Differentiation of stem cells from human deciduous and permanent teeth into spiral ganglion neuron-like cells

Gonmanee, Thanasup; Thonabulsombat, Charoensri; Vongsavan, Kutkao; Sritanaudomchai, Hathaitip

doi:10.1016/j.archoralbio.2018.01.011

Cited by 40 publications

(45 citation statements)

References 24 publications

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“…These neurotrophins are generally recognized as potent stimulators of neural differentiation. For example, the addition of GDNF or FGF to the culture medium induced a neural differentiation of MSCs (Gonmanee, Thonabulsombat, Vongsavan, & Sritanaudomchai, ; Shokohi, Nabiuni, Irian, & Miyan, ). Notwithstanding, some differential effects can be highlighted.…”

Section: Discussionmentioning

confidence: 99%

Neural differentiation of human adipose‐derived mesenchymal stem cells induced by glial cell conditioned media

Furno

Mannino

Giuffrida

et al. 2018

Journal Cellular Physiology

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Adipose-derived mesenchymal stem cells (ASCs) may transdifferentiate into cells belonging to mesodermal, endodermal, and ectodermal lineages. The aim of this study was to verify whether a neural differentiation of ASCs could be induced by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). ASCs were isolated from the stromal vascular fraction of adipose tissue and expanded for 2-3 passages. They were then cultured in OEC-CM or SC-CM for 24 hr or 7 days. At each stage, the cells were tested by immunocytochemistry and flow cytometer analysis to evaluate the expression of typical neural markers such as Nestin, PGP 9.5, MAP2, Synapsin I, and GFAP. Results show that both conditioned media induced similar positive effects, as all tested markers were overexpressed, especially at day 7. Overall, an evident trend toward neuronal or glial differentiation was not clearly detectable in many cases. Nevertheless, a higher tendency toward a neuronal phenotype was recognized for OEC-CM (considering MAP2 increases). On the other hand, SC-CM would be responsible for a more marked glial induction (considering GFAP increases). These findings confirm that environmental features can induce ASCs toward a neural differentiation, either as neuronal or glial elements. Rather than supplementing the culture medium by adding chemical agents, a "more physiological" condition was obtained here by means of soluble factors (cytokines/growth factors) likely released by glial cells. This culture strategy may provide valuable information in the development of cell-based therapeutic approaches for pathologies affecting the central/peripheral nervous system.

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Section: Discussionmentioning

confidence: 99%

Neural differentiation of human adipose‐derived mesenchymal stem cells induced by glial cell conditioned media

Furno

Mannino

Giuffrida

et al. 2018

Journal Cellular Physiology

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“…Thus, tooth regeneration using pluripotent stem cells originating from any tissue in the oral cavity is desirable [17]. The isolation of the stem cells from dental pulp was first reported by Gronthos et al [18], and stem cells originating from human deciduous teeth were also previously reported [19,20].…”

Section: Discussionmentioning

confidence: 99%

Calcified Nodule Formation <i>in Vitro</i> Using Rat Mandibular Incisor Pulp Cells

Ikenaga¹,

Yoshikawa²,

Miyamoto³

et al. 2018

JBiSE

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“…39,40 In a model of rat sciatic nerve injury, dental stem cells presented neurotrophic traits and had the ability to induce axon regeneration. 46 Moreover, it has been reported that intravenously administered DPSCs could migrate into ischaemic areas, attenuate stroke-induced inflammation and reduce infarct volumes and cerebral oedema. 39 Moreover, exosomes or DSCs-conditioned medium containing exosomes is beneficial for diseases recovering.…”

Section: Usag E Of Dental S Tem Cell S In Nervous Sys Tem Impairmentmentioning

confidence: 99%

“…The DPSCs and SHEDs differentiated cells showed comparable functional neural activities, indicating that both adult and adolescent teeth could be considered as a cell-based therapy source for neural diseases treatments. 46 Moreover, it has been reported that intravenously administered DPSCs could migrate into ischaemic areas, attenuate stroke-induced inflammation and reduce infarct volumes and cerebral oedema. 47 Based on these above evidences, we hypothesize that DSCs could be ideal stem cell sources for central nerve repair.…”

Section: Usag E Of Dental S Tem Cell S In Nervous Sys Tem Impairmentmentioning

confidence: 99%

Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

Wang

Tian

et al. 2019

Cell Proliferation

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Nerous system diseases, both central and peripheral, bring an incredible burden onto patients and enormously reduce their quality of life. Currently, there are still no effective treatments to repair nerve lesions that do not have side effects. Stem cellbased therapies, especially those using dental stem cells, bring new hope to neural diseases. Dental stem cells, derived from the neural crest, have many characteristics that are similar to neural cells, indicating that they can be an ideal source of cells for neural regeneration and repair. This review summarizes the neural traits of all the dental cell types, including DPSCs, PDLCs, DFCs, APSCs and their potential applications in nervous system diseases. We have summed up the advantages of dental stem cells in neural repair, such as their neurotrophic and neuroprotective traits, easy harvest and low rejective reaction rate, among others. Taken together, dental stem cells are an ideal cell source for neural tissue regeneration and repair.

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Differentiation of stem cells from human deciduous and permanent teeth into spiral ganglion neuron-like cells

Cited by 40 publications

References 24 publications

Neural differentiation of human adipose‐derived mesenchymal stem cells induced by glial cell conditioned media

Neural differentiation of human adipose‐derived mesenchymal stem cells induced by glial cell conditioned media

Calcified Nodule Formation <i>in Vitro</i> Using Rat Mandibular Incisor Pulp Cells

Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

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Differentiation of stem cells from human deciduous and permanent teeth into spiral ganglion neuron-like cells

Cited by 40 publications

References 24 publications

Neural differentiation of human adipose‐derived mesenchymal stem cells induced by glial cell conditioned media

Neural differentiation of human adipose‐derived mesenchymal stem cells induced by glial cell conditioned media

Calcified Nodule Formation &lt;i&gt;in Vitro&lt;/i&gt; Using Rat Mandibular Incisor Pulp Cells

Advances of tooth‐derived stem cells in neural diseases treatments and nerve tissue regeneration

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Calcified Nodule Formation <i>in Vitro</i> Using Rat Mandibular Incisor Pulp Cells