Differentiation of viable and non-viable bacterial biofilms using electrorotation

Zhou, Xiaofeng; Markx, Gerard H.; Pethig, Ronald; Eastwood, Ian M.

doi:10.1016/0304-4165(95)00072-j

Cited by 69 publications

(48 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The potential across the medium, and the magnitude of the electric field experienced by the particle is then also frequency dependent and the observed electrokinetic forces on the particles will also be affected. This has been discussed in the ac electrokinetic literature and used to correct the measured force on particles at low frequencies [45].…”

Section: Electric Field In An Electrolytementioning

confidence: 99%

Ac electrokinetics: a survey of sub-micrometre particle dynamics

Green

Ramos

Morgan

2000

J. Phys. D: Appl. Phys.

210

160

View full text Add to dashboard Cite

Abstract. Particles suspended in fluid exhibit motion when subjected to ac electric fields. The applied field results in forces on both the particles and the fluid, the study of which is referred to as ac electrokinetics. The ac electrokinetic techniques can be used for the controlled manipulation and characterization of particles, and the separation of mixtures. For sub-micrometre particles, Brownian motion is important and strong electric fields are required to overcome these effects. Planar micro-electrode arrays, fabricated using semiconductor manufacturing processes, can generate electric fields of the required strength from low potentials over a wide range of frequencies. This paper reviews and discusses sub-micrometre particle dynamics under the influence of dielectrophoretic and electrohydrodynamic forces. New experimental observations of the movement of sub-micrometre particles are also presented.

show abstract

Section: Electric Field In An Electrolytementioning

confidence: 99%

Ac electrokinetics: a survey of sub-micrometre particle dynamics

Green

Ramos

Morgan

2000

J. Phys. D: Appl. Phys.

210

160

View full text Add to dashboard Cite

show abstract

“…5 shows a theoretical plot of the relative force on a particle as a function of frequency and medium conductivity. The two dispersions represented were calculated using the method outlined in Zhou et al [18] following the work of O'Brien [19]. It can be seen that as the conductivity of the medium increases, the crossover frequency decreases and the relative magnitudes of the forces change.…”

mentioning

confidence: 99%

Manipulation and trapping of sub-micron bioparticles using dielectrophoresis

Green

Morgan

Milner

1997

Journal of Biochemical and Biophysical Methods

159

127

View full text Add to dashboard Cite

A non-uniform alternating electric field induces motion in polarisable particles called dielectrophoresis. The effect is governed by the relative magnitudes of the dielectric properties of the medium and the particles. The technology has been used to manipulate particles for biotechnological applications, including purification, fractionation and concentration of cells and microorganisms. However, the lower size limit for the dielectrophoretic manipulation of particles was believed to be about 1 m, but recent work has proved otherwise. The dielectrophoretic movement and properties of latex beads and a simple rod-shaped virus, tobacco mosaic virus (TMV), have been measured using microfabricated electrode structures. Measurements have been made over a range of suspending medium conductivities, applied frequencies and electric field strengths. It is shown that under appropriate conditions both latex beads and tobacco mosaic virus particles can be selectively attracted to regions of high electric field strength located at the tips of microfabricated electrode structures. The ability to selectively trap and separate bio-particles has many potential applications in the area of biotechnology. © 1997 Elsevier Science B. V.

show abstract

“…Moreover, this selective sensitivity can be tuned by adjusting the AC frequency and the conductivity of the solution. [6][7][8] For example, live and dead bacteria have been shown to migrate by DEP forces to different regions on the chip at the same frequency because of the change in their ion channel activity. DEP separation by size and shape of the bacteria can be adjusted by altering the condition of the medium or by forming conductivity gradients.…”

mentioning

confidence: 99%

An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting

2007

View full text Add to dashboard Cite

Multi-target pathogen detection using heterogeneous medical samples require continuous filtering, sorting, and trapping of debris, bioparticles, and immunocolloids within a diagnostic chip. We present an integrated AC dielectrophoretic ͑DEP͒ microfluidic platform based on planar electrodes that form three-dimensional ͑3D͒ DEP gates. This platform can continuously perform these tasks with a throughput of 3 L / min. Mixtures of latex particles, Escherichia coli Nissle, Lactobacillus, and Candida albicans are sorted and concentrated by these 3D DEP gates. Surface enhanced Raman scattering is used as an on-chip detection method on the concentrated bacteria. A processing rate of 500 bacteria was estimated when 100 l of a heterogeneous colony of 10 7 colony forming units /ml was processed in a single pass within 30 min.

show abstract

Differentiation of viable and non-viable bacterial biofilms using electrorotation

Cited by 69 publications

References 30 publications

Ac electrokinetics: a survey of sub-micrometre particle dynamics

Ac electrokinetics: a survey of sub-micrometre particle dynamics

Manipulation and trapping of sub-micron bioparticles using dielectrophoresis

An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting

Contact Info

Product

Resources

About