Differentiation of neurosphere-derived rat neural stem cells into oligodendrocyte-like cells by repressing PDGF-α and Olig2 with triiodothyronine

Abbaszadeh, Hojjat-Allah; Tiraihi, Taki; Delshad, Alireza Azizzadeh; Saghedizadeh, Majid; Taheri, Taher; Kazemi, Hadi; Hassoun, Hayder K.

doi:10.1016/j.tice.2014.08.003

Cited by 21 publications

(29 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rats were anesthetized using intraperitoneal injection of ketamine and xylazine (80 and 10 mg/kg, respectively; both from Sigma-Aldrich). To expose rat spinal cord, the dorsal laminectomy was performed at the T12-L1 level [15,16] . The contusion model of traumatic spinal cord was induced by dropping a 10-g metal rod with 2 mm diameter from 25-mm height.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Decrease in Cavity Size and Oligodendrocyte Cell Death Using Neurosphere-Derived Oligodendrocyte-Like Cells in Spinal Cord Contusion Model

Abbaszadeh

Tiraihi

Sadeghi

et al. 2018

IBJ

Self Cite

View full text Add to dashboard Cite

Background: Oligodendrocyte cell death is among the important features of spinal cord injury, which appears within 15 min and occurs intensely for 4 h after injury, in the rat spinal contusion model. Accordingly, the number of oligodendrocytes progressively reduced within 24 h after injury. Administration of oligodendrocyte-like cells (OLCs) into the lesion area is one of the approaches to counterbalance this condition. Methods: Bone marrow stromal cells were transdifferentiated into neurospheres and then into neural stem cells and later were differentiated into OLCs using triiodothyronine and transplanted into the spinal cord contusion rats. The postinjury functional recovery was explored and compared with the control group using Basso-Beattie-Bresnahan and narrow beam behavioral tests. At the end of 12 th week, spinal cord segments T12-L1 were histomorphologically studied by immunohistochemistry. Results: Motor improvement was more obvious during 2 nd to 4 th weeks and got less prominent during 4 th to 12 th weeks. Histomorphometric findings indicated that cavity formation decreased in epicenter of transplantation area in experimental groups in comparison with the control groups. Conclusion: The findings obtained in the present study showed that OLC therapy is a potential approach in the treatment of spinal cord traumatic injuries.

show abstract

Section: Surgical Proceduresmentioning

confidence: 99%

Decrease in Cavity Size and Oligodendrocyte Cell Death Using Neurosphere-Derived Oligodendrocyte-Like Cells in Spinal Cord Contusion Model

Abbaszadeh

Tiraihi

Sadeghi

et al. 2018

IBJ

Self Cite

View full text Add to dashboard Cite

show abstract

“…To exert trophic activity, PDGFRα is required. PDGFRα activation promotes OPC proliferation, but it is thought to inhibit OPC differentiation into mature oligodendrocytes (Abbaszadeh et al, 2014). NG2, another OPC marker, also regulates PDGF action (Asher et al, 2005).…”

Section: Factors Affecting Remyelination After Strokementioning

confidence: 99%

Mechanisms of cell–cell interaction in oligodendrogenesis and remyelination after stroke

et al. 2015

View full text Add to dashboard Cite

White matter damage is a clinically important aspect of several central nervous system diseases, including stroke. Cerebral white matter primarily consists of axonal bundles ensheathed with myelin secreted by mature oligodendrocytes, which play an important role in neurotransmission between different areas of gray matter. During the acute phase of stroke, damage to oligodendrocytes leads to white matter dysfunction through the loss of myelin. On the contrary, during the chronic phase, white matter components promote an environment, which is favorable for neural repair, vascular remodeling, and remyelination. For effective remyelination to take place, oligodendrocyte precursor cells (OPCs) play critical roles by proliferating and differentiating into mature oligodendrocytes, which help to decrease the burden of axonal injury. Notably, other types of cells contribute to these OPC responses under the ischemic conditions. This mini-review summarizes the non-cell autonomous mechanisms in oligodendrogenesis and remyelination after white matter damage, focusing on how OPCs receive support from their neighboring cells.

show abstract

“…The ADMSCs were plated in Fibrin coated TCPS and bare TCPS at a density of 10,000 cells/cm 2 in DMEM/F12 basal media, with 10% Foetal bovine serum (FBS) and 1% AB/AM. After 24 hours, the basal media supplemented with 1% FBS, 1% AB/AM, 20 ng/ml basic fibroblast growth factor (bFGF) and 20 ng/ml epidermal growth factor (EGF) was added to the cells in bare TCPS (INB) and in niche (INF) (Abbaszadeh et al 39 ). 10% FBS and 1% AB/AM supplemented basal media was added to the control group, the un-induced ADMSC in fibrin.…”

Section: Methodsmentioning

confidence: 99%

“…The induced NPCs were further supplemented with 10 ng/ml PDGF-AA and bFGF in basal medium (1% FBS) for OPC induction. (Modified protocol published by Abbaszadeh et al 39 ). The culture was terminated in another 4 days to evaluate the differentiation using ICC, qRT-PCR, and flow cytometry.…”

Section: Neural and Glial Progenitor Inductions Adherent Ns Formed Fmentioning

confidence: 99%

Exploitation of fibrin-based signaling niche for deriving progenitors from human adipose-derived mesenchymal stem cells towards potential neural engineering applications

Chandrababu

Senan

Krishnan

2020

Sci Rep

View full text Add to dashboard Cite

Adipose-derived mesenchymal stem cells (hADMSC) retaining proliferation and multi-differentiation potential may support the central nervous system (CNS) regeneration. Multipotency of MSC may result in both desirable and undesirable cells, post-transplantation. A better strategy to attain desired cells may be in vitro commitment of hADMSCs to uni-/bi-potent neural progenitor cells (NPCs), prior to transplantation. Derivation of stable NPCs may require a suitable niche eliciting proliