Differentiation of Osteoblast and Osteoclast Cells on Hydrogenated-Tetrahedral Amorphous Carbon Coated Titanium

Abstract

To examine the effects of hydrogenated-tetrahedral amorphous carbon (ta-C:H) on differentiation of osteoblasts and osteoclasts, osteoblastic MC3T3-E1 cells and osteoclastic RAW264.7 cells were cultured on these titanium (Ti) disks with various surface roughness. Real-time quantitative reverse transcriptase-polymerase chain reaction analysis showed that the mRNA expressions of Runx2 and ALP, used as osteoblast differentiation markers, were significantly higher in MC3T3-E1 cells cultured on ta-C:H-coated Ti disks (ta-C:H Ti). On the other side, the mRNA expressions of TRAP and cathepsin K, which are osteoclast differentiation markers, were significantly lower in RANKL-treated RAW264.7 cells. These results, taken together, suggested the possibility that ta-C:H Ti simultaneously altered osteoblast and osteoclast differentiation, which could be preferable for osseointegration acquisition and long-term success of implant therapy.

Antibacterial Fluorinated Diamond-like Carbon Coating Promotes Osteogenesis—Comparison with Titanium Alloy

“…Specimens in Group C had not yet reached uncoupling. Lamellar bone, which is formed only in the remodeling phase of bone union, was generated in greater amounts in Group F than in Group C. Immature cells such as fibroblasts and fibrous osteoids were observed in Group C but not in Group F, suggesting that the remodeling phase started earlier in Group F. These findings, which suggest that bone union was more accelerated with F-DLC coated implants than with non-coated titanium alloy implants, are supported by the data showing that bone formation rate/tissue volume increase was more significant in Group F than in Group C. DLC coating without fluorine has been reported to promote bone union [22][23][24], and the bone union-promoting action of F-DLC in the present study may have been affected by the DLC in the coating as well as by the fluorine portion. Kawaguchi et al have speculated that bone union could be accelerated because the DLC coating is more hydrophilic than titanium coating [26], but that reasoning is inconsistent with the highly hydrophobic properties of the F-DLC coating.…”

“…Effects on osteogenesis are also a concern. The osteogenesis-inducing effects of DLC coating [22][23][24] are attributable in part to its highly hydrophilic properties. No studies have yet been performed on the effects of the F-DLC coating, which is highly hydrophobic, on either osteogenesis or bone resorption.…”

Investigation of Anti-Corrosive Performance of a Si-Doped DLC-Coated Magnesium Alloy Stent Deposited by RF-Plasma CVD

“…In addition, DLC can be deposited into a film for coating a material at a low temperature to reduce the thermal effect on that particular material. Currently, research on the applications of DLC is progressing in various fields, such as dental domains [19,20] and endovascular therapeutical instruments, because of the aforementioned mechanical properties, good blood compatibility, and low-temperature film deposition. The antithrombotic properties and prevention of the restenosis of DLC-coated stents have been confirmed in animal experiments (in vivo) and clinical trials of a human, suggesting their biocompatibility and safety [21][22][23][24].…”

Novel DLC Coating Technique on an Inner-wall of Extended Polytetrafluoroethylene Vascular Grafts Using Methane Plasma Produced by AC HV Discharge

“…Generally, DLC films have been attracting increasing attention to be used in industrial applications as protective films for car components and machine parts as well as in bio-compatibilized films that can be used in case of medical equipment because of their high degree of hardness, low friction coefficient, abrasion and corrosion resistance, chemical stability, biocompatibility, and gas barrier property [3][4][5]. Recently, following the production of coronary artery stents [6,7], highly biocompatible DLC films are considered for usage in medical implants such as dental implants [8,9].…”