Differing actions of penetrating and nonpenetrating cryoprotective agents

McGann, Locksley E.

doi:10.1016/0011-2240(78)90056-1

Cited by 160 publications

(115 citation statements)

References 24 publications

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“…39 The choice of the cryoprotective agent and its optimal concentration is important for the required cell recovery after thawing. [17][18][19] There are several HSPC cryopreservation protocols. However, only two cryoprotective agents are in current clinical use -DMSO 13,18,20 and/or hydroxyethyl starch (HES), 31 but in various concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…Dimethyl sulfoxide (DMSO) has been commonly used as a cryoprotectant in different (2.2-10%) concentrations. 9,[17][18][19][20] Finally, a higher degree of cell destruction has been observed when the transition period from liquid to solid phase (release of the fusion heat) was prolonged. 9,21 Although HSPC cryopreservation procedures are already in routine use, some problems related to the optimal cooling velocity during controlled-rate freezing and the choice of the cryoprotective agent at the appropriate concentration are still unresolved.…”

mentioning

confidence: 99%

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The cryopreservation protocol optimal for progenitor recovery is not optimal for preservation of marrow repopulating ability

Balint

Ivanović

Petakov

et al. 1999

Bone Marrow Transplant

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Summary:The efficiency of five different cryopreservation protocols (our original controlled-rate and noncontrolled-rate protocols) was evaluated on the basis of the recovery after thawing of very primitive pluripotent hemopoietic stem cells (MRA CFU-GM ), pluripotent progenitors (CFUSd12) and committed granulocyte-monocyte progenitors (CFU-GM) in mouse bone marrow. Although the nucleated cell recovery and viability determined immediately after the thawing and washing of the cells were found to be similar, whether controlled-rate or noncontrolled-rate cryopreservation protocols were used, the recovery of MRA CFU-GM , CFU-Sd12 and CFU-GM varied depending on the type of protocol and the cryoprotector (DMSO) concentrations used. It was shown that the controlled-rate protocol was more efficient, enabling better MRA CFU-GM , CFU-Sd12 and CFU-GM recovery from frozen samples. The most efficient was the controlled-rate protocol of cryopreservation designed to compensate for the release of fusion heat, which enabled a better survival of CFU-Sd12 and CFU-GM when combined with a lower (5%) DMSO concentration. On the contrary, a satisfactory survival rate of very primitive stem cells (MRA CFU-GM ) was achieved only when 10% DMSO was included with a fivestep protocol of cryopreservation. These results point to adequately used controlled-rate freezing as essential for a highly efficient cryopreservation of some of the categories of hematopoietic stem and progenitor cells. At the same time, it was obvious that a higher DMSO concentration was necessary for the cryopreservation of very primitive stem cells, but not, however, for more mature progenitor cells (CFU-S, CFU-GM). These results imply the existence of a mechanism that decreases the intracellular concentration of DMSO in primitive MRA cells, which is not the case for less primitive progenitors.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The cryopreservation protocol optimal for progenitor recovery is not optimal for preservation of marrow repopulating ability

Balint

Ivanović

Petakov

et al. 1999

Bone Marrow Transplant

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show abstract

“…Based on the present knowledge, CPAs lower the freezing point and replace some of the bound water molecules in and around intracellular components [19]. The CPAs also stabilize cell proteins and the plasma membrane [21], to reduce the intracellular concentration of electrolytes [22].…”

Section: Introductionmentioning

confidence: 99%

Whole sheep ovary cryopreservation: evaluation of a slow freezing protocol with dimethylsulphoxide

Milenković

Wallin

Ghahremani

et al. 2010

J Assist Reprod Genet

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“…The development of an effective, safe, and sterile cryopreservation protocol is a prerequisite for the long-term storage of the cell-assembly and organ manufacturing products with respect to the serious donor shortage of organs and laborintensive cultures. 6 One of the vital issues is to develop an effective and suitable cooling/warming procedure for the cell containing constructs [5]. In our preliminary study, a 78.7% cell viability was obtained with the incorporation of DMSO in the gelatin/alginate/fibrinogen hydrogel [2].…”

Section: Introductionmentioning

confidence: 88%

“…Protection 7 against intracellular ice formation has been attributed to the colligative effects of cryoprotectant chemicals [6]. Careful component selection is another vital issue to avoid toxic effects of the cryoprotectant, as well as the prevention of ice formation during cryopreservation [7].…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of cell/hydrogel constructs based on a new cell-assembling technique

Wang

Yan

et al. 2009

Nature Precedings

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Organ manufacturing products hold the promise to be used widely in the future for complex organ failings. The cryopreservation of the product is a very important step in the commercialization activities. In this article, a new cell cryopreservation technique, whereby, cryoprotectants were directly incorporated into the cell/hydrogel constructs, prototyped according to the predesigned structure and then subjected to a special freezing/thawing process. The rheological and hydration properties of the cryopreservation systems indicated that the hydratabilities of the gelatin/alginate hydrogels were greatly increased while the eutectic temperatures were greatly decreased by the addition of glycerol. Dextran-40 was found to be effective to improve the cell survival when incorporated with glycerol. The optimal volume concentration of the

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Differing actions of penetrating and nonpenetrating cryoprotective agents

Cited by 160 publications

References 24 publications

The cryopreservation protocol optimal for progenitor recovery is not optimal for preservation of marrow repopulating ability

The cryopreservation protocol optimal for progenitor recovery is not optimal for preservation of marrow repopulating ability

Whole sheep ovary cryopreservation: evaluation of a slow freezing protocol with dimethylsulphoxide

Cryopreservation of cell/hydrogel constructs based on a new cell-assembling technique

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