Differenzierung des Erdnußstauche-Virus (peanut stunt virus) in 6 Serotypen

Richter, Steffi; Haack, Ilse; Wesemann, Biol.-Labor. Monika; Beczner, L.

doi:10.1080/03235408709438061

Cited by 10 publications

(4 citation statements)

References 3 publications

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“…Subgroup I was exemplified by isolates from the Eastern (E) serotype, and subgroup II by isolates of the Western (W) serotype (Mink et al, 1969). These analyses were mostly carried out with isolates from the USA, but work with European isolates suggested a greater diversity of PSV (Richter et al, 1987). Our results show that six out of seven PSV isolates compared fit into the bimodal classification scheme : three isolates belong to subgroup I (F352, 1339 and 1507) and three to subgroup II (isolates B, Su and W) based on comparisons of their CPs and Northern blot hybridization.…”

Section: Discussionmentioning

confidence: 81%

“…RoMV was unequivocally classified as a subgroup I isolate by our CP analysis. This was not the case, however, when immunodiffusion was used as the comparison procedure (Richter et al, 1987). This minor discrepancy may be easily explained by the different analytical methods used.…”

Section: Discussionmentioning

confidence: 96%

“…The exploration of the third factor, the sat RNA itself, has been limited up until now to a small number of sequence variants. It is interesting to note that PSV sat RNAs have been reported only from subgroup I strains, either from Eastern USA (Collmer et al, 1985 ;Naidu et al, 1991) or from Eastern Europe (Ferreiro et al, 1996 ;Richter et al, 1987), and that all are extremely similar in sequence, a situation that clearly differs from that of CMV sat RNAs (Fraile & Garcı! a-Arenal, 1991).…”

Section: Discussionmentioning

confidence: 99%

“…az-Ruiz & Kaper, 1983) and, recently, by nucleotide sequence analysis of RNA 3, which showed identities of above 90 % for subgroup I (E-type) strains and of about 80 % between subgroup I and subgroup II (W-type) strains (Hu et al, 1997). Nevertheless, serology comparisons involving primarily Old World isolates suggest a higher diversity for PSV, as there are strains that do not fit clearly into types E (subgroup I) or W (subgroup II) (Richter et al, 1987). This was especially the case for isolates originally classified as robinia mosaic virus (RoMV ; Schmelzer, 1971).…”

Section: Introductionmentioning

confidence: 99%

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Differential interactions among isolates of peanut stunt cucumovirus and its satellite RNA.

Militao

Moreno

Rodríguez‐Cerezo

et al. 1998

Journal of General Virology

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The interactions of seven isolates of peanut stunt cucumovirus (PSV) originating from North America, Europe and Africa, and two variants of PSV satellite RNA (sat RNA) were analysed. Electrophoretic and immunoblot analyses of the coat protein (CP) and Northern blot hybridization analyses of the viral RNAs showed that isolates PSV F352, 1339 and 1507 belonged to subgroup I, and isolates PSV W, Su and B to subgroup II. The seventh isolate, robinia mosaic virus (RoMV) clustered with subgroup I isolates by CP analysis, but was related to both subgroups by RNA hybridization analysis. The ability to support the accumulation of two newly described sat RNA variants, P4 and P6 sat RNAs, was not

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential interactions among isolates of peanut stunt cucumovirus and its satellite RNA.

Militao

Moreno

Rodríguez‐Cerezo

et al. 1998

Journal of General Virology

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Nucleotide sequence analysis of peanut stunt virus Rp strain suggests the role of homologous recombination in cucumovirus evolution

et al. 2008

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The complete nucleotide (nt) sequence of peanut stunt virus Robinia strain (PSV-Rp) was determined and compared to other PSV strains and to representatives of the genus Cucumovirus. Nt sequence comparison showed 74.1-84.6% identity with the known PSV strains. Phylogenetic analysis revealed the different origin of the two genes encoded by RNA3. While the 3a gene clustered with PSV-W, the coat protein gene clustered with PSV-Mi. Recombination breakpoint analysis revealed two recombination points on RNA3. Based on these results, the establishment of a fourth PSV subgroup is proposed. This work revealed that homologous recombination occurred during the evolution of PSV.

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Characterisation of black locust isolates of Peanut stunt virus (PSV) from the Pannon ecoregion show the frequent occurrence of the fourth taxonomic PSV subgroup

2009

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Biological and molecular characterisation of ten Peanut stunt virus (PSV) isolates from Robinia pseudoacacia was carried out. The host range of these isolates was similar to that of the previously described PSV strains in most cases, but on Pisum sativum and Lens culinaris latent infection was induced. Variability in systemic symptoms was observed only on Nicotiana glutinosa. The partial RNA3 sequences were determined, including the carboxyl terminal region of the movement protein gene, the intergenic region, the entire coat protein gene and the 3′ untranslated region. Nucleotide sequence comparison of the coat protein genes showed 77.6-84.2% identity with most of the known PSV strains and 96.3-98.0% identity with PSV-Rp the typical member of subgroup IV. Phylogenetic analysis indicated the presence of the ancient homologous recombination in all of the examined black locust isolates and all the isolates were members of the fourth PSV subgroup. These results showed that the isolates of the fourth subgroup are widely distributed in black locust in this region.Peanut stunt virus (PSV) is an economically important pathogen of legumes worldwide, causing severe symptoms on alfalfa (Medicago sativa), bean (Phaseolus vulgaris), pea (Pisum sativum), peanut (Arachis hypogaea), various types of clover (Trifolium spp.) and black locust (Robinia pseudoacacia) all over the world (Mink 1972) except Australia. Disease surveys revealed that black locust trees, a well established tree in central Europe, are widely infected by PSV and could act as a primary virus source for legumes and other susceptible plants.PSV is a member of the genus Cucumovirus in the family Bromoviridae. Other members of the genus are Tomato aspermy virus (TAV) and the type member Cucumber mosaic virus (CMV) (Fauquet and Mayo 2001). The genome of PSV consists of three genomic RNAs with positive polarity, designated RNA1, RNA2 and RNA3, in order of decreasing size. RNA1 and RNA2 code for the viral components of the replicase complex. RNA2 also codes for a small protein called 2b, which is responsible for the suppression of posttranscriptional gene silencing and also functions in host-specific long-distance movement (Netsu et al., Eur J Plant Pathol (2009) 125:671-677

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Differenzierung des Erdnußstauche-Virus (peanut stunt virus) in 6 Serotypen

Cited by 10 publications

References 3 publications

Differential interactions among isolates of peanut stunt cucumovirus and its satellite RNA.

Differential interactions among isolates of peanut stunt cucumovirus and its satellite RNA.

Nucleotide sequence analysis of peanut stunt virus Rp strain suggests the role of homologous recombination in cucumovirus evolution

Characterisation of black locust isolates of Peanut stunt virus (PSV) from the Pannon ecoregion show the frequent occurrence of the fourth taxonomic PSV subgroup

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