Differing molecular pathology of pancreatic adenocarcinoma in Egyptian and United States patients

Abstract

Variations in genetic mutations in pancreatic carcinoma between different populations have not been studied extensively, especially in developing countries where pancreatic cancer is rare. We studied the molecular pathology of 44 pancreatic carcinomas from patients residing in a heavily polluted region in the Nile River delta and compared the findings with tumors from 44 United States (US) patients. We evaluated K-ras mutations in codon 12, p53 mutations in exons 5-8, and Gadd45a mutations in exons 1 and 4. Overall, rates of K-ras, p53 and Gadd45 mutations were not statistically different in tumors of patients from Egypt and the US (67.4 vs. 63.4%; 27.3 vs. 36.4% and 9.1 vs. 4.5%, respectively). However, there were distinct differences in the specific types of K-ras and p53 mutations between the 2 groups. In K-ras, G fi T transversion mutation was more frequent in the tumors from Egypt than from the US (58.6 vs. 26.9%), whereas G fi C transversion was detected in 26.9% of US tumors but none from Egypt (p 5 0.003). We also found a trend toward differences in the p53 exons in which mutations occurred, with higher frequency of exon 5 mutation and lower frequency of exon 6 mutation in Egyptian tumors. Logistic regression showed that K-ras G fi T transversion mutations and p53 exon 6 mutations were predicted by the country of residence of the patients. Our study identifies that there are differences in the types of mutations found in tumors from pancreatic carcinoma patients in Egypt and the US, and suggests that environmental factors may explain these differences. ' 2006 Wiley-Liss, Inc.

Epidemiologic risk factors of hepatocellular carcinoma in a rural region of Egypt

“…The study questionnaire elicited the information of demographic, residential, occupational, lifestyle and medical histories as well as reproductive changes for female subjects. The questionnaire had been used in our previous studies on the epidemiology of cancer in Egypt [34][35][36]. Following the interviews, blood samples were taken from all study subjects.…”

Assessment of Pesticide Residues in Edible and Unhatched Chicken Eggs in Sharkia Province, Egypt

“…For this reason, securing the quality of hen eggs is an important issue for Chronic exposure to POPs was found to interfere with the activity of endogenous hormones in humans, including T4, T3 and TSH (Langer, 2010). Some evidence suggests that OCPs might contribute to increase the risk of exocrine pancreatic cancer (Soliman et al, 2006). It is also worth mentioning that chronic exposure to OPIs contribute to produce developmental neurotoxicity (Colborn, 2006).…”

Past medical conditions and K-ras mutations in pancreatic ductal adenocarcinoma: a hypothesis-generating study

“…Oncogenic K-ras activation can result in a wide variety of effects, from activation of a senescence program to increased cell proliferation and inhibition of apoptosis, depending on the cellular and molecular context [1,[13][14][15][16][17]. In addition, evidence from molecular pathology and epidemiology suggests that wild-type and mutated K-ras PDA may develop through pathways involving different host-gene-environment interactions [2,17,18]. There is accumulating evidencemainly from mouse models of human PDA-that acinar cells may contribute to the development of PDA; this process may be particularly relevant for patients with chronic pancreatitis [13,14].…”