Differentiation potential of urothelium from patients with benign bladder dysfunction

Abstract

LKT and IE contributed equally assessed by the acquisition of a transitional cell morphology, a switch from a cytokeratin (CK)13 lo /CK14hi to a CK13 hi /CK14 lo phenotype, expression of claudin 3, 4 and 5 proteins, and induction of uroplakin gene transcription. RESULTSTwo of 12 SUI cell lines showed early senescent changes in culture and were not characterized further; one of seven IC, one of five IDO and a further three SUI cell lines had some evidence of senescence at passage 3. Of the seven IC-derived cell lines, four showed a near normal range of differentiationassociated responses, but the remainder showed little or no response. Most IDO cell lines (four of five) showed a normal differentiation response, but at least three of the 10 SUI cell lines showed some compromise of differentiation potential. CONCLUSIONThis study supports the existence of a subset of patients with IC in whom a failure of urothelial cytodifferentiation might contribute to the disease, and provides a novel platform for investigating the cell biology of urothelium from SUI and other benign dysfunctional conditions.

FOXA1 and IRF-1 intermediary transcriptional regulators of PPARγ-induced urothelial cytodifferentiation

“…38 The reactions were performed in TaqMan Universal PCR master mix with 300 nM of primers and 200 nM of probe on an ABI Prism 7700 Sequence Detector system (from Applied Biosystems) for 10 min at 951C followed by 40 cycles of 15 s at 951C and 60 s at 601C. The data was analysed using the ABI Prism 7700 SDS software.…”

Krüppel-like Factor 4 Promotes Esophageal Squamous Cell Carcinoma Differentiation by Up-regulating Keratin 13 Expression

“…KRT13, one of the type I KRT, is often paired with KRT4, which expresses in suprabasal layers of noncornified and stratified squamous epithelium (13). Furthermore, during the urothelial differentiation, a switch from a KRT13 (low)/KRT14 (high) to a KRT13 (high)/KRT14 (low) phenotype is observed when a transitional cell morphology was acquired (14). Alternatively, heterozygous missense mutation of mucosal KRT13 results in white sponge nevus, an autosomal dominant inherited form of mucosal leukokeratosis (15).…”

Sudan kaçınma stresi uygulanmış erkek sıçan mesanesinde oksitosin etkisi: Işık ve elektron mikroskobik inceleme

“…Ürotelyal disfonksiyon, İS etyopatogenezindeki önemli unsurlardan birisidir (5,7). Mesane epitelini örten GAG tabakası ve hücreler arasındaki sıkı bağlantılar idrar içerisinde bulunan uyarıcı maddelere karşı bir bariyer görevi görmekte ve bu alanlarda oluşan hasar hastalığın patolojisinde önemli rol oynamaktadır (9,29,30). Protamin sülfat uygulanarak İS oluşturulan dişi sıçanlara ait mesane dokusunun TEM düzeyindeki incelemelerinde, GAG tabakasında düzensizlik ve sıkı bağlantılarda görülen genişlemeler RR boyaması yapılarak saptanmıştır (31).…”