Differentiation of Strains of Staphylococcus epidermidis Isolated From Bovine Udders

Abstract

SUMMARYStrains of Staphylococcus epidermidis isolated from bovine udders were differentiated by comparing biochemical tests, serological typing of proteolytic enzymes, colonial morphology, and the spectrophotometric analysis of pigments extracted with methanol. The cultures were divided into proteinasepositive and proteinase-negative groups, based on their proteolytic activity on skim milk agar, staphylococcus medium no. 110 (Difco), and gelatin. Most of the proteinase-negative cultures produced acetoin, whereas the opposite was true for the proteinase-positive cultures. A further subdivision of the cultures in each group was made by using Baird-Parker's (1963) biochemical subgrouping scheme. The proteinase-positive cultures were also subdivided by serological typing of their proteolytic enzymes into five groups, B, F, G, H, and NR, a non-reacting group. Approximately three-quarters of the cultures in the first four proteinase groups could be placed in one of Baird-Parker's biochemical subgroups. The cultures in the NR group, however, were sufficiently distinctive in their biochemical reactions to be considered as a separate biochemical subgroup. The colonies formed by the cultures were classified into five types, each consisting of two to four forms. No absolute relationship was found between the spectral absorption curves of the pigments and proteinase groups, although there were differences in the types of absorption curves that predominated in each proteinase group. Nine cultures which produced coagulase were more closely related to S. epidermidis than to S. aureus in that they did not utilize mannitol anaerobically or produce a-or ,!?-type haemolysis, they were non-pigmented and were less active biochemically. Also, serological typing of the proteolytic enzymes of three of these cultures resulted in one being classified as group G and two as group H. Group H contained only these two cultures. In contrast 27 cultures of S. aureus were classified as group A. Except for coagulase production, the biochemical reactions and the serological grouping of the proteolytic enzymes suggested a classification of S. epidermidis for the nine cultures.

Coagulase and Heat‐resistant Nuclease Producing Staphylococcus epidermidis Strains from Animals

“…Most S. epidermidis strains isolated from the skin of pigs belong to this biotype (Baird-Parker 19656;Smith & Bettge, 1972). The S. hyicus strain NCTC 10350 studied by Baird-Parker (1965~) and the bovine coagulase-positive strains found by Brown et al (1967) were classified by these authors as S. epidermidis biotype 2. The S. hyicus collection strain NCTC 10350 (ATCC 11249) appears to be somewhat atypical because it was the only strain which did not produce acid from glycerol (Table 4).…”

Sensitivity to Novobiocin and Lysozyme in the Classification of Micrococcaceae

“…Most of the strains were classified in the subgroups proposed by Baird -Parker (1965), but the 4 atypical bovine strains of Staph. aureus (Brown et al, 1967) were not classifiable in Baird-Parker's scheme. All strains of Staph.…”

The classification of staphylococci from colonized ventriculo-atrial shunts

“…The test must be read within 20 to 30 seconds of the addition of alkali, since the bright colour fades irreversibly after this. A similar medium was tested and subsequently used routinely by Brown, Sandvik, Scherer, and Rose (1967). Baird-Parker (1966) recommends testing for phosphatase production at 30°C; at his suggestion, therefore, about 100 of these strains of cocci were retested in exactly the same way excepting that they were incubated at 30°C for seven days and no difference in results was noted.…”