Differntial effect of cycloheximide on the short term gibberellin and auxin growth kinetics of gamma-coleoptiles

Rose, R. J.

doi:10.1016/0304-4211(74)90121-7

Cited by 12 publications

(13 citation statements)

References 18 publications

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“…This finding agrees well with the resuhs of Rose (1974), who conducted short-term experiments with excised coleoptiles from gamma-piantlets. He analyzed the influence of cycloheximide on GA induced elongation by use of a transducer and determined a lag period of about 50 min.…”

Section: Parametersupporting

confidence: 90%

“…It is well known that elongation in coleoptiles from gannma-plantlets is dependent on protein synthesis (Kaufman et al 1968, Rose 1974. From experiments vrith inhibitors of RNA synthesis we concluded that also transcriptions are necessary for GA stimulated elongation (unpublished).…”

Section: Resultsmentioning

confidence: 88%

“…It is well known that irradiation suppresses cell divisions and damages the apparatus for DNA synthesis. Since Haber and Luippold (1960) had shown that growth of these plantlets is performed exclusively by cell elongation, many publications appeared which in general confirm that gaouna-plantlets behave remarkably similarly to normal plants (literature, see Haber et al 1961,1969, Schwarz and Haber 1973, Rose and Adamson 1969, Rose 1974). Among the properties concerned the response of gamma-plantlets or their excised coleoptiles to phytohormones has been investigated in different aspects.…”

Section: Introductionmentioning

confidence: 95%

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Action of Cordycepin on Gibberellic Acid Stimulated Cell Elongation in Coleoptiles from Gamma‐Plantlets of Wheat

Kern

1975

Physiologia Plantarum

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Section: Parametersupporting

confidence: 90%

Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 95%

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Action of Cordycepin on Gibberellic Acid Stimulated Cell Elongation in Coleoptiles from Gamma‐Plantlets of Wheat

Kern

1975

Physiologia Plantarum

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“…Induction times of growth by auxin have been reported at about 10 min in several growth systems (7). Far fewer studies have been made of GA3-induced growth, but average induction times have been reported for decapitated pea seedlings at 24 min (21) and 17 min (6), for morning glory seedlings at 14 min (2), for wheat coleoptile segments at 50 min (18), and for lettuce hypocotyl sections at 10 to 15 min (15 A possible explanation for the reduction in response time to GA3 in oat stem segments is that a factor, required for GA3-induced growth, is produced in the tissue more rapidly in the presence of exogenous IAA. It is worth noting that in a study of the pH of the solution surrounding oat stem segments, Hebard et al (8) found that after 10 min of GA3 treatment, the rate of Plant Physiol.…”

Section: Discussionmentioning

confidence: 99%

Interaction of Indoleacetic Acid and Gibberellic Acid in the Short-Term Growth Kinetics of Oat Stem Segments

Adams¹,

Ross²

1983

Plant Physiol.

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Gibberellins and auxins are the only hormones known that promote growth in oat (Arena sativa) stem segments, but when applied together, indoleacetic acid inhibits gibberellic acid-induced growth appreciably. This study shows that in addition to this inhibitory role, indoleacetic acid shortens the response time of the tissue to gibberellic acid.Stem segments taken from the next-to-last internode of oats (Avena) are particularly responsive to gibberellins. Under optimal conditions, 1-cm segments treated with GA3 grow 7.5 cm in 3 d, a value 21 times that of control growth (1). This response may be due exclusively to GA3 since IAA (1 1, 14, 16), kinetin (9), ABA (13), and ethylene (Adams, unpublished data) partially inhibit the GA3-promoted growth. IAA, however, is distinctive among these GA3 antagonists in that it alone promotes growth in the absence of GA3. Growth promotion by IAA is reported to be from 40% to 67% (11, 14, 16).Because of this special role of IAA and GA3 in oat stem segments, we made a more detailed study of their effect on stem elongation. Growth was measured in part by a linear transducer attached to a single segment, which resulted in a precise, continuous record of the early growth kinetics. Our results show that, in addition to inhibiting the rate of GA3-induced growth, IAA modified GA3-induced growth by shortening the response time to GA3. MATERIALS AND METHODS Plant Material. Oats (Avena sativa cv Victory) were grown in a Sherer-Gillett environmental chamber in moderately high light (50 w/m2), LD (16 h light, 8 h dark), and cool temperatures (22°C day, 16°C night). The plants were grown hydroponically in trays of vermiculite and 50% Hoagland solution. The shoots were harvested when the next-to-last internode was 1 to 3 cm long, which was about 6 to 7 weeks after planting. Segments were taken from these shoots and their growth analyzed in the following two procedures.General Growth Analysis. One-cm internodal segments with their subtending nodes were excised from oat shoots. The bottom 1 cm was cut from styrofoam cups, and holes were made in their bases to support the segments firmly by their nodes. The cup bases with the segments were then floated in Petri dishes containing 0.1 M sucrose, which is needed in long-term studies to prevent substrate limitation of growth (1). Hormone solutions were added to the interior of the internodes, which are hollow down to the nodes and thereby can serve as miniature receptacles. This anatomical feature permits the treatment solutions to be in direct contact with the growing, noncutinized cells and also permits easy exchange of solutions.Ten to fifteen segments were assembled for each treatment, and stem elongation was measured with a ruler to 0.1 mm as frequently as every 0.5 h. Solutions were added to and removed from the lacunae with a syringe or dropper to which was attached a length of polyethylene tubing (0.7 mm outside diameter). The segments were brought to osmotic equilibrium with several injections of water before the hormone solutions we...

