Diffractaic acid, a novel proapoptotic agent, induces with olive oil both apoptosis and antioxidative systems in Ti-implanted rabbits

Odabaşoğlu, Fehmi; Yıldırım, Ömer Selim; Aygün, Hayati; Halıcı, Zekai; Halici, Mesut; Erdoğan, Fazlı; Çadırcı, Elif; Çakır, Ahmet; Okumuş, Zafer; Aksakal, Bünyamin; Aslan, Ali; Ünal, Deniz; Bayır, Yasin

doi:10.1016/j.ejphar.2011.11.019

Cited by 23 publications

(28 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, a study by Odabasoglu et al . demonstrated the pro‐apoptotic role of olive oil by activation of caspase‐2, −3, −8 and − 9 in Ti‐implanted rabbits . Moreover, they provided an evidence, which showed that besides caspase‐dependent mechanism, olive oil‐induced apoptosis was also caused by its antioxidant property.…”

Section: Discussionmentioning

confidence: 89%

“…However, a study by Odabasoglu et al demonstrated the pro-apoptotic role of olive oil by activation of caspase-2, −3, −8 and − 9 in Ti-implanted rabbits. 52 Moreover, they provided an evidence, which showed that besides caspase-dependent mechanism, olive oil-induced apoptosis was also caused by its antioxidant property. Recently, Leong et al revealed that a natural product (Aglaia exima) triggered apoptosis by activation of caspase cascade and inhibiting NF-kB translocation in colon cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Chemopreventive role of olive oil in colon carcinogenesis by targeting noncoding RNAs and methylation machinery

Nanda

Mahmood

Bhatia

et al. 2018

Intl Journal of Cancer

View full text Add to dashboard Cite

Epigenetic therapy induced by dietary components has become a strong interest in the field of cancer prevention. Olive oil, a potent dietary chemopreventive agent, control colon cancer, however, its role in epigenetic therapy remains unclear. Thus, we aimed to investigate the effect of olive oil in a preclinical model of colon cancer by targeting genetic and epigenetic mechanisms. DMH was used to induce colon cancer in rats; while olive oil was given to separate group of rats along with DMH treatment. Tumor burden and incidence in DMH and DMH + olive oil-treated rats was observed by macroscopic examination and histoarchitectural studies. Potent anti-inflammatory, anti-angiogenic and pro-apoptotic activity of olive oil was explored by gene expression and immunohistochemical studies. The effect of olive oil on epigenetic alterations was examined by detecting promoter methylation with MS-HRM and dysregulation of miRNA by TaqMan MicroRNA Assay. We observed that olive oil administration lowered tumor incidence and inhibited the development of tumors in DMH-treated rats. Olive oil markedly decreased the expression of inflammatory and angiogenic markers and restored the expression of pro-apoptotic markers in DMH-treated rats. Furthermore, the inverse relationship between gene expression and DNA methylation, deviant miRNA pattern and miRNA silencing mediated by aberrant DNA methylation was also seen in DMH-treated rats, which was potentially reversible upon olive oil treatment. Our study concludes that olive oil may play a role in the epigenetic therapy by altering NF-κB and apoptotic pathways via targeting noncoding RNAs and methylation machinery that affecting epigenome to prevent colon carcinogenesis.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Chemopreventive role of olive oil in colon carcinogenesis by targeting noncoding RNAs and methylation machinery

Nanda

Mahmood

Bhatia

et al. 2018

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…Aerobic organisms contributes in enzymatic and non‐enzymatic antioxidant defence systems against reactive oxygen species. These antioxidants can be listed as; catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPO), reduced glutathione (GSH), glutathione reductase (GR), glutathione S ‐transferase (GST), vitamins and flavonoids [Odabasoglu et al, , ]. Furthermore, antioxidative properties of the various anti‐secretory drugs such as histamine H 2 receptor antagonists and proton pump inhibitors are also shown [Farzin and Attarzadeh, ].…”

mentioning

confidence: 99%

“…Two cycloocxygenase enxymnes, Cox-1 and COX-2 converst arachidonic acid into prostanoids in definite ratios. Nonsteroidal anti-inflammatory drugs (NSAIDs) which used to reduced fever, pain and inflammation inhibits COX enzymes [FitzGerald and Patrono, 2001;Odabasoglu et al, 2007Odabasoglu et al, , 2008Odabasoglu et al, , 2012 resulting in inhibited prostaglandin synthesis. Therefore gastric lesions revealed as an important adverse effect by inhibiting prostaglandin biosynthesis [Koc et al, 2008;Karakus et al, 2009].…”

