Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones

Salgame, Padmini; Js, Abrams; Clayberger, Carol; Goldstein, Harris; Convit, J; Modlin, R L; Br, Bloom

doi:10.1126/science.254.5029.279

Cited by 135 publications

(30 citation statements)

References 45 publications

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“…major T-cell subsets that regulate immune cell recruitment and proliferation through a program of cytokine production. 1,2 Based on the types of secreted cytokines, CD4 T-helper cells are further subdivided into T-helper 1 (Th1), Th2, Th17 and other subsets, making cytokine profiling an important part of determining Th phenotype. Importantly, cytokines secreted by T-cells have been reported to correlate with positive or negative disease outcomes and thus provide important diagnostic information.…”

Section: Activated T-lymphocytes (T-cells) Are Central Participants O...mentioning

confidence: 99%

A microdevice for multiplexed detection of T-cell-secreted cytokines

et al. 2008

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Cytokines are produced by immune cells in response to viral or bacterial pathogens and therefore have significant diagnostic value. The goal of the present study was to develop a miniature device for detection of interleukin (IL)-2 and interferon (IFN)-gamma cytokines secreted by a small population of CD4 and CD8 T-cells. Microarrays of T-cell- and cytokine-specific Ab spots were printed onto poly(ethylene glycol) (PEG) hydrogel-coated glass slides and enclosed inside a microfluidic device, creating a miniature ( approximately 3 microL) immunoreaction chamber. Introduction of the red blood cell (RBC) depleted whole human blood into the microfluidic device followed by washing at a pre-defined shear stress resulted in isolation of pure CD4 and CD8 T-cells on their respective Ab spots. Importantly, the cells became localized next to anti-IL-2 and -IFN-gamma Ab spots. Mitogenic activation of the captured T-cells was followed by immunofluorescent staining (all steps carried out inside a microfluidic device), revealing concentration gradients of surface-bound cytokine molecules. A microarray scanner was then used to quantify the concentration of IFN-gamma and IL-2 near CD4 and CD8 T-cells. This study represents one of the first demonstrations of a microdevice for capturing desired T-cell subsets from a small blood volume and determining, on-chip, cytokine profiles of the isolated cells. Such a microdevice is envisioned as an immunology tool for multi-parametric analysis of T-cell function with direct applications in diagnosis/monitoring of HIV and other infectious diseases.

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Section: Activated T-lymphocytes (T-cells) Are Central Participants O...mentioning

confidence: 99%

A microdevice for multiplexed detection of T-cell-secreted cytokines

et al. 2008

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“…Some studies showed that ENL, like T1R, is induced primarily by a T cell-mediated immune response (15,161). CD8+ clones derived from LL lesions secrete large amounts of IL-4 and minimal IFN-γ (162,163). In response to M. leprae or M. leprae antigens, lesion-derived CD8+ T cells do not proliferate and limit the proliferation and cytokine secretion of bystander T cells (162,163).…”

Section: T Cellsmentioning

confidence: 99%

“…CD8+ clones derived from LL lesions secrete large amounts of IL-4 and minimal IFN-γ (162,163). In response to M. leprae or M. leprae antigens, lesion-derived CD8+ T cells do not proliferate and limit the proliferation and cytokine secretion of bystander T cells (162,163). Thus, CD8+ T cells may induce M. leprae-specific T cell anergy (162,163).…”

Section: T Cellsmentioning

confidence: 99%

“…In response to M. leprae or M. leprae antigens, lesion-derived CD8+ T cells do not proliferate and limit the proliferation and cytokine secretion of bystander T cells (162,163). Thus, CD8+ T cells may induce M. leprae-specific T cell anergy (162,163). In contrast, TT skin lesions display a predominance of CD4+ T cells that secrete high amounts of IFN-γ (164,165).…”

Section: T Cellsmentioning

confidence: 99%

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Host-Related Laboratory Parameters for Leprosy Reactions

et al. 2021

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Leprosy reactions are acute inflammatory episodes that complicate the course of a Mycobacterium leprae infection and are the major cause of leprosy-associated pathology. Two types of leprosy reactions with relatively distinct pathogenesis and clinical features can occur: type 1 reaction, also known as reversal reaction, and type 2 reaction, also known as erythema nodosum leprosum. These acute nerve-destructive immune exacerbations often cause irreversible disabilities and deformities, especially when diagnosis is delayed. However, there is no diagnostic test to detect or predict leprosy reactions before the onset of clinical symptoms. Identification of biomarkers for leprosy reactions, which impede the development of symptoms or correlate with early-onset, will allow precise diagnosis and timely interventions to greatly improve the patients' quality of life. Here, we review the progress of research aimed at identifying biomarkers for leprosy reactions, including its correlation with not only immunity but also genetics, transcripts, and metabolites, providing an understanding of the immune dysfunction and inflammation that underly the pathogenesis of leprosy reactions. Nevertheless, no biomarkers that can reliably predict the subsequent occurrence of leprosy reactions from non-reactional patients and distinguish type I reaction from type II have yet been found.

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“…Type 1 CD8 + T cells (Tc1) secrete IFN-γ, while type 2 CD8 + T cells (Tc2) mainly secrete IL-4 and IL-5 [94]. Although both effector cell subpopulations have been identified in human peripheral blood and in patients with various clinical disorders [95][96][97][98][99], their roles and effects on renal inflammation and fibrosis remain relatively undefined. We identified two CD8 + T cell subsets in a mouse model of UUO-induced renal fibrosis, Tc1 (CD44 + CD25 − CD62L − ) and Tc2 (CD44 + CD25 high CD62L low ), which exert diverse effects in building an inflammatory and profibrotic environment.…”

Section: The Functions Of Cd8 + T Cell Subsets Which Identified By Cd44 Cd25 and Cd62l On Myofibroblasts Accumulation In Renal Fibrosismentioning

confidence: 99%

The Mechanism of CD8+ T Cells for Reducing Myofibroblasts Accumulation during Renal Fibrosis

et al. 2021

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Renal fibrosis is a hallmark of chronic kidney disease (CKD) and a common manifestation of end-stage renal disease that is associated with multiple types of renal insults and functional loss of the kidney. Unresolved renal inflammation triggers fibrotic processes by promoting the activation and expansion of extracellular matrix-producing fibroblasts and myofibroblasts. Growing evidence now indicates that diverse T cells and macrophage subpopulations play central roles in the inflammatory microenvironment and fibrotic process. The present review aims to elucidate the role of CD8+ T cells in renal fibrosis, and identify its possible mechanisms in the inflammatory microenvironment.

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Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones

Cited by 135 publications

References 45 publications

A microdevice for multiplexed detection of T-cell-secreted cytokines

A microdevice for multiplexed detection of T-cell-secreted cytokines

Host-Related Laboratory Parameters for Leprosy Reactions

The Mechanism of CD8+ T Cells for Reducing Myofibroblasts Accumulation during Renal Fibrosis

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