Differing Contribution of Polymorphonuclear Cells and Macrophages to Protection of Mice against Listeria monocytogenes and Pseudomonas aeruginosa

Tatsukawa, Keiji; Mitsuyama, Masao; Takeya, Kenji; Nomoto, Kikuo

doi:10.1099/00221287-115-1-161

Cited by 111 publications

(57 citation statements)

References 11 publications

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“…Protection against E. coli in normal mice is reported to depend mainly on PMN (23). In the present experiments, the functions of PMN in C. parvum-treated mice were not determined.…”

Section: Discussionmentioning

confidence: 58%

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Increased Susceptibility to Escherichia coli Infection in Mice Pretreated with Corynebacterium parvum

Yoshikai

Miake

Sano

et al. 1983

Microbiology and Immunology

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The contribution of activated macrophages to protection against Escherichia coli was studied in mice treated intravenously with Corynebacterium paruum 7 days before infection. C. parvum-treated mice showed increased phagocytic activity and enhanced resistance to Listeria infection. In contrast, these mice showed increased susceptibility to a subsequent challenge with E. coli that correlated closely with a reduction in the LDso of lipopolysaccharide (LPS) in these mice. The peritoneal macrophages obtained from C. parvum-treated mice had a strong ability to phagocytize and kill E. coli in in vitro experiments. A rapid decline in the number of bacteria in the liver of C. parvum-treated mice was observed in the early period ofinfection. However, the number of bacteria in liver and spleen increased progressively to a lethal dose from 6 hr after infection. At this time, a significant increase in ,a-glucuronidase, a lysosomal acid hydrolase, was found in the serum of these mice. In vitro experiments revealed that the peritoneal macrophages from C. parvum-treated mice were highly susceptible to the cytotoxic effect of LPS after 6 hr of incubation with LPS. It is suggested that the hypersensitivity of activated macrophages to the cytotoxic effect of endotoxin derived from E. coli may be partly responsible for the increased susceptibility of C. parvum-treated mice to E. coli infection.There are distinct morphological and functional differences between polymorphonuclear cells (PMN) and macrophages, although both cell types are known to play important roles in the protection against infection with a variety of bacteria (2, 6, 17). PMN are reported to be important for host resistance against Pseudomonas aeruginosa (24) and Escherichia coli (23), whereas macrophages playa protective role in the early stage of infection with Listeria monocytogenes (II).Macrophages activated by killed Corynebacterium parvum have been shown to have a high level of lysosomal enzyme activity and an increased capacity to generate oxygen radicals as much as or even more than PMN (12). However, it is not known whether activated macrophages contribute to the host resistance to E. coli infection which depends mainly on the function of PMN.In the present study, the effect of pretreatment of mice with C. parvum on E. coli infection was investigated in an attempt to determine the role of nonspecifically activated macrophages in protection against E. coli infection. Our results show an 273

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“…Protection against E. coli in normal mice is reported to depend mainly on PMN (23). In the present experiments, the functions of PMN in C. parvum-treated mice were not determined.…”

Section: Discussionmentioning

confidence: 58%

“…PMN are reported to be important for host resistance against Pseudomonas aeruginosa (24) and Escherichia coli (23), whereas macrophages playa protective role in the early stage of infection with Listeria monocytogenes (II).…”

mentioning

confidence: 99%

Increased Susceptibility to Escherichia coli Infection in Mice Pretreated with Corynebacterium parvum

Yoshikai

Miake

Sano

et al. 1983

Microbiology and Immunology

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“…Since phagocytic cells, especially PMN, are known to be responsible for the primary protection in mice against E. coli and P. aeruginosa (17,18), further experi- ments were conducted to determine whether this compound affects the functional activities of phagocytic cells. In these experiments, we used BM 12,531 as a standard agent, because it is able to increase the number of leucocytes and their phagocytic activity (4,7).…”

