Differentiation of PC12 cell line into neuron by Valproic acid encapsulated in the stabilized core-shell liposome-chitosan Nano carriers

Kelkawi, Ali Hamad Abd; Hashemzadeh, Hadi; Pashandi, Zaiddodine; Tiraihi, Taki; Naderi‐Manesh, Hossein

doi:10.1016/j.ijbiomac.2022.05.021

Cited by 7 publications

(5 citation statements)

References 55 publications

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“…TEM (Figure h) revealed that OLA-lips exhibited a uniform and spherical morphology. The ζ-potential of OLA-lips was measured to be −27.5 ± 1.4 mV (Figure S1), indicating a stable liposome structure due to nanoparticle repulsion . The particle size of OLA-lips remained constant after 72 h (Figure i), suggesting its good stability.…”

Section: Resultsmentioning

confidence: 94%

“…The ζ-potential of OLA-lips was measured to be −27.5 ± 1.4 mV (Figure S1), indicating a stable liposome structure due to nanoparticle repulsion. 33 The particle size of OLA-lips remained constant after 72 h (Figure 1i), suggesting its good stability. Additionally, the drug loading rate and encapsulation efficiency of OLA in OLA-lips were (9.58 ± 0.84)% and (53.00 ± 5.14)%, respectively, indicating that the hydrophobic drug OLA was successfully loaded into the phospholipid bilayer of OLA-lips.…”

Section: Preparation and Characterization Of Ola-lipsmentioning

confidence: 91%

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Chitosan Thermosensitive Hydrogel Based on DNA Damage Repair Inhibition and Mild Photothermal Therapy for Enhanced Antitumor Treatment

et al. 2023

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The DNA damage repair of tumor cells limits the effect of photothermal therapy (PTT), and high temperatures induced by PTT can damage adjacent normal tissues. To overcome these limitations, we developed a novel composite hydrogel (OLA-Au-Gel) based on chitosan (CS) and β-glycerophosphate (β-GP), which encapsulated olaparib-liposomes (OLA-lips) and CS-capped gold nanoparticles (CS-AuNPs). OLA-Au-Gel achieved the combination of mild PTT (mPTT) by CS-AuNPs and tumor DNA damage repair inhibition by OLA. The hydrogel showed good biocompatibility, injectability, and photothermal response. Under near-infrared laser irradiation, OLA-Au-Gel inhibited the proliferation of tumor cells, induced the generation of reactive oxygen species in vitro, and effectively inhibited the growth of breast tumors in vivo. OLA-Au-Gel shows a promising application prospect for inhibiting tumor development and improving the antitumor effect. Collectively, we propose a novel strategy for enhanced antitumor therapy based on the combination of mPTT and DNA damage repair inhibition.

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Section: Resultsmentioning

confidence: 94%

Section: Preparation and Characterization Of Ola-lipsmentioning

confidence: 91%

Chitosan Thermosensitive Hydrogel Based on DNA Damage Repair Inhibition and Mild Photothermal Therapy for Enhanced Antitumor Treatment

et al. 2023

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“…For example, it could stimulate neurite outgrowth and prolong cell survival via the ERK [ 45 ] and JNK [ 1 ] pathway. In addition, VPA could induce the differentiation of PC12 cells which are the neuroblastoma cell into mature neurons [ 48 ]. Other groups were reported that adipose tissue-derived stem cells could also differentiate into neuronal cells by treating VPA [ 12 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of HDAC inhibitors on neuroblastoma SH-SY5Y cell differentiation into mature neurons via the Wnt signaling pathway

