or fracture callus. Wen and colleagues (30) combined scanning electron microscopy (SEM), X-ray diffraction (XRD), and transmission electron microscopy (TEM) and proposed an evolutionary model for human callus formation that contains five representative stages of collagenmineral organization, where fusion of mineralized fibrils is followed by the absorption of disordered and the deposition of ordered collagen matrix.…”

mentioning

confidence: 99%

Size and habit of mineral particles in bone and mineralized callus during bone healing in sheep

Liu

Manjubala

Schell

et al. 2010

Journal of Bone and Mineral Research

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Bone healing is known to occur through the successive formation and resorption of various tissues with different structural and mechanical properties. To get a better insight into this sequence of events, we used environmental scanning electron microscopy (ESEM) together with scanning small-angle X-ray scattering (sSAXS) to reveal the size and orientation of bone mineral particles within the regenerating callus tissues at different healing stages (2, 3, 6, and 9 weeks). Sections of 200 mm were cut from embedded blocks of midshaft tibial samples in a sheep osteotomy model with an external fixator. Regions of interest on the medial side of the proximal fragment were chosen to be the periosteal callus, middle callus, intercortical callus, and cortex. Mean thickness (T parameter), degree of alignment (r parameter), and predominant orientation (c parameter) of mineral particles were deduced from resulting sSAXS patterns with a spatial resolution of 200 mm. 2D maps of T and r overlapping with ESEM images revealed that the callus formation occurred in two waves of bone formation, whereby a highly disordered mineralized tissue was deposited first, followed by a bony tissue with more lamellar appearance in the ESEM and where the mineral particles were more aligned, as revealed by sSAXS. As a consequence, degree of alignment and mineral particle size within the callus increased with healing time, whereas at any given moment there were structural gradients, for example, from periosteal toward the middle callus. ß

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Diffraction techniques and vibrational spectroscopy opportunities to characterise bones

Cited by 80 publications

References 97 publications

Raman Assessment of Bone Quality

Raman Assessment of Bone Quality

Development of Raman spectral markers to assess metastatic bone in breast cancer

Size and habit of mineral particles in bone and mineralized callus during bone healing in sheep

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