Differentiation of osteoblasts and formation of mineralized bone in vitro

Tenenbaum, Howard C.; Heersche, J. N. M.

doi:10.1007/bf02411212

Cited by 203 publications

(110 citation statements)

References 19 publications

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“…This effect was observed only when resveratrol was administered during the late stages of osteogenesis (i.e. days 4-6 in the CPO model), pointing toward the presence of a resveratrol-responsive cell population that is more fully differentiated (Tenenbaum 1981, Tenenbaum & Heersche 1982. This phenomenon has recently been observed and reported elsewhere.…”

Section: Differentiationsupporting

confidence: 63%

“…The CPO model has been described in detail previously (Nijweide 1975, Tenenbaum & Heersche 1982) and has been shown to produce morphotypical bone reliably within a 6-day culture period. Of course, cell culture systems also have unique advantages that may be exploited as outlined below ).…”

Section: Rationale For Use Of Proposed In Vitro Modelsmentioning

confidence: 99%

“…CPO culture Folded periosteal explants from 17-dayold embryonic chicks were cultured as described previously in detail (Nijweide 1975, Tenenbaum & Heersche 1982. Media changes occurred on days 2 and 4, and the medium comprised BGJ b medium (Fitton-Jackson modification) with -glutamine (Gibco, Grand Island, NY, USA) and supplemented with 2% antibiotic (10 000 units/ml penicillin G sodium, 10 000 µg/ml streptomycin sulfate in 0·85% saline) (Gibco), 10% fetal calf serum (Gibco), 10 7 M dexamethasone (Sigma, St Louis, MO, USA) (Tenenbaum & Heersche 1985, McCulloch & Tenenbaum 1986), 10 mM -glycerophosphate (Tenenbaum et al 1989), and 300 µg/ml -ascorbate (Gibco) (Tenenbaum & Heersche 1986).…”

Section: Culture Systemsmentioning

confidence: 99%

See 2 more Smart Citations

Inhibition of dioxin effects on bone formation in vitro by a newly described aryl hydrocarbon receptor antagonist, resveratrol

Singh¹,

Casper²,

Fritz³

et al. 2000

Journal of Endocrinology

129

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Aryl hydrocarbon receptor (AhR) ligands are environmental contaminants found in cigarette smoke and other sources of air pollution. The prototypical compound is TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin), also known as dioxin. There is an increasing body of knowledge linking cigarette smoking to osteoporosis and periodontal disease, but the direct effects of smokeassociated aryl hydrocarbons on bone are not well understood. Through the use of resveratrol (3,5,4 -trihydroxystilbene), a plant antifungal compound that we have recently demonstrated to be a pure AhR antagonist, we have investigated the effects of TCDD on osteogenesis. It was postulated that TCDD would inhibit osteogenesis in bone-forming cultures and that this inhibition would be antagonized by resveratrol. We employed the chicken periosteal osteogenesis (CPO) model, which has been shown to form bone in vitro in a pattern morphologically and biochemically similar to that seen in vivo, as well as a rat stromal cell bone nodule formation model. In the CPO model, alkaline phosphatase (AP) activity was reduced by up to 50% (P<0·01 vs control) in the presence of 10 9 M TCDD and these effects were reversed by 10 6 M resveratrol (P<0·05 vs TCDD alone). TCDD-mediated inhibition of osteogenesis was restricted primarily to the osteoblastic differentiation phase (days 0-2) as later addition did not appear to have any effects. Message levels for important bone-associated proteins (in the CPO model) such as collagen type I, osteopontin, bone sialoprotein and AP were inhibited by TCDD, an effect that was antagonized by resveratrol. Similar findings were obtained using the rat stromal bone cell line. TCDD (at concentrations as low as 10 10 M) caused an approximately 33% reduction in AP activity, which was abrogated by 3·5 10 7 M resveratrol. TCDD also induced a marked reduction in mineralization (75%) which was completely antagonized by resveratrol. These data suggest that AhR ligands inhibit osteogenesis probably through inhibition of osteodifferentiation and that this effect can be antagonized by resveratrol. Since high levels of AhR ligands are found in cigarette smoke, and further since smoking is an important risk factor in both osteoporosis and periodontal disease, it may be postulated that AhR ligands are the component of cigarette smoke linking smoking to osteoporosis and periodontal disease. If so, resveratrol could prove to be a promising preventive or therapeutic agent for smoking-related bone loss.