Section: Effects Of Isohematinic Acid and Bm 12531 On Nbt Reduction mentioning

confidence: 99%

Effects of a New Antibiotic, Isohematinic Acid, on the Resistance of Mice to Experimental Infections

Shiraishi

Katayama

Matsuda

et al. 1987

Microbiology and Immunology

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Isohematinic acid, an antibiotic newly isolated from the culture broth of Actinoplanes philippinensis SANK 61681, was assessed for its ability to enhance nonspecific resistance to bacterial infections against Escherichia coli and Pseudomonas aeruginosa in mice. This agent, as well as BM 12,531 (Azimexon), was found to prolong the survival of normal mice infected with E. coli and also of compromised mice infected with either E. coli or P. aeruginosa, whose defense system had been deteriorated by treatment with carboquone, an alkylating agent. Like BM 12,531, isohematinic acid administered to normal mice significantly increased the nitroblue tetrazolium reducing potency of polymorphonuclear leucocytes (PMN), indicating that the microbicidal activity of PMN was enhanced by these agents. In addition, in the compromised mice these agents were able to restore the number of peripheral blood leucocytes, which had been reduced to about 30% of the normal level by carboquone. These results suggest that isohematinic acid, like BM 12,531, enhances nonspecific resistance to these bacterial infections by stimulating the microbicidal activity of PMN and inducing leucocytosis.

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“…The resistance of normal mice against primary infection with P . aeruginosa seems to be attributable mainly to polymorphonuclear cells (PMN) but not to macrophages (Tatsukawa et al, 1979). On the other hand, complete Freund's adjuvant and BCG have been shown to activate mainly the functions of macrophages.…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, which was carried out to determine the effect of BCG treatment in normal mice, it was found that BCG treatment did not augment the resistance but rather enhanced a lethal effect of pseudomonas infection. In addition such BCG treatment augmented the resistance against Listeria monocytogenes to which resistance had been reported to depend mainly upon the function of macrophages (Tatsukawa et al, 1979).…”

Section: Introductionmentioning

confidence: 99%

BCG-induced Susceptibility of Mice to Challenge with Pseudomonas aeruginosa

et al. 1983

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Mice infected with Mycobacterium bovis, BCG, were shown to be highly susceptible to subsequent challenge with Pspdomonas aeruginosa. The susceptibility was characterized by the enhanced mortality and shoptened survival afteq challenge with P. aeruginosa. BCG-treated mice did not show any enhanaed susceptibility to challenge with Gram-positive bacteria such as Staphylococcus aureus or Liskria monocytogenes. BCG-treated mice eliminated P . aeruginosa from their organs in a pattqrn similar to that in untreated mice. There was no significant difference in the bactericidal activities of polymorphonuclear cells and macrophages between BCG-treated and untreated mice. An equal amount of endotoxin was detected by the Limulus lysate assay in the blood of both BCG-treated and untreated mice after challenge with P . aeruginosa. The enhanced susceptibility induced by BCG pretreatment could be decreased when the mice were rendered LPS-tolerant by injections of small amounts of LPS. These results suggest that BCG-induced slusceptibility to P . Qeruginosa can be ascribed to an enhanced susceptibility to the lethal qffect of LPS produced by challenge bacteria, and not to the impairment of the ability to eliminate infected bacteria.

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Differing Contribution of Polymorphonuclear Cells and Macrophages to Protection of Mice against Listeria monocytogenes and Pseudomonas aeruginosa

Cited by 111 publications

References 11 publications

Increased Susceptibility to Escherichia coli Infection in Mice Pretreated with Corynebacterium parvum

Increased Susceptibility to Escherichia coli Infection in Mice Pretreated with Corynebacterium parvum

Effects of a New Antibiotic, Isohematinic Acid, on the Resistance of Mice to Experimental Infections

BCG-induced Susceptibility of Mice to Challenge with Pseudomonas aeruginosa

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