Differentiation of stem cells from the apical papilla into osteoblasts by the elastic modulus of porous silk fibroin scaffolds

Amirikia, Mehdi; Jorsaraei, Seyed Gholam Ali; Shariatzadeh, Mohammad Ali; Mehranjani, Malek Soleimani

doi:10.1016/j.biologicals.2018.10.005

Cited by 13 publications

(12 citation statements)

References 41 publications

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“…CCK-8 and LIVE/DEAD assays were used to confirm the proliferation ability and viability of 3D-cultured PDLSCs, which are frequently used methods in 2D culture experiments [37][38][39][40]. The CCK-8 assay revealed that the absorbance in the 2D culture increased over time while in the 3D culture it decreased over time.…”

Section: Discussionmentioning

confidence: 99%

Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study

et al. 2021

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This study compared the characteristics of periodontal ligament stem cells (PDLSCs) cultured using 3-dimensional (3D) versus conventional 2-dimensional (2D) methods. PDLSCs were cultured in either a 3D culture with a non-adhesive culture plate (Stemfit 3D®) or a conventional 2D culture using a 6-well plate. Morphology, viability, proliferation ability, and osteogenic differentiation were analyzed to characterize the differences induced in identical PDLSCs by 3D and 2D culture environments. In addition, gene expression was analyzed using RNA sequencing to further characterize the functional differences. The diameter and the viability of the 3D-cultured PDLSCs decreased over time, but the shape of the spheroid was maintained for 20 days. Although osteogenic differentiation occurred in both the 2D- and 3D-cultured PDLSCs, compared to the control group it was 20.8 and 1.6 higher in the 3D- and 2D-cultured cells, respectively. RNA sequencing revealed that PDLSCs cultured using 2D and 3D methods have different gene expression profiles. The viability of the 3D-cultured cells was decreased, but they showed superior osteogenic differentiation compared to 2D-cultured cells. Within the limitations of this study, the results demonstrate that the structure and function of PDLSCs are influenced by the cell culture method.

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Section: Discussionmentioning

confidence: 99%

Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study

et al. 2021

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“…The elastic modulus effect of RSF substrate on cell behaviors have been reported by Amirikia et al. [ 134 ]. In this study, series of RSF porous scaffolds were prepared by freeze drying through SF solution with varied concentrations.…”

Section: Advanced Biomedical Applications Of Rsf Materialsmentioning

confidence: 90%

“…In this study, series of RSF porous scaffolds were prepared by freeze drying through SF solution with varied concentrations. The produced scaffolds presented minimal changes in the pore size but with elastic modulus from 16 to 131 kPa in the wet condition [ 134 ]. Then, apical papilla stem cells were choosing to evaluate cell adhesion and osteogenesis.…”

Section: Advanced Biomedical Applications Of Rsf Materialsmentioning

confidence: 99%

Bioinspired silk fibroin materials: From silk building blocks extraction and reconstruction to advanced biomedical applications

Yao

Zou

Fan

et al. 2022

Materials Today Bio

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“…From 15 publications analyzed, CD105 was described to be expressed on SHED in 14 publications, while CD73 and CD90 were described to be expressed in 9 and 10 publications [58,[66][67][68][69][70][71][72][73][74][75][76][77][78][79]. The analysis of marker expression on SCAP was as follows: 15 publications were analyzed, and the most described markers expressed were CD146 (13), CD73 (8), CD90 (8), and CD105 (8) [27,66,[80][81][82][83][84][85][86][87][88][89][90][91][92]. SCAP is the dental stem cell type where CD146 is described as the most prominent positive marker.…”

Section: Isolation and Characterization Of Stem Cells From Dental Tissuesmentioning

confidence: 99%

Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration

Shoushrah

Transfeld

Tonk

et al. 2021

IJMS

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Dental stem cells have been isolated from the medical waste of various dental tissues. They have been characterized by numerous markers, which are evaluated herein and differentiated into multiple cell types. They can also be used to generate cell lines and iPSCs for long-term in vitro research. Methods for utilizing these stem cells including cellular systems such as organoids or cell sheets, cell-free systems such as exosomes, and scaffold-based approaches with and without drug release concepts are reported in this review and presented with new pictures for clarification. These in vitro applications can be deployed in disease modeling and subsequent pharmaceutical research and also pave the way for tissue regeneration. The main focus herein is on the potential of dental stem cells for hard tissue regeneration, especially bone, by evaluating their potential for osteogenesis and angiogenesis, and the regulation of these two processes by growth factors and environmental stimulators. Current in vitro and in vivo publications show numerous benefits of using dental stem cells for research purposes and hard tissue regeneration. However, only a few clinical trials currently exist. The goal of this review is to pinpoint this imbalance and encourage scientists to pick up this research and proceed one step further to translation.

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Differentiation of stem cells from the apical papilla into osteoblasts by the elastic modulus of porous silk fibroin scaffolds

Cited by 13 publications

References 41 publications

Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study

Comparison of 2- and 3-Dimensional Cultured Periodontal Ligament Stem Cells; a Pilot Study

Bioinspired silk fibroin materials: From silk building blocks extraction and reconstruction to advanced biomedical applications

Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration

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