Diffraction phase microscopy: principles and applications in materials and life sciences

Bhaduri, Basanta; Edwards, Chris; Pham, Hoa V.; Zhou, Renjie; Nguyen, Tuan Hung; Goddard, Lynford L.; Popescu, Gabriel

doi:10.1364/aop.6.000057

Cited by 335 publications

(231 citation statements)

References 114 publications

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“…The reference and sample beams are collected by the second lens (L2) such that they interfere at the EMCCD (Andor, iXon3). The obtained interference pattern was processed as described in [13]. The phase temporal and spatial stability of the system were measured to be 50 mrad and 40 mrad, respectively.…”

Section: Imagingmentioning

confidence: 99%

“…While a number of QPI approaches for visualizing neurons and brain tissues have been introduced, e.g., digital holographic microscopy [10,11], optical coherence phase microscopy [12], diffraction phase microscopy (DPM) [13,14], spatial light interference microscopy [15,16], they all utilize interferometry as the underlying operating principle. From the interferogram, the alteration in optical pathlength due to tissue is obtained with nanoscale sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…This level of sensitivity is difficult to achieve with any other method. However, QPI has been limited to visualizing cultured neurons and very thin histological section (4-5 microns) [10][11][12][13][14][15][16]. The reason is that the light scattering prevents imaging thick tissues.…”

Section: Introductionmentioning

confidence: 99%

“…DPM [13,17] is a particular type of QPI method, of very high stability, granted by the common-path interferometric geometry. Due to its intrinsic temporal sensitivity to phase changes, DPM is used in biomedical applications for measuring cell dynamics [18] and brain imaging [14].…”

Section: Introductionmentioning

confidence: 99%

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Measurement of multispectral scattering properties in mouse brain tissue

Min

Ban

Wang

et al. 2017

Biomed. Opt. Express

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Abstract:We present the scattering properties of mouse brain using multispectral diffraction phase microscopy. Typical diffraction phase microscopy was incorporated with the broadband light source which offers the measurement of the scattering coefficient and anisotropy in the spectral range of 550-900 nm. The regional analysis was performed for both the myeloarchitecture and cytoarchitecture of the brain tissue. Our results clearly evaluate the multispectral scattering properties in the olfactory bulb and corpus callosum. The scattering coefficient measured in the corpus callosum is about four times higher than in the olfactory bulb. It also indicates that it is feasible to realize the quantitative phase microscope in near infrared region for thick brain tissue imaging.

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Section: Imagingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Measurement of multispectral scattering properties in mouse brain tissue

Min

Ban

Wang

et al. 2017

Biomed. Opt. Express

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“…Here we fabricated nanoscale Pd-coated cantilevers and characterized the devices using diffraction phase microscopy (DPM) [37][38][39][40][41][42][43] . The DPM technique achieves real-time single-shot non-destructive quantitative phase imaging in situ with nanometer-level sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Realization of palladium-based optomechanical cantilever hydrogen sensor

McKeown

Wang

et al. 2017

Microsyst Nanoeng

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Hydrogen has attracted attention as an alternative fuel source and as an energy storage medium. However, the flammability of hydrogen at low concentrations makes it a safety concern. Thus, gas concentration measurements are a vital safety issue. Here we present the experimental realization of a palladium thin film cantilever optomechanical hydrogen gas sensor. We measured the instantaneous shape of the cantilever to nanometer-level accuracy using diffraction phase microscopy. Thus, we were able to quantify changes in the curvature of the cantilever as a function of hydrogen concentration and observed that the sensor's minimum detection limit was well below the 250 p.p.m. limit of our test equipment. Using the change in curvature versus the hydrogen curve for calibration, we accurately determined the hydrogen concentrations for a random sequence of exposures. In addition, we calculated the change in film stress as a function of hydrogen concentration and observed a greater sensitivity at lower concentrations.Keywords: hydrogen detection; imaging and sensing; interference microscopy; optomechanics; optical sensors; surface dynamics INTRODUCTIONHydrogen has always been viewed as a promising alternative to fossil fuels, and it can also function as an effective energy storage medium for intermittent energy sources. In addition, hydrogen is used in a range of other industries, including chemical production, metal refining, and food processing. A major safety concern with hydrogen is combustibility. Therefore, early leak detection and concentration determination of hydrogen have been areas of intense research 1-3 . There are various types of hydrogen sensors that use a wide range of detection mechanisms. Lundström et al. 4 proposed metal oxide semiconductor (MOS)-type hydrogen sensors 5 . However, MOS sensors suffer from drawbacks such as premature saturation of detectable hydrogen concentrations and low sensitivity. Other MOS-based devices have been used as hydrogen sensors, such as MOS field-effect transistors (FETs) 6,7 , high electron mobility transistors [8][9][10] , and Schottky diode-type FETs 11,12 . However, these devices require complicated fabrication processes and have high production costs. Optical gas sensors 13-21 not only overcome these disadvantages but also have other unique advantages, such as negligible electrical interference, no risk of ignition from an electrical spark, and the ability to work at high temperatures or in harsh environments.Palladium (Pd) can absorb up to 900 times its own weight in hydrogen gas at room temperature 22 . Compared with platinum 23 , Pd film is more popular because of its lower cost. Pd alloys with Ag, Au, Ni, and WO 3 have also been studied 24-29 because of their improved response time 24,25,27 , sensitivity 28,29 , and durability 26 as a sensing material. The alloys can avoid blistering effects 26 and the α to β phase transition at higher hydrogen concentrations 25,27 .

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