Difficulty in removing biofilm from dry surfaces

Parvin, Farhana; Hu, Honghua; Whiteley, Greg S.; Glasbey, Trevor; Vickery, Karen

doi:10.1016/j.jhin.2019.07.005

Cited by 21 publications

(19 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The spread of bacteria from one surface to another by microfibre cloth has also been described previously and questions the use of this material for environmental cleaning (Bergen et al, 2009). Parvin et al (2019) investigated the ability of cloth moistened with water to remove S. aureus DSB and observed a 1.48 log 10 reduction, even with wiping the DSB surface up to 50 times. This reduction is much lower than the results presented here (≤3 log 10 reduction with water control), which may be explained with differences in methodology and wipe materials.…”

Section: Discussionmentioning

confidence: 99%

“…This reduction is much lower than the results presented here (≤3 log 10 reduction with water control), which may be explained with differences in methodology and wipe materials. In our study, DSB are formed by sedimentation in well plates, whilst Parvin et al (2019) used the CDC Biofilm Reactor. In their study a viscose/ polyester blend material was also used to wipe surfaces.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dual species dry surface biofilms; Bacillus species impact on Staphylococcus aureus survival and surface disinfection

Centeleghe

Norville²,

Hughes

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: Dry surface biofilms (DSB) survive on environmental surfaces throughout hospitals, able to resist cleaning and disinfection interventions. This study aimed to produce a dual species DSB and explore the ability of commercially available wipe products to eliminate pathogens within a dual species DSB and prevent their transfer. Methods and Results:Staphylococcus aureus was grown with two different species of Bacillus on stainless steel discs, over 12 days using sequential hydration and dehydration phases. A modified version of ASTM 2967-15 was used to test six wipe products including one water control with the Fitaflex Wiperator. Staphylococcus aureus growth was inhibited when combined with Bacillus subtilis. Recovery of S. aureus on agar from a dual DSB was not always consistent. Our results did not provide evidence that Bacillus licheniformis protected S. aureus from wipe action. There was no significant difference of S. aureus elimination by antimicrobial wipes between single and dual species DSB. B. licheniformis was easily transferred by the wipe itself and to new surfaces both in a single and dual species DSB, whilst several wipe products inhibited the transfer of S. aureus from wipe. However, S. aureus direct transfer to new surfaces was not inhibited post-wiping. Conclusions:Although we observed that the dual DSB did not confer protection of S. aureus, we demonstrated that environmental species can persist on surfaces after disinfection treatment. Industries should test DSB against future products and hospitals should consider carefully the products they choose. Significance and Impact of the Study:To our knowledge, this is the first study reporting on the production of a dual species DSB. Multispecies DSB have been identified throughout the world on hospital surfaces, but many studies focus on single species biofilms. This study has shown that DSB behave differently to hydrated biofilms.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dual species dry surface biofilms; Bacillus species impact on Staphylococcus aureus survival and surface disinfection

Centeleghe

Norville²,

Hughes

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…Interpretation of inconsistently high results is problematic and confounding to statistical analysis, particularly where microbial estimation is used in tandem with ATP testing [14,15]. Work has shown that whereas simple dried colonies of bacterial contaminants may be easily wiped away, dry surface biofilms are very difficult to remove through a standard wiping technique [16].…”

Section: Introductionmentioning

confidence: 99%

Using a simplified ATP algorithm to improve data reliability and improve cleanliness standards for surface and medical device hygiene

Whiteley

Glasbey

Fahey

2022

Infection, Disease & Health

View full text Add to dashboard Cite

Background: An algorithm has been improved to mitigate variability in cleanliness measurements of various surfaces using rapid Adenosine Triphosphate (ATP) testing. A cleaning intervention step (CIS) verifies the cleanability of those surfaces. Methods: ATP testing was performed on surfaces which were pre-approved as "clean" and ready for re-use. Adjacent (duplicate) ATP sampling was undertaken on 421 environmental surfaces, medical devices and other implements. The CIS was conducted on 270 surfaces using an aseptic technique and disposable cleaning wipes. Results: The two initial ATP results were plotted against each other with a 100 RLU threshold grading the results as clean (2x < 100RLU), dirty (2x > 100RLU) or equivocal (1x < 100RLU and 1x > 100RLU). Of the surfaces sampled, 68.5 % were clean (288/421), 13.5 % were dirty (57/ 421) and 18 % were equivocal (76/421). The duplicate testing demonstrated a false negative rate of 10 % (44/421) where the first swab was <100 RLU and the second swab >100 RLU. For the equivocal group, the gap between the two swabs was >100 RLU for 7.5 % of surfaces (33/421). The CIS was conducted on 270 of the surfaces tested and showed that cleaning could be improved (PZ<0.001) on 88.5 % of surfaces (239/270). Conclusion:The simplified ATP testing algorithm provides real-time discrimination between surface cleanliness levels and improved certainty over surface hygiene. The duplicate swab sampling approach mitigates uncontrolled variability in the results and the CIS provides a nuanced understanding of the measurable cleanliness of any surface.

show abstract

“…Biofilms are responsible for up to 80% of all causes of chronic and recurrent bacterial infections in humans [ 1 ]. The presence of biofilms has been confirmed on more than 90% of dry healthcare surfaces in intensive care units [ 2 ]. Currently, the presence of antibiotic-resistant bacteria and the infections caused by them are a huge threat to public health.…”

Section: Introductionmentioning

confidence: 99%

The Enhancement of Antimicrobial Photodynamic Therapy of Escherichia Coli by a Functionalized Combination of Photosensitizers: In Vitro Examination of Single Cells by Quantitative Phase Imaging

Pietrowska

Hołowacz

Ulatowska-Jarża

et al. 2022

IJMS

View full text Add to dashboard Cite

The prevention of biofilm formation is crucial for the limitation of bacterial infections typically associated with postoperative infections, complications in bedridden patients, and a short-term prognosis in affected cancer patients or mechanically ventilated patients. Antimicrobial photodynamic therapy (aPDT) emerges as a promising alternative for the prevention of infections due to the inability of bacteria to become resistant to aPDT inactivation processes. The aim of this study was to demonstrate the use of a functionalized combination of Chlorin e6 and Pheophorbide as a new approach to more effective aPDT by increasing the accumulation of photosensitizers (PSs) within Escherichia coli cells. The accumulation of PSs and changes in the dry mass density of single-cell bacteria before and after aPDT treatment were investigated by digital holotomography (DHT) using the refractive index as an imaging contrast for 3D label-free live bacteria cell imaging. The results confirmed that DHT can be used in complex examination of the cell–photosensitizer interaction and characterization of the efficiency of aPDT. Furthermore, the use of Pheophorbide a as an efflux pomp inhibitor in combination with Chlorin e6 increases photosensitizers accumulation within E. coli and overcomes the limited penetration of Gram-negative cells by anionic and neutral photosensitizers.

show abstract

Difficulty in removing biofilm from dry surfaces

Cited by 21 publications

References 13 publications

Dual species dry surface biofilms; Bacillus species impact on Staphylococcus aureus survival and surface disinfection

Dual species dry surface biofilms; Bacillus species impact on Staphylococcus aureus survival and surface disinfection

Using a simplified ATP algorithm to improve data reliability and improve cleanliness standards for surface and medical device hygiene

The Enhancement of Antimicrobial Photodynamic Therapy of Escherichia Coli by a Functionalized Combination of Photosensitizers: In Vitro Examination of Single Cells by Quantitative Phase Imaging

Contact Info

Product

Resources

About