Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes

Simonović, Jelena; Toljic, Bosko; Nikolic, Nadja; Perić, Mina; Vujin, Jasna; Panajotović, Radmila; Gajić, Radoš; Bekyarova, Elena; Cataldi, Amelia; Parpura, Vladimir; Milašin, Jelena

doi:10.1002/jbm.a.36461

Cited by 35 publications

(26 citation statements)

References 41 publications

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“…. It has been demonstrated that CNTs can improve neurons responsiveness through creating tight physical interactions with cell membranes and stimulate the formation of active synaptic contacts between cells in culture …”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated that CNTs can improve neurons responsiveness through creating tight physical interactions with cell membranes and stimulate the formation of active synaptic contacts between cells in culture. [30][31][32] Fiber diameter is a very important feature of scaffolds that plays an active role in cell attachment and differentiation. It has been shown that aligned nanofibers with smaller diameters can promote neurite outgrowth compared with random nanofibers.…”

Section: Discussionmentioning

confidence: 99%

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Preparation of fibrin gel scaffolds containing MWCNT/PU nanofibers for neural tissue engineering

Hasanzadeh

Ebrahimi‐Barough

Mirzaei

et al. 2019

J Biomedical Materials Res

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Compared to the peripheral nervous system, in the central nervous system (CNS) disorders, neurons are less able to regenerate and reconstruct the neural tissue. Tissue engineering is considered as a promising approach for neural regeneration and restoring neurologic function after CNS injuries. Nanofibrous hydrogels have been recently used as three‐dimensional (3D) scaffolds for tissue engineering applications. In this kind of composites, hydrogels are incorporated with fibers to enhance their poor mechanical properties. Furthermore, introducing meshes within hydrogels can result in composites associated with advantages of both components. In the present study, we have prepared 3D nanofibrous hydrogel scaffolds based on fibrin/polyurethane/multiwall carbon nanotube (fibrin/PU/MWCNT), for application as composite scaffolds for neural tissue engineering. The fabricated fibrin/PU/MWCNT hydrogel scaffolds were characterized and their ability to support cell attachment and viability was assessed in comparison with fibrin hydrogel. Scanning electron microscopy (SEM) analysis was performed to examine the microstructural features of scaffolds. The rate of biodegradation and rheological properties of scaffolds were also investigated. After isolation of human endometrial stem cells (hEnSCs), they were cultured into the scaffolds, then their attachment and viability were assessed through SEM analysis, MTT assay and DAPI staining. Based on the results, the viability and proliferation of hEnSCs in the fibrin/PU/MWCNT hydrogels were higher than those in fibrin hydrogels. Therefore, our novel fabricated fibrin/PU/MWCNT hydrogel is able to support cell proliferation and can be used as a scaffold to provide an appropriate microenvironment for enhancing cell viability. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 802–814, 2019.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Preparation of fibrin gel scaffolds containing MWCNT/PU nanofibers for neural tissue engineering

Hasanzadeh

Ebrahimi‐Barough

Mirzaei

et al. 2019

J Biomedical Materials Res

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“…Such a nano-platform can be potentially utilized for the promoting neurite outgrowth and cell differentiation in the close perspective. In another work, the single-walled CNTs have been used to enhance neural differentiation of stem cells from apical papilla [148].…”

Section: Carbon Nanotubes and Stem Cellsmentioning

confidence: 99%

The Advances in Biomedical Applications of Carbon Nanotubes

Saliev

2019

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Unique chemical, physical, and biological features of carbon nanotubes make them an ideal candidate for myriad applications in industry and biomedicine. Carbon nanotubes have excellent electrical and thermal conductivity, high biocompatibility, flexibility, resistance to corrosion, nano-size, and a high surface area, which can be tailored and functionalized on demand. This review discusses the progress and main fields of bio-medical applications of carbon nanotubes based on recently-published reports. It encompasses the synthesis of carbon nanotubes and their application for bio-sensing, cancer treatment, hyperthermia induction, antibacterial therapy, and tissue engineering. Other areas of carbon nanotube applications were out of the scope of this review. Special attention has been paid to the problem of the toxicity of carbon nanotubes.

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“…Recent studies have demonstrated the characteristics and capacity for multilineage differentiation of hSCAPs (26). Among the various dental-origin stem cell populations, hSCAPs exhibited superior pro ling, including multipotential differentiation, secretion of neurotrophic factors, and neurite outgrowth stimulation (27).…”

Section: Discussionmentioning

confidence: 99%

Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

Songsaad

Gonmanee

Ruangsawasdi

et al. 2020

Preprint

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Introduction: Stem cell transplantation of exogenous neural progenitor cells (NPCs) derived from mesenchymal stem cells (MSCs) has emerged as a promising approach of neurodegenerative disease. Human stem cells from apical papilla (hSCAPs) are derived from migratory neural crest stem cells, and exhibit a potential of neuronal differentiation. However, their neuronal differentiation is low and unpredictable. Resveratrol has been described as a sirtuin 1 (SIRT1) activator which plays an important role in enhancing neuronal differentiation. In this study, we investigate the potential of resveratrol as an enhancer on neuronal differentiation through NPCs induction of hSCAPs.Methods: Stem cells were isolated from human apical papilla, and characterized as MSCs. The cellular toxicity of resveratrol treatment to the characterized hSCAPs was investigated by MTT assay. The non-cellular toxicity concentrations of resveratrol were assessed with various pre-treatment times to select the optimal condition that highly expressed the neural progenitor gene, NES. Consequently, the optimal condition of resveratrol pre-treatment was synergistically performed with a neuronal induction medium to trigger neuronal differentiation. The differentiated cells were visualized, the genes profiling was quantified, and the percentage of neuronal differentiation was calculated. Moreover, the intracellular calcium oscillation was demonstrated. Results: The cellular toxicity of resveratrol was not observed for up to 50 µM for 12 hours. Interestingly, hSCAPs pre-treated with 10 µM resveratrol for 12 hours (RSV-hSCAPs) significantly expressed NES, which is determined as the optimal condition. Under neuronal induction, both of hSCAPs and RSV-hSCAPs were differentiated (d-hSCAPs, and RSV-d-hSCAPs) as they exhibited neuronal-like appearances with Nissl substance staining. The highest expression of NES, and SOX1 was observed in RSV-d-hSCAPs. Additionally, the percentage of neuronal differentiation of RSV-d-hSCAPs was significantly higher than d-hSCAPs for 4 times. Importantly, the neuronal-like cells exhibited slightly increasing pattern of calcium intensity.Conclusion: This study demonstrated that pre-treatment of resveratrol strongly induces neural progenitor marker gene expression which synergistically enhances neural progenitor-like cells’ induction with neuronal induction medium.

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Differentiation of stem cells from apical papilla into neural lineage using graphene dispersion and single walled carbon nanotubes

Cited by 35 publications

References 41 publications

Preparation of fibrin gel scaffolds containing MWCNT/PU nanofibers for neural tissue engineering

Preparation of fibrin gel scaffolds containing MWCNT/PU nanofibers for neural tissue engineering

The Advances in Biomedical Applications of Carbon Nanotubes

Potential of resveratrol in enrichment of neural progenitor-like cells induction of human stem cells from apical papilla

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