mentioning

confidence: 99%

“…Hydroxyl radicals (OH • ) or superoxide anions (O 2 À•) and hydrogen peroxide (H 2 O 2 ), which are reactive oxygen species, cause lipid peroxidation in tissues [Elliot and Wallace, 1998;Karaca et al, 2009;Odabasoglu et al, 2012]. Oxidative stress also contributes in the development of many different pathologies such as local or systemic inflammatory disorders, respiratory disorders, rheumatoid arthritis, atherosclerosis, and cancer [Kumtepe et al, 2010;Albayrak et al, 2010Albayrak et al, , 2011Odabasoglu et al, 2012;Cimini et al, 2015]. Aerobic organisms contributes in enzymatic and non-enzymatic antioxidant defence systems against reactive oxygen species.…”

mentioning

confidence: 99%

See 1 more Smart Citation

N-Acetyl Cysteine Has Both Gastro-Protective and Anti-Inflammatory Effects in Experimental Rat Models: Its Gastro-Protective Effect Is Related to Its In Vivo and In Vitro Antioxidant Properties

et al. 2015

Self Cite

View full text Add to dashboard Cite

N-acetyl cysteine (NAC), a metabolite of sulphur-containing amino acid cysteine, is used as an antioxidant and a mucolytic agent. Therefore, we aimed to investigate anti-inflammatory and anti-ulcerative effects of NAC. We also intended to determine the relation between antiulcer effect of NAC and its antioxidant properties by biochemical evaluation. In this study a total of 15 rat groups (n = 6 per group) were used for inflammation and ulcer experiments. Anti-inflammatory effects of NAC have been investigated on six rat groups with carrageenan (CAR)-induced paw oedema model. Antiulcer effects of NAC have been investigated on 24 h fasted nine rat groups with IND-induced ulcer model in the presence of positive (LAN, RAN, FAM, and OMEP), negative (untreated IND group) and intact control groups. In biochemical analyses of stomach tissues; glutathione S-transferase (GST), catalase (CAT), myeloperoxidase (MPO), and superoxide dismutase (SOD) enzyme activities and lipid peroxidation (LPO) and the glutathione (GSH) levels were determined. All doses of NAC exerted significant anti-inflammatory effect; even the effect of 900 mg/kg NAC was similar with that of DIC and IND. In gastric tissues NAC administration decreased the level of LPO and activity of CAT, which were increased by IND. Furthermore, NAC increased the GSH level and SOD and GST activities, which decreased in ulcerous stomach tissues. Only MPO activity increased in both IND and NAC groups when compared to healthy rat group. We determined that NAC has both anti-inflammatory and anti-ulcerative effects.

show abstract

Diffractaic acid exhibits thioredoxin reductase 1 inhibition in lung cancer A549 cells

Günaydın

Sulukoğlu

Kalın

et al. 2023

J of Applied Toxicology

View full text Add to dashboard Cite

Lung cancer is the leading cause of cancer‐related deaths all over the world. Therefore, it has gained importance in the development of new chemotherapeutic strategies to identify anticancer agents with low side effects, reliable, high anticancer potential, and specific to lung cancer cells. Thioredoxin reductase 1 (TrxR1) is an important therapeutic target for lung cancer treatment because of its overexpression in tumor cells. Here, we aimed to examine the anticancer effect of diffractaic acid, a lichen secondary metabolite, in A549 cells by comparing it with the commercial chemotherapeutic drug carboplatin and also to investigate whether the anticancer effect of diffractaic acid occurs via TrxR1‐targeting. The IC50 value of diffractaic acid on A549 cells was determined as 46.37 μg/mL at 48 h, and diffractaic acid had stronger cytotoxicity than carboplatin in A549 cells. qPCR results revealed that diffractaic acid promoted the intrinsic apoptotic pathway through the upregulation of the BAX/BCL2 ratio and P53 gene in A549 cells, which is consistent with the flow cytometry results. Furthermore, migration analysis results indicated that diffractaic acid impressively suppressed the migration of A549 cells. While the enzymatic activity of TrxR1 was inhibited by diffractaic acid in A549 cells, no changes were seen in the quantitative expression levels of gene and protein. These findings provide fundamental data on the anticancer effect of diffractaic acid on A549 cells targeting TrxR1 activity, suggesting that it could be considered a chemotherapeutic agent for lung cancer therapy.

show abstract

Diffractaic acid, a novel proapoptotic agent, induces with olive oil both apoptosis and antioxidative systems in Ti-implanted rabbits

Cited by 23 publications

References 51 publications

Chemopreventive role of olive oil in colon carcinogenesis by targeting noncoding RNAs and methylation machinery

Chemopreventive role of olive oil in colon carcinogenesis by targeting noncoding RNAs and methylation machinery

N-Acetyl Cysteine Has Both Gastro-Protective and Anti-Inflammatory Effects in Experimental Rat Models: Its Gastro-Protective Effect Is Related to Its In Vivo and In Vitro Antioxidant Properties

Diffractaic acid exhibits thioredoxin reductase 1 inhibition in lung cancer A549 cells

Contact Info

Product

Resources

About