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Section: Differentiationsupporting

confidence: 63%

Section: Rationale For Use Of Proposed In Vitro Modelsmentioning

confidence: 99%

Section: Culture Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of dioxin effects on bone formation in vitro by a newly described aryl hydrocarbon receptor antagonist, resveratrol

Singh¹,

Casper²,

Fritz³

et al. 2000

Journal of Endocrinology

129

View full text Add to dashboard Cite

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“…Recently, amorphous calcium phosphate has been stabilized by pyrophosphate ions (P 2 0 7 -4 ) and hybridized with zirconium (Zr-ACP) to retard its dissolution and subsequent conversion to HAP in aqueous environments [50,51]. In addition, Zr-ACP allows for controlled release of calcium and phosphate ions; factors that have been postulated to increase osteoblast differentiation and mineralization in vitro [52][53][54][55].…”

Section: Ceramicsmentioning

confidence: 99%

Osteoblast response to zirconia‐hybridized pyrophosphate‐stabilized amorphous calcium phosphate

Whited

Škrtić

Love

et al. 2005

J Biomedical Materials Res

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(Abstract)Biodegradable polyesters, such as poly(DL-lactic-co-glycolic acid) (PLGA), have been used to fabricate porous bone scaffolds to support bone tissue development. These scaffolds allow for cell seeding, attachment, growth and extracellular matrix production in vitro and are replaced by new bone tissue when implanted into bone sites in vivo.Hydroxyapatite (HAP) and β-tricalcium phosphate (β-TCP) ceramics have been incorporated into PLGA bone scaffolds and have been shown to increase their osteoconductivity (support cell attachment). Although HAP, β-TCP, and biodegradable polyesters are osteoconductive, there is no evidence that these scaffold materials are osteoinductive (support cell differentiation). Calcium and phosphate ions, in contrast, have been postulated to be osteogenic factors that enhance osteoblast differentiation and mineralization. Recently, a zirconia-hybridized pyrophosphate stabilized amorphous calcium phosphate (Zr-ACP) has been synthesized which permits controlled release of calcium and phosphate ions and thus is hypothesized to be osteoinductive. Incorporation of Zr-ACP into a highly porous poly(DL lactic-co-glycolic acid) (PLGA) scaffold could potentially increase the osteoinductivity of the scaffold and therefore promote osteogenesis when implanted in vivo.To determine the osteoinductivity of Zr-ACP, a MC3T3-E1 mouse calvarialderived osteoprogenitor cell line was used to measure cell response to Zr-ACP. To accomplish this objective, Zr-ACP was added to cell culture at different stages in cell maturation (days 0, 4 and 11). DNA synthesis, alkaline phosphatase (ALP) activity, iii osteopontin synthesis and collagen synthesis were determined. Results indicate that culture in the presence of Zr-ACP significantly increased cell proliferation, ALP activity and osteopontin synthesis but not collagen synthesis. To determine the feasibility of incorporating Zr-ACP into a PLGA scaffold, PLGA/Zr-ACP composite foams (5% or 10% (w/v) polymer:solvent with 25 wt% or 50 wt% Zr-ACP) were fabricated using a thermal phase inversion technique. Scanning electron microscopy revealed a highly porous structure with pores ranging in size from a few microns to about 100 µm. The amorphous structure of the Zr-ACP was maintained during composite fabrication as confirmed by X-ray diffraction measurements. Composite scaffolds also showed significantly greater compressive yield strengths and moduli as compared to pure polymer scaffolds.The results of this study indicate that Zr-ACP enhances the osteoblastic phenotype of MC3T3-E1 cells in vitro and can be incorporated into a porous PLGA scaffold. Porous PLGA/Zr-ACP composites are promising for use as bone scaffolds to heal bone defects.iv

