Differentiation of transplanted mesenchymal stem cells in a large osteochondral defect in rabbit

Tatebe, Masahiro; Nakamura, Ryota; Kagami, Hideaki; Okada, K.; Ueda, Minoru

doi:10.1080/14653240500361350

Cited by 75 publications

(60 citation statements)

References 22 publications

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“…Alternative regenerative approaches, regarding cartilage tissue engineering, are being developed, in order to overcome these disadvantages. Mosaicplasty is characterized by the transplantation of various small autologous osteochondral grafts to the injured joint site [13]. This procedure, however, is not suitable for OA patients or suffering from rheumatoid arthritis (RA), as chondrocytes in these patients have different biological properties [14].…”

Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

“…RA is a systemic autoimmune disease, characterized by a continuous inflammation phenomenon, a result of an intrinsic imbalance, culminating in a major synovial hyperplasia, bone, and cartilage damage [15]. Treatments involving artificial prosthesis are quite invasive and lifetime limited [9], as well as the mosaicplasty treatment technique is invasive and causes damages to the donor site [13] and fails to restore functional, as well as, phenotypically stable hyaline cartilage [4].…”

Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

“…Chondrocytes are expanded in vitro in a monolayer culture and then implanted in the lesion site. Despite the small amounts of donor cartilage used, it is necessary to minimize the invasiveness of the technique [13]. During the in vitro expansion period, many chondrocytes dedifferentiate and become unsuited to produce stable hyaline cartilage, thus impacting the final clinical outcome [4,18].…”

Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

“…The unique matrix structure, rich in collagen fibers, proteoglycans, and interstitial fiber, provides a viscoelastic environment that allows the AC to support its biomechanical functions [8]. It has been well established that the AC has limited self-healing capacities [3][4][5][7][8][9][10][11][12][13][14][15] due to its intrinsic characteristics, namely its avascular nature, limited number of resident stem cells, and unique matrix organization [4]. Partial defects on mature cartilage do not heal spontaneously.…”

Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

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Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

Branquinho¹,

Caseiro²,

SantosPedrosa³

et al. 2018

Tissue Regeneration

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Musculoskeletal injuries impact millions of people globally and affect their health and well-being as well as of their companion and athletic animals. Soft-tissue injuries represent almost half of these and are associated with unorganized scar tissue formation and long time-depending healing processes. Cell-based therapeutic strategies have been developed in the past decades aiming at the treatment and reversion of such disorders. Stem cells are fairly appealing in the field, being a responsive undifferentiated population, with ability to self-renew and differentiate into different lineages. Mesenchymal stem cells (MSCs) can be obtained from several adult tissues, including the synovial membrane. Synovia-derived MSCs can be found in individuals of any age and are associated to intrinsic regenerative processes, through both paracrine and cell-to-cell interactions, thus, contributing to hosts' healing capacity. Studies have demonstrated the potential benefit of synovia-derived MSCs in these regenerative processes in both human and veterinary medicine. The purpose of this chapter is to review the literature regarding SM-MSC therapies applied to musculoskeletal disorders, in both human and veterinary medicine.

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Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

Section: Osteoarticular Disorders and Regeneration Physiologymentioning

confidence: 99%

See 2 more Smart Citations

Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

Branquinho¹,

Caseiro²,

SantosPedrosa³

et al. 2018

Tissue Regeneration

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“…According to the manufacturer, PKH26 dye remains stable in the cells for up to 100 days in vivo, and is therefore widely used in cell-tracing experiments [25,26]. We followed the PKH26 kit manufacturer's recommendations for the optimization of the staining procedure.…”

Section: Staining Fetal Cells With Pkh26 Fluorescent Dyementioning

confidence: 99%

Engrafting fetal liver cells into multiple tissues of healthy adult mice without the use of immunosuppressants

Darinskas

Gasparavičiūtė

Mališauskas

et al. 2007

Cellular and Molecular Biology Letters

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Abstract:We have shown the fetal liver cell engraftments into multiple tissues of adult healthy mice, achieved without suppressing the animals' immune systems. Fetal cells from the livers of male C57Bl/6J Black lineage mice at day 13 to 15 of gestation were injected intravenously into female adult CC57W/MY White mice. The grafting was evaluated by Y-chromosome-specific PCR, cytometric analysis of fluorescently stained donor cells, and histological analysis. All the methods consistently showed the presence of multiple engraftments randomly distributed through the various organs of the recipients. After 60 days, the grafts still constituted 0.1 to 2.75% of the tissues. The grafted cells did not change their appearance in any of the organs except the brain, where they became enlarged. Inflammatory reactions were not detected in any of the histological preparations. The frequency of engraftments was higher in the liver, indicating that similarity between the donor and recipient cells facilitates engraftment. The high inherent plasticity of fetal liver cells underlies their ability to integrate into healthy recipient organs, which can be governed by environmental conditions and connections with neighboring cells rather than by the initial cellular developmental programs. The fact that fetal liver cells can be grafted into multiple tissues of healthy animals indicates that they can be used to replace the natural loss of cells in adult organisms.

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The therapeutic applications of multipotential mesenchymal/stromal stem cells in skeletal tissue repair

Arthur

Zannettino

Gronthos

2008

Journal Cellular Physiology

289

179

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Four decades after the first isolation and characterization of clonogenic bone marrow stromal cells or mesenchymal stem cells (MSC) in the laboratory of Dr. Alexander Friedenstien, the therapeutic application of their progeny following ex vivo expansion are only now starting to be realized in the clinic. The multipotency, paracrine effects, and immune-modulatory properties of MSC present them as an ideal stem cell candidate for tissue engineering and regenerative medicine. In recent years it has come to light that MSC encompass plasticity that extends beyond the conventional bone, adipose, cartilage, and other skeletal structures, and has expanded to the differentiation of liver, kidney, muscle, skin, neural, and cardiac cell lineages. This review will specifically focus on the skeletal regenerative capacity of bone marrow derived MSC alone or in combination with growth factors, biocompatible scaffolds, and following genetic modification.

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Differentiation of transplanted mesenchymal stem cells in a large osteochondral defect in rabbit

Cited by 75 publications

References 22 publications

Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

Synovia-Derived Mesenchymal Stem Cell Application in Musculoskeletal Injuries: A Review

Engrafting fetal liver cells into multiple tissues of healthy adult mice without the use of immunosuppressants

The therapeutic applications of multipotential mesenchymal/stromal stem cells in skeletal tissue repair

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