et al. 2023

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Histone deacetylase (HDAC) inhibitors affect cell homeostasis, gene expression, and cell cycle progression and promote cell terminal differentiation or apoptosis. However, the effect of HDAC inhibition on SH-SY5Y cells, which are neuroblastoma cells capable of differentiating into neurons under specific conditions, such as in the presence of retinoic acid (RA), is unknown. In this study, we hypothesized that HDAC inhibitors induced the neuronal differentiation of SH-SY5Y cells. To test this hypothesis, we used phase contrast microscopy, immunocytochemistry (ICC), qPCR, and western blotting analysis. MS-275 and valproic acid (VPA), two HDAC inhibitors, were selected to evaluate neuronal differentiation. It was confirmed that cells treated with MS-275 or VPA differentiated into mature neurons, which were distinguished by bipolar or multipolar morphologies with elongated branches. In addition, the mRNA expression of neuronal markers (Tuj1 and NEFH) and the oligodendrocyte marker (CNP) was significantly increased with MS-275 or VPA treatment compared to that with RA treatment. In addition, the protein expression of the other neuronal markers, Tuj1 and NeuN, was highly increased with HDAC inhibitor treatments compared to that with RA treatment. Furthermore, we confirmed that noncanonical Wnt signaling was upregulated by HDAC inhibitors via MAPK signaling and the Wnt/JNK pathway. Therefore, both MS-275 and VPA promoted the differentiation of SH-SY5Y cells into mature neurons via the Wnt signaling pathway.

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“…The TP entrapped into zein and formed water-insoluble particles were probably responsible for the lower retention rate [18]. The crosslinking improved the TP retention rate slightly compared to films without crosslinking [22]. TG crosslinking Polymers 2023, 15, 2882 6 of 16 (TG1TP1, TG2TP1, TG1TP2, and TG2TP2) increased the retention rate to 36-39%.…”

Section: Retentivity and Release Of Tp From The Filmmentioning

confidence: 99%

Encapsulation of Tea Polyphenol in Zein through Complex Coacervation Technique to Control the Release of the Phenolic Compound from Gelatin–Zein Composite Film

Ahammed,

Easdani,

Liu

et al. 2023

Polymers

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Green tea polyphenol (TP) was encapsulated in zein and fabricated into a gelatin–zein composite film by complex coacervation. Transglutaminase (TG) crosslinking was employed to obtain a compact structural orientation of the film to prolong the release of bioactive compounds. The encapsulation efficiency of zein and the TP release rate from the composite film were investigated. The retention rate was over 30% and 80% after film fabrication and storage, respectively. Crosslinking decreased the diffusion coefficient by half, thus improving the release of TP from the film. The antioxidant properties were satisfactory after discharge from the film detected by DPPH/ABTS scavenging. The value of crosslinking degree (~60%) and increased molecular weight of the protein were investigated by SDS-PAGE, indicating the compatibility of TP and TG treatment. According to physicomechanical findings, the TG2TP1 film exhibited the best characteristics. Tensile strength and water solubility properties were ameliorated by the TG treatment of TP-encapsulated films compared to the control film. TG and TP-loaded gelatin–zein composite film had better thermal stability than the control film. Moreover, the TP loading reduced the transparency value and improved the light-barrier properties of the film. The films showed significant antimicrobial activities against two food-borne bacteria, including Staphylococcus aureus BCTC13962 and Escherichia coli BCRC10675. The result obtained shows that the encapsulation of TP and TG treatment may be used to fabricate gelatin–zein composite film with controlled release of phenolic compounds for active packaging applications.

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Differentiation of PC12 cell line into neuron by Valproic acid encapsulated in the stabilized core-shell liposome-chitosan Nano carriers

Cited by 7 publications

References 55 publications

Chitosan Thermosensitive Hydrogel Based on DNA Damage Repair Inhibition and Mild Photothermal Therapy for Enhanced Antitumor Treatment

Chitosan Thermosensitive Hydrogel Based on DNA Damage Repair Inhibition and Mild Photothermal Therapy for Enhanced Antitumor Treatment

Effects of HDAC inhibitors on neuroblastoma SH-SY5Y cell differentiation into mature neurons via the Wnt signaling pathway

Encapsulation of Tea Polyphenol in Zein through Complex Coacervation Technique to Control the Release of the Phenolic Compound from Gelatin–Zein Composite Film

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