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“…Tbe lag time required for GA3 to stimulate cell elongation is generally longer than tbe 10-15 min for auxin (Evans 1974); it is 24 min in peas (Wamer and Leopold 1971), 35-40 tnin in Avena internode segments (Adams et ai. 1973), 50 min in excised y-irradiated wheat coieoptiles (Rose 1974a), 60 min in coleoptiles from intact y-plantlets (Kern 1975), 21-90 min in coleoptile sections from dwarf com (Chang and Ruddat 1974), and less than 30 min in dwarf pea plants (McComb and Broughton 1972). A more rapid response of 10-18 min has been found in Japanese morning glorj' seedlings without roots (Baretidse et al 1978); and excised lettuce hypocotyl sections responded to GA3 in less than 15 min in 80% of experiments, though it was typically less than 10 min (Mol!…”

Section: Introductionmentioning

confidence: 99%

Gibberellic acid‐stimulated and auxin‐stimulated cell growth in relation to RNA synthesis, protein synthesis and development in the wheat coleoptile

Rose¹,

Crossman²

1982

Physiologia Plantarum

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1982. Gibberellic acid-stimulated and auxin-stimulated cell growth in relation to RNA synthesis, protein synthesis and development in the wheat coleoptile. -Physiol. Plant. 55: 395-401.Young excised coleoptiles from dark grown wheat have their cell growth promoted by gibberellic acid (GAj), while sections from older coleoptiles have their cell growth promoted by auxin. The GA3 response has a much longer lag period than that of auxin. Neither GA3 nor auxin has any effect on '*C-leucine and "C-uridine incorporation and uptake after 1 h, indicating that the lag in growth stimulation following GA3 application is not associated with changes in protein or RNA synthesis. Following a 6 h incubation there are small increases in "C-Ieucine and "C-uridine incorporation in response to both GA3 and auxin, and in the case of auxin this is associated with increased uptake. Studies on protein and RNA turnover using pulse-chase experiments have shown that both GA3 and auxin have no effect on protein and RNA stability. There are, however, developmental changes in RNA and protein synthesis that should be considered in any explanation of the mechanism of action of these hormones on cell growth. Young GA3-sensitive tissue has high rates of RNA synthesis and low protein and RNA turnover, while auxin-sensitive tissue has low rates of RNA synthesis, slightly higher rates of RNA turnover and much higher rates of protein turnover. The evidence overall favours more effective utilisation by GA3 and auxin of a basal control level of RNA and protein synthesis and turnover In coleoptile tissue.Additional key word -Triticum aestium. R. J. Rose (reprint requests) and

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Differntial effect of cycloheximide on the short term gibberellin and auxin growth kinetics of gamma-coleoptiles

Cited by 12 publications

References 18 publications

Action of Cordycepin on Gibberellic Acid Stimulated Cell Elongation in Coleoptiles from Gamma‐Plantlets of Wheat

Action of Cordycepin on Gibberellic Acid Stimulated Cell Elongation in Coleoptiles from Gamma‐Plantlets of Wheat

Interaction of Indoleacetic Acid and Gibberellic Acid in the Short-Term Growth Kinetics of Oat Stem Segments

Gibberellic acid‐stimulated and auxin‐stimulated cell growth in relation to RNA synthesis, protein synthesis and development in the wheat coleoptile

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