Differentiation of normal and giant Vicia faba populations of the stem nematode Ditylenchus dipsaci: agreement between RAPD and phenotypic characteristics

Abstract

Two main race groups of the stem nematode, Ditylenchus dipsaci, attack Vicia faba. The aim of this work was to evaluate random amplified polymorphic DNA (RAPD) as a source of markers for race identification and variability analysis of D. dipsaci. Thirteen populations of D. dipsaci were tested and one population of both D. myceliophagus and D. destructor as outgroup species. Sufficient levels of variation were detected to allow a clear distinction between the two D. dipsaci groups. This result was in agreement with the characterization based on morphological differences among races and on the type of faba bean disease symptoms. The intraspecific variation within D. dipsaci populations associated with V. faba was low and no strong correlation could be found between the geographical origin and molecular or morphological characterization. RAPDs are a powerful tool for identification of D. dipsaci races and/or populations.

A fast and sensitive method for the simultaneous identification of three important nematode species of the genusDitylenchus

“…This nematode, formerly considered to be a ‘giant race’ of D. dipsaci ( Ditylenchus sp. B), is now considered to be a species distinct from D. dipsaci on the basis of body size, chromosome number, host‐plant range, biology, RLFP, ALFP and RAPD profiles and rDNA sequence . It has been reported as more damaging to its host than D. dipsaci because of more severe symptoms and more infested seeds .…”

Conversion of sequence-characterized amplified region (SCAR) bands into high-throughput DNA markers based on RAPD technique for detection of the stem nematode Ditylenchus dipsaci in crucial plant hosts

“…Therefore, an alternative molecular detection technique is needed to be developed for a reliable molecular detection and determination of the stem nematode D. dipsaci in biological materials, such as host plant tissues, soil samples etc. Alternative DNA markers for a differentiation of giant and normal types of D. dipsaci were previously characterized by SCAR and AFLP experimental approaches (Esquibet et al 1998, Esquibet et al 2003. However, these authors did not verify the developed SCAR and AFLP markers for routine molecular diagnostics purposes.…”

“…During the last several years DNA-diagnostics or population study methods including RFLP (Curran et al 1985), DNA hybridization (Burrows and Perry 1988), RAPD (Folkertsma et al 1994, Williamson et al 1997, Esquibet et al 1998and Zhang et al 1998, allele specific-PCR (Zouhar et al 2000), SCAR (Zijlstra 2000) and RAPD (Samal et al 2003, Sedlak et al 2004, have been developed for characterization and identification of plantparasitic nematodes. Specific PCR assays based on ribosomal RNA gene cluster for detection of D. dipsaci were developed (Marek et al 2005, Subbotin et al 2005.…”

Effects of heavy grazing pressure on the random amplified polymorphic DNA marker diversity of mountain rough fescue (Festuca campestris Rydb.) in south western Alberta

“…Random amplified polymorphic DNA markers have been shown to segregate in a biparental dominant Mendelian manner (Carlson et al 1991;Roy et al 1992;Heun and Helentjaris 1993). Their use as markers for the genetic characterization of populations has been well established for a variety of organisms (Chalmers et al 1992;Huff et al 1993;Caccone et al 1997;Esquibet et al 1998;Hogbin et al 1998;Lou et al 1998;Suazo et al 1998). Using RAPD markers in this study, we compared the genetic diversity of two mountain rough fescue subpopulations that had been either heavily grazed or ungrazed for 52 yr to determine whether grazing pressure affected their genetic composition.…”