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“…18 b-Glycerophosphate is an inorganic phosphate needed to produce hydroxyapatite mineral and has been shown in many studies to play an important role in the osteogenic differentiation of MSCs. 12,[23][24][25] It also regulates expression of genes including osteopontin and BMP-2. [26][27][28] Exposure of rat MSCs, 12,[14][15][16][17] human MSCs (hMSCs), 9,11,13 or murine osteoblasts 22,29 to dexamethasone, ascorbic acid, and b-glycerophosphate can significantly increase alkaline phosphatase (ALP) activity in vitro.…”

mentioning

confidence: 99%

Osteogenic Differentiation of Mesenchymal Stem Cells by Mimicking the Cellular Niche of the Endochondral Template

Freeman

Stevens

Owens

et al. 2016

Tissue Engineering Part A

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In vitro bone regeneration strategies that prime mesenchymal stem cells (MSCs) with chondrogenic factors, to mimic aspects of the endochondral ossification process, have been shown to promote mineralization and vascularization by MSCs both in vitro and when implanted in vivo. However, these approaches required the use of osteogenic supplements, namely dexamethasone, ascorbic acid, and b-glycerophosphate, none of which are endogenous mediators of bone formation in vivo. Rather MSCs, endothelial progenitor cells, and chondrocytes all reside in proximity within the cartilage template and might paracrineally regulate osteogenic differentiation. Thus, this study tests the hypothesis that an in vitro bone regeneration approach that mimics the cellular niche existing during endochondral ossification, through coculture of MSCs, endothelial cells, and chondrocytes, will obviate the need for extraneous osteogenic supplements and provide an alternative strategy to elicit osteogenic differentiation of MSCs and mineral production. The specific objectives of this study were to (1) mimic the cellular niche existing during endochondral ossification and (2) investigate whether osteogenic differentiation could be induced without the use of any external growth factors. To test the hypothesis, we evaluated the mineralization and vessel formation potential of (a) a novel methodology involving both chondrogenic priming and the coculture of human umbilical vein endothelial cells (HUVECs) and MSCs compared with (b) chondrogenic priming of MSCs alone, (c) addition of HUVECs to chondrogenically primed MSC aggregates, (d-f) the same experimental groups cultured in the presence of osteogenic supplements and (g) a noncoculture group cultured in the presence of osteogenic growth factors alone. Biochemical (DNA, alkaline phosphatase [ALP], calcium, CD31 + , vascular endothelial growth factor [VEGF]), histological (alcian blue, alizarin red), and immunohistological (CD31 + ) analyses were conducted to investigate osteogenic differentiation and vascularization at various time points (1, 2, and 3 weeks). The coculture methodology enhanced both osteogenesis and vasculogenesis compared with osteogenic differentiation alone, whereas osteogenic supplements inhibited the osteogenesis and vascularization (ALP, calcium, and VEGF) induced through coculture alone. Taken together, these results suggest that chondrogenic and vascular priming can obviate the need for osteogenic supplements to induce osteogenesis of human MSCs in vitro, while allowing for the formation of rudimentary vessels.

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Differentiation of osteoblasts and formation of mineralized bone in vitro

Cited by 203 publications

References 19 publications

Inhibition of dioxin effects on bone formation in vitro by a newly described aryl hydrocarbon receptor antagonist, resveratrol

Inhibition of dioxin effects on bone formation in vitro by a newly described aryl hydrocarbon receptor antagonist, resveratrol

Osteoblast response to zirconia‐hybridized pyrophosphate‐stabilized amorphous calcium phosphate

Osteogenic Differentiation of Mesenchymal Stem Cells by Mimicking the Cellular Niche of the Endochondral Template

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