Differentiation of yeast‐like fungi using the commercial Auxacolor system*

Abstract

Technical factors preclude the use of conventional fermentation and assimilation procedures for the routine laboratory differentiation of yeast-like fungal isolates. Commercial biochemical differentiation systems are therefore used to remedy this situation. The predictive accuracy of three commercially available differentiation systems was tested using 120 yeast-like fungal isolates. The morphological characteristics of these fungal strains were identical in the case of all three differentiation systems. The fungal isolates were correctly identified in 98.3% of cases with Auxacolor, in 90.8% of cases with API 20C AUX and in 76.7% of cases with the Mycotube system. The advantages and disadvantages of these biochemical differentiation systems are discussed.

Evaluation of the Auxacolor System for Biochemical Identification of Medically Important Yeasts

“…The overall rate of correct identification of 91.4% found in this study for the AUX was in line with previously documented rates of 98.3 and 85.7% (1,2) and was superior to that of the API 20C. This superiority was preserved in the species-byspecies analysis with the exception of Cryptococcus albidus (four of four isolates correctly identified by the API 20C versus three of four with the AUX).…”

Comparison of Auxacolor with API 20 C Aux in yeast identification

“…We found that, without supplementary tests, Auxacolor and API 20 C Aux gave correct identification in 69.4% and 60.2% of cases, respectively. In three other studies, Auxacolor correctly identified yeast species in 98.3%, 85.7% and 77.4% of cases, respectively [10–12].…”

“…With Auxacolor, the rates of correct identification of 60.1% (24 h), 69.4% (48 h) and 69.9% (72 h) were inferior to the correct identification rates reported in the literature [8–12]. Dupont [9] examined 227 yeast strains with API 20 C Aux and Auxacolor.…”

An overview of the vaccine platforms to combat COVID-19 with a focus on the subunit vaccines

“…On the contrary, HLAB*15:03 has the greatest capacity to present highly conserved SARS-CoV-2 peptides shared among common human coronaviruses, and it could potentially induce cross-protective T-cell immunity ( Nguyen et al, 2020 ). Association of many other susceptibility markers with SARS-CoV-2 infection like ABO blood antigens, ACE2 deletion/insertion (D/I) polymorphisms, a protective polymorphism (rs35705950-T) in Mucin 5B gene, and loss-of-function mutations in the X-chromosomal TLR7 gene, to name just a few, have been considered in many other studies ( Adli et al, 2022 ; Delanghe et al, 2020 ; Hantschke, 1996 ). The diversity and heterogeneity of the immune response to vaccines is still a potential issue in providing vaccines to the public ( Poland et al, 2007 ) .…”