Difficidin class of polyketide antibiotics from marine macroalga-associated Bacillus as promising antibacterial agents

Chakraborty, Kajal; Kizhakkekalam, Vinaya Kizhakkepatt; Joy, Minju; Dhara, Shubhajit

doi:10.1007/s00253-021-11390-z

Cited by 28 publications

(24 citation statements)

References 33 publications

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“…For instance, a study reported Bacillus amyloliquefaciens that was isolated from the seaweed Hypnea valentiae and this bacterial isolate synthesized novel amylomacins antibiotics against S. aureus [15]. Moreover, another study highlighted the polyketide difficidin synthesized by B. amyloliquefaciens that was isolated from Kappaphycus alverezii and the compound was active against multidrug resistant S. aureus, K. pneumoniae, Enterococcus faecalis and pseudomonas aeruginosa [16].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial, antifungal and antioxidant activities of Bacillus cereus SMPRL-2 isolated from seaweeds Eucheuma cottonii

Indraningrat,

Purnami,

Aryastuti

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Seaweeds have a strong relationship with prokaryotic community especially bacteria. Bacteria-associated with seaweeds generally produce active compounds which could be potential for pharmaceutical purposes. This research was focused to study antibacterial, antifungal and antioxidant activities from a bacteria encoded as SMPRL-2, isolated from Eucheuma cottonii. Molecular identification of the isolate was performed based on 16S rRNA sequencing and its morphology was assessed using scanning electronic microscope (SEM). The isolate SMPRL-2 was fermented in 100 mL ISP-2 liquid media for 14 days and extracted using ethyl acetate (1:1 ratio, v/v). The obtained crude extract was screened against bacterial and fungal isolates based on Kirby-Bauer method. The extract was screened for its antioxidant activities using DPPH method. The isolate was identified as Bacillus cereus, with short rod morphology under SEM observation. Zone of inhibition (ZOI) of the extract was 14.7±0.8 mm and 9.1±0.8 mm against Streptococcus mutans FNCC 0405 and Escherichia coli ATCC 25922, respectively. Furthermore, ZOI of the extract against Candida albicans and Aspergillus flavus were 9.6±0.9 mm and 8.8±2.3 mm respectively. A very weak antioxidant activity with IC50 value of 33000 ppm was observed. Overall, this result provides a preliminary finding of marine bacteria as naturally active compounds producer.

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Section: Discussionmentioning

confidence: 99%

Antibacterial, antifungal and antioxidant activities of Bacillus cereus SMPRL-2 isolated from seaweeds Eucheuma cottonii

Indraningrat,

Purnami,

Aryastuti

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…AntiSMASH v. 6.1.1 was used to identify secondary metabolites biosynthesis gene clusters, setting the detection strictness as relaxed ( 8 ). Interestingly, we predicted genes encoding secondary metabolites with known antibiotic activity, such as bacilysin ( 9 ), difficidin ( 10 ), bacillaene ( 11 ), macrolactin H ( 12 ), surfactin ( 13 ), and fengycin ( 14 ) ( Table 1 ). This indicates that BIB0110 harbors important genes to promote the biocontrol of pathogens with more sustainable approaches.…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequence of the Plant Growth Promoter and Biocontrol Agent Bacillus velezensis Strain BIB0110

Carvalho

Apaza-Castillo

Paz

et al. 2023

Microbiol Resour Announc

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“…Among these polyketide-spanned macrolides, compound 13 had the lowest MIC value (1.56 μg/mL), and its in silico docking study with SaPDF demonstrated a binding energy of 12.61 kcal/mol as well as an inhibition constant (K i ) of 573.34 pM, implying that it is a promising antibiotic lead compound. Twenty-one membered macrocyclic lactones difficidin analogues 19 ~ 22 , also isolated from the marine bacterium B. amyloliquefaciens MTCC12713, disclosed their significant bactericidal activities with clearance zone of 17 mm, 26 mm, 23 mm and 25 mm, respectively [ 39 ]. Among them, compound 20 bearing 9-methyl-19-propyl dicarboxylate demonstrated MIC value of 4 nM against P. aeruginosa and drug-likeness score of 0.35.…”

Section: Emerging Mnps As Promising Antibiotics For Inhibiting ...mentioning

confidence: 99%

Discovery of Marine Natural Products as Promising Antibiotics against Pseudomonas aeruginosa

Maimaitiming

Zhou

et al. 2022

Marine Drugs

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Pseudomonas aeruginosa, one of the most intractable Gram-negative bacteria, has become a public health threat due to its outer polysaccharide layer, efflux transporter system, and high level of biofilm formation, all of which contribute to multi-drug resistance. Even though it is a pathogen of the highest concern, the status of the antibiotic development pipeline is unsatisfactory. In this review, we summarize marine natural products (MNPs) isolated from marine plants, animals, and microorganisms which possess unique structures and promising antibiotic activities against P. aeruginosa. In the last decade, nearly 80 such MNPs, ranging from polyketides to alkaloids, peptides, and terpenoids, have been discovered. Representative compounds exhibited impressive in vitro anti-P. aeruginosa activities with MIC values in the single-digit nanomolar range and in vivo efficacy in infectious mouse models. For some of the compounds, the preliminary structure-activity-relationship (SAR) and anti-bacterial mechanisms of selected compounds were introduced. Compounds that can disrupt biofilm formation or membrane integrity displayed potent inhibition of multi-resistant clinical P. aeruginosa isolates and could be considered as lead compounds for future development. Challenges on how to translate hits into useful candidates for clinical development are also proposed and discussed.

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Difficidin class of polyketide antibiotics from marine macroalga-associated Bacillus as promising antibacterial agents

Cited by 28 publications

References 33 publications

Antibacterial, antifungal and antioxidant activities of Bacillus cereus SMPRL-2 isolated from seaweeds Eucheuma cottonii

Antibacterial, antifungal and antioxidant activities of Bacillus cereus SMPRL-2 isolated from seaweeds Eucheuma cottonii

Draft Genome Sequence of the Plant Growth Promoter and Biocontrol Agent Bacillus velezensis Strain BIB0110

Discovery of Marine Natural Products as Promising Antibiotics against Pseudomonas aeruginosa

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