Differentiation-specific localization of catalase and hydrogen peroxide, and their alterations in rat skin exposed to ultraviolet B rays

Muramatsu, Shigenori; Suga, Yasushi; Mizuno, Yuki; Hasegawa, Toshio; Matsuba, Shoichi; Hashimoto, Yukiko; Völkl, Alfred; Seitz, Jürgen; Ogawa, Hideoki

doi:10.1016/j.jdermsci.2004.11.001

Cited by 26 publications

(16 citation statements)

References 26 publications

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“…For example, CAT activities of diVerentiated KC were higher than those of undiVerentiated KC of the basal layer, showing that the calcium-induced diVerentiated KC had approximately 200% higher activity compared to CAT activity of un-diVerentiated KC [20]. Consistently, major CAT expression was detected from the diVerentiated KC of granular and horny layers [15]. Cu/Zn-SOD activity was much higher in the calcium-induced diVerentiated KC than in undiVerentiated KC [21].…”

Section: Discussionmentioning

confidence: 71%

“…The ubiquitous expression and active induction of phase 2 enzymes during KC diVerentiation confer an eYcient defense system together with the classic antioxidant system in the skin. Accumulation of studies demonstrates that the classical antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), play an important role in protecting the skin from oxidative damage during epidermal diVerentiation [2,15,20,21]. For example, CAT activities of diVerentiated KC were higher than those of undiVerentiated KC of the basal layer, showing that the calcium-induced diVerentiated KC had approximately 200% higher activity compared to CAT activity of un-diVerentiated KC [20].…”

Section: Discussionmentioning

confidence: 99%

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Nrf2-dependent and Nrf2-independent induction of phase 2 detoxifying and antioxidant enzymes during keratinocyte differentiation

et al. 2012

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As antioxidant enzymes can be actively modulated during keratinocyte (KC) differentiation, this study was aimed to evaluate the modulation of a group of phase 2 detoxifying and antioxidant enzymes (phase 2 enzymes) during KC differentiation. In postconfluence-induced differentiation model of KC, heme oxygenase-1 (HO-1), NADP(H):quinone oxidoreductase-1 (NQO-1), and glutathione S-transferase pi (GSTpi) were up-regulated at a transcriptional level. In Western blot analysis, the phase 2 enzymes were up-regulated by H(2)O(2), but down-regulated by N-acetyl cysteine, indicating the active role of reactive oxygen species for their expression during KC differentiation. When a redox-sensitive NF-E2 related factor-2 (Nrf2), a key transcriptional factor for phase 2 enzymes, was knocked down by small interfering RNA transfection in differentiated KCs, only NQO-1 was down-regulated in both mRNA and protein levels. In human skin, expression levels of the phase 2 enzymes were up-regulated in the differentiated KC in the normal epidermis and keratotic foci in squamous cell carcinoma, further supporting the differentiation-dependent expression of phase 2 enzymes in vivo. This study demonstrates that a group of phase 2 enzymes are modulated during KC differentiation via either Nrf2-dependent (NQO-1) or Nrf2-independent (HO-1 and GSTpi) ways.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Nrf2-dependent and Nrf2-independent induction of phase 2 detoxifying and antioxidant enzymes during keratinocyte differentiation

et al. 2012

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“…The latter was further supported by observation of correlation between ROS concentration and degree of keratinocyte differentiation (41,42). We therefore hypothesized that eTRPM8-dependent production of both ATP and O…”

Section: Suppresses Omentioning

confidence: 65%

Epidermal TRPM8 channel isoform controls the balance between keratinocyte proliferation and differentiation in a cold-dependent manner

Bidaux

Borowiec

Gordienko

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Deviation of the ambient temperature is one of the most ubiquitous stimuli that continuously affect mammals' skin. Although the role of the warmth receptors in epidermal homeostasis (EH) was elucidated in recent years, the mystery of the keratinocyte mild-cold sensor remains unsolved. Here we report the cloning and characterization of a new functional epidermal isoform of the transient receptor potential M8 (TRPM8) mild-cold receptor, dubbed epidermal TRPM8 (eTRPM8), which is localized in the keratinocyte endoplasmic reticulum membrane and controls mitochondrial Ca 2+ concentration ([Ca 2+ ] m ). In turn, [Ca 2+ ] m modulates ATP and superoxide (O •−2 ) synthesis in a cold-dependent manner. We report that this fine tuning of ATP and O •−2 levels by cooling controls the balance between keratinocyte proliferation and differentiation. Finally, to ascertain eTRPM8's role in EH in vivo we developed a new functional knockout mouse strain by deleting the pore domain of TRPM8 and demonstrated that eTRPM8 knockout impairs adaptation of the epidermis to low temperatures.he skin epidermis provides a protective barrier that guards the body against an uncongenial environment. Under the influence of a variety of ambient factors the skin epidermis undergoes continuous regeneration through so-called epidermal homeostasis (EH): the fine-tuning of the balance between proliferation, directional migration, differentiation, and death of keratinocytes. EH involves complex molecular and chemical pathways, regulating dynamic and continuous transition of keratinocytes from the proliferating state in the basal layer to the nonproliferating state in the suprabasal layer before the beginning of the differentiation in the stratum spinosum and stratum granulosum. The terminal differentiation step, characterized by keratinocyte death, transforms keratinocytes into corneocytes, which form the waterproof, mechanically resistant sheath of the stratum corneum (1).Deviation of the ambient temperature is one of the most important stimuli that constantly affect mammals' skin. At ambient temperatures from +10°C to +30°C, the unprotected human skin temperature settles at mean steady-state values within the range of +24°C to +33°C, respectively (2). Temperature is perceived by thermoreceptors, the ion channels that belong to the transient receptor potential (TRP) superfamily (for review see ref.3). Of these, TRPV1 and TRPV2 are activated by heat (above 42°C and above 52°C, respectively) (4), whereas TRPM8 and likely TRPA1 are activated by mild (5, 6) and noxious (7-9) cold, respectively. Heatstimulated keratinocytes have been shown to secrete ATP (10) and, taking into account that purinergic receptors are expressed in keratinocytes (11), TRPV3 is involved in a paracrine heat-

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“…It is well known that after UVB irradiation, H 2 O 2 accumulates in the skin via: (i) reduction in CAT activity [4,11,17,39,40] or (ii) rapid release of O 2- [6,9,41], which is dismutated to H 2 O 2 under physiological conditions [42]. Currently, genistein is being related to reduce H 2 O 2 generation in the skin, which are thought to be the main mechanisms underlying prevention of UVB radiation-induced lesions.…”

Section: Discussionmentioning

confidence: 99%

Genistein prevents ultraviolet B radiation-induced nitrosative skin injury and promotes cell proliferation

Terra

Souza-Neto

Frade

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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Differentiation-specific localization of catalase and hydrogen peroxide, and their alterations in rat skin exposed to ultraviolet B rays

Cited by 26 publications

References 26 publications

Nrf2-dependent and Nrf2-independent induction of phase 2 detoxifying and antioxidant enzymes during keratinocyte differentiation

Nrf2-dependent and Nrf2-independent induction of phase 2 detoxifying and antioxidant enzymes during keratinocyte differentiation

Epidermal TRPM8 channel isoform controls the balance between keratinocyte proliferation and differentiation in a cold-dependent manner

Genistein prevents ultraviolet B radiation-induced nitrosative skin injury and promotes cell proliferation

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