Differentiation of porcine dendritic cells by granulocyte-macrophage colony-stimulating factor expressed in Pichia pastoris

Foss, Dennis L.; Bennaars, Antoinette M.; Pennell, Christopher A.; Moody, Max D.; Murtaugh, Michael P.

doi:10.1016/s0165-2427(03)00002-3

Cited by 12 publications

(4 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the contrary, other semen cytokines, also present in high concentration in porcine SP 11 , have barely claimed scientific interest, for instance, the granulocyte and macrophage colony stimulating factor (GM-CSF). The GM-CSF is a pro-inflammatory cytokine with a molecular weight ranging from 50 to 15 kDa according to its glycosylation degree 17 , 18 . Immune regulatory functions as well as an active involvement on growth, proliferation, maturation, and function of hematopoietic cells have been described among the roles of this cytokine 19 – 21 .…”

Section: Introductionmentioning

confidence: 99%

Granulocyte-macrophage colony stimulating factor (GM-CSF) is fully expressed in the genital tract, seminal plasma and spermatozoa of male pigs

Padilla

Martínez-Hernández

Barranco

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a pro-inflammatory cytokine identified in boar seminal plasma (SP) but until now unexplored in terms of place of production and its association to spermatozoa. This study aimed to explore these aspects by evaluating the presence of GM-CSF in porcine reproductive organs (testes, epididymis and accessory sex glands), SP and mature spermatozoa (from cauda epididymis and ejaculated) using Western blot (WB), immunohistochemistry and immunocytochemistry. Positive labelling was obtained in tissues, SP and spermatozoa. In reproductive organs, WB revealed three forms of GM-CSF with different glycosylation degrees (15, 31 and 40 kDa). In SP and epididymal fluid, the GM-CSF appeared only in its active form while in spermatozoa the GM-CSF form present varied among sperm sources. Non-viable spermatozoa showed more GM-CSF than viable spermatozoa (14.87 ± 1.98 RU vs. 7.25 ± 0.52 RU) of fluorescence intensity. In conclusion, GM-CSF is widely present in the reproductive tract of male pigs, attached to the spermatozoa already in the epididymis as well as verted to SP. Consequently, the GM-CSF ought to regulate male genital tract and sperm function as well as mediating initial inflammatory responses and further mediating later immune actions by the female to semen deposition.

show abstract

Section: Introductionmentioning

confidence: 99%

Granulocyte-macrophage colony stimulating factor (GM-CSF) is fully expressed in the genital tract, seminal plasma and spermatozoa of male pigs

Padilla

Martínez-Hernández

Barranco

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…However, each isozyme possesses diVerent substrate speciWcity and thermal stability for biocatalysis, which might lead to divergent catalytic performances [5]. Cloning and expression of the major lipase gene (lip1) is the most acceptable approach for obtaining pure C. rugosa lipase (CRL) with optimized properties for commercial use [3,6,7].…”

Section: Introductionmentioning

confidence: 99%

Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter

Zhao¹,

Wang²,

Deng³

et al. 2007

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

The high-cell-density fermentation of Candida rugosa lipase in the constitutive Pichia pastoris expression system was scaled up from 5 to 800 l in series by optimizing the fermentation conditions at both lab scale and pilot scale. The exponential feeding combined with pH-stat strategy succeeded in small scale studies, while a two-stage fermentation strategy, which shifted at 48 h by fine tuning the culture temperature and pH, was assessed effective in pilot-scale fermentation. The two-stage strategy made an excellent balance between the expression of heterogeneous protein and the growth of host cells, controlling the fermentation at a relatively low cell growth rate for the constitutive yeast expression system to accumulate high-level product. A stable lipase activity of approximately 14,000 IU ml(-1) and a cell wet weight of ca. 500 g l(-1) at the 800-l scale were obtained. The efficient and convenient techniques suggested in this study might facilitate further scale-up for industrial lipase production.

show abstract

“…Bautista et al [28] have also demonstrated that IL-4 can be replaced by IL-13 for the generation of MoDC. Phenotypically, porcine MoDC are characterized as CD1 + CD14 + CD16 + CD80/86 + CD172a + and MHC class II + [23,24,[28][29][30] (Table 1). From a comparative immunological point of view, it was unexpected to find CD14, because human CD14 is considered to be a typical monocyte/macrophage rather than a DC marker [8].…”

mentioning

confidence: 99%

The porcine dendritic cell family

Summerfield¹,

McCullough²

2009

Developmental & Comparative Immunology

120

View full text Add to dashboard Cite

Considering the pivotal roles played by dendritic cells (DCs) in both innate and adaptive immune responses, advances in the field of porcine immunology DC biology have recently progressed rapidly. As with the more extensively studied murine and human DCs, porcine DC can be generated from bone marrow haematopoietic cells or monocytes, and have been analysed in various immunological and non-immunological tissues. Both conventional DC (cDC) and plasmacytoid DC (pDC) have been characterized. The function of porcine monocyte-derived DC has not only been characterized in terms of antigen presentation and lymphocyte activation, but also their response to various ligands of pattern recognition receptors. These have been characterized in terms of the induction of DC maturation and pro-inflammatory, Th1-like or Th2-like cytokines secretion. Porcine pDC most effectively sense virus infections and are characterized by their capacity to produce large quantities of IFN-alpha and the pro-inflammatory cytokines TNF-alpha, IL-6 and IL-12. As such, the DC family as a whole is a powerful ally in the host battle against pathogen attack. Nevertheless, DC in particular tissue environments or under particular stimuli can down-regulate immune response development. This is not only important for preventing over-activation of the immune system and also for ensuring tolerance against self or "friendly" substances including food components, but may also be used as a mechanism of pathogens to evade immune responses.

show abstract

Differentiation of porcine dendritic cells by granulocyte-macrophage colony-stimulating factor expressed in Pichia pastoris

Cited by 12 publications

References 46 publications

Granulocyte-macrophage colony stimulating factor (GM-CSF) is fully expressed in the genital tract, seminal plasma and spermatozoa of male pigs

Granulocyte-macrophage colony stimulating factor (GM-CSF) is fully expressed in the genital tract, seminal plasma and spermatozoa of male pigs

Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter

The porcine dendritic cell family

Contact Info

Product

Resources

About