Differentiation of the Dragonfly Genus Davidius (Odonata: Gomphidae) in Japan Inferred from Mitochondrial and Nuclear Gene Genealogies

Abstract

To infer the differentiation of Japanese Davidius dragonflies, we investigated the genealogies of the mitochondrial cytochrome oxidase subunit I gene (COI) and the nuclear ribosomal RNA gene region encompassing 18S, ITS1, 5.8S, and ITS2 sequences for three species endemic to Japan--Davidius nanus, D. fujiama, and D. moiwanus--as well as D. lunatus from the Korean Peninsula. According to the mitochondrial and nuclear gene genealogies, D. nanus and D. moiwanus are closely related and are sister to the continental species D. lunatus, whereas D. fujiama differentiated from an ancestor of the other three species. Although the mitochondrial DNA data did not resolve the relationships between D. nanus and three D. moiwanus subspecies, the nuclear DNA data indicate the monophyly of D. moiwanus and its subspecies. The nuclear gene genealogy suggests that isolated wetlands used by larval D. moiwanus derive from the ancestral riverine habitats of D. nanus and other Davidius species. The COI sequence divergence among local populations was much greater in D. moiwanus than in D. nanus, which may be the result of differences in the dispersal ranges associated with the habitat types of these species.

Comparative historical biogeography of Plateumaris leaf beetles (Coleoptera: Chrysomelidae) in Japan: interplay between fossil and molecular data

“…, 2004; Sota et al. , 2004; Kiyoshi & Sota, 2006) were based on molecular clocks from other studies (e.g. Brower, 1994; Su et al.…”

Unravelling the effects of contemporary and historical range expansion on the distribution of genetic diversity in the damselfly Coenagrion scitulum

“…We pooled all data and estimated the time since historical post‐glacial expansion using the equation τ = 2ut (Rogers & Harpending, ), where t is the time since expansion (expressed as number of generations) and u is the mutation rate per generation for the sequence under study (with u = Qk, where k is the length of the sequence and Q the divergence rate). We used two commonly used divergence rates for insect mitochondrial DNA (Brower, ; μ = 2.3% Myr‐1, Papadopoulou et al ., ;3.54% Myr‐1) and a divergence rate specifically for an Odonate species (Kiyoshi & Sota, ; 3.1 % Myr‐1). A generation time of one year was set (Cayrou & Céréghino, ).…”

Postglacial range shift and demographic expansion of the marine intertidal snail Batillaria attramentaria

“…Consequently, tremendous fluctuations in sea level and temperature accompanying connections or disconnections of lands became to draw scientific attention to their role in biogeographic changes in both terrestrial and marine taxa during the Quaternary in East Asia (Amano ; Kiyoshi and Sota ; Zhang and Sota ; Sekin et al. ).…”