Differing cytotoxicity and bioavailability of selenite, methylselenocysteine, selenomethionine, selenosugar 1 and trimethylselenonium ion and their underlying metabolic transformations in human cells

Marschall, Talke Anu; Bornhorst, Julia; Kuehnelt, Doris; Schwerdtle, Tanja

doi:10.1002/mnfr.201600422

Cited by 58 publications

(43 citation statements)

References 43 publications

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“…On the contrary, specific guidelines for single selenium species have not been unfortunately set, despite the clear evidence that the various chemical forms of selenium have different biological properties, i.e. nutritional and toxicological activities (7,9,10,62). …”

Section: Adequacy Of Environmental Standardsmentioning

confidence: 99%

Health risk assessment of environmental selenium: Emerging evidence and challenges

Vinceti

Filippini

Cilloni

et al. 2017

Molecular Medicine Reports

121

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New data have been accumulated in the scientific literature in recent years which allow a more adequate risk assessment of selenium with reference to human health. This new evidence comes from environmental studies, carried out in populations characterized by abnormally high or low selenium intakes, and from high-quality and large randomized controlled trials with selenium recently carried out in the US and in other countries. These trials have consistently shown no beneficial effect on cancer and cardiovascular risk, and have yielded indications of unexpected toxic effects of selenium exposure. Overall, these studies indicate that the minimal amount of environmental selenium which is source of risk to human health is much lower than anticipated on the basis of older studies, since toxic effects were shown at levels of intake as low as around 260 µg/day for organic selenium and around 16 µg/day for inorganic selenium. Conversely, populations with average selenium intake of less than 13–19 µg/day appear to be at risk of a severe cardiomyopathy, Keshan disease. Overall, there is the need to reconsider the selenium standards for dietary intake, drinking water, outdoor and indoor air levels, taking into account the recently discovered adverse health effects of low-dose selenium overexposure, and carefully assessing the significance of selenium-induced proteomic changes.

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Section: Adequacy Of Environmental Standardsmentioning

confidence: 99%

Health risk assessment of environmental selenium: Emerging evidence and challenges

Vinceti

Filippini

Cilloni

et al. 2017

Molecular Medicine Reports

121

View full text Add to dashboard Cite

show abstract

“…Therefore, cells were trypsinized, cell numbers and volumes were quantified, pelletized cells were digested in 20% HNO 3 using a closed microwave system (MARS 6, CEM Cooperation), diluted in deionized water and measured by ICP-MS/MS in the mass-shift mode using oxygen as reaction gas to eliminate interferences. 1 mg L À1 Rh was applied as an internal standard (further details 15,16 ).…”

Section: Validation Of Sc-icp-ms/msmentioning

confidence: 99%

Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite

et al. 2018

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Single-cell inductively coupled plasma mass spectrometry (SC-ICP-MS) has become a powerful and fast tool to evaluate the elemental composition at a single-cell level. In this study, the cellular bioavailability of arsenite (incubation of 25 and 50 μM for 0-48 h) has been successfully assessed by SC-ICP-MS/MS for the first time directly after re-suspending the cells in water. This procedure avoids the normally arising cell membrane permeabilization caused by cell fixation methods (e.g. methanol fixation). The reliability and feasibility of this SC-ICP-MS/MS approach with a limit of detection of 0.35 fg per cell was validated by conventional bulk ICP-MS/MS analysis after cell digestion and parallel measurement of sulfur and phosphorus.

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“…In agreement with observations from an acute high‐dose exposure study in C. elegans and studies in other models, for example, ref. , the seleno‐amino acids SeMet and MeSeCys accumulated to a greater extent than the inorganic selenite. Similar Se contents were obtained for the txnrd‐1 deletion mutant strain VB1414, indicating that the bioavailability of the Se species is independent of the sole C. elegans selenoprotein, which was observed before in the high‐dose study …”

Section: Resultsmentioning

confidence: 96%

Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems

Rohn

Raschke

Aschner

et al. 2019

Molecular Nutrition Food Res

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Scope: Small selenium (Se) species play a key role in Se metabolism and act as dietary sources of the essential trace element. However, they are redox-active and trigger pro-and antioxidant responses. As health outcomes are strongly species-dependent, species-specific characteristics of Se compounds are tested in vivo. Methods and results: In the model organism Caenorhabditis elegans (C. elegans), immediate and sustained effects of selenite, selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys) are studied regarding their bioavailability, incorporation into proteins, as well as modulation of the cellular redox status. While all tested Se compounds are bioavailable, only SeMet persistently accumulates and is non-specifically incorporated into proteins. However, the protection toward chemically-induced formation of reactive species is independent of the applied Se compound. Increased thioredoxin reductase (TXNRD) activity and changes in mRNA expression levels of antioxidant proteins indicate the activation of cellular defense mechanisms. However, in txnrd-1 deletion mutants, no protective effects of the Se species are observed anymore, which is also reflected by differential gene expression data. Conclusion: Se species protect against chemically-induced reactive species formation. The identified immediate and sustained systemic effects of Se species give rise to speculations on possible benefits facing subsequent periods of inadequate Se intake.

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Differing cytotoxicity and bioavailability of selenite, methylselenocysteine, selenomethionine, selenosugar 1 and trimethylselenonium ion and their underlying metabolic transformations in human cells

Cited by 58 publications

References 43 publications

Health risk assessment of environmental selenium: Emerging evidence and challenges

Health risk assessment of environmental selenium: Emerging evidence and challenges

Single-cell analysis by ICP-MS/MS as a fast tool for cellular bioavailability studies of arsenite

Treatment of Caenorhabditis elegans with Small Selenium Species Enhances Antioxidant Defense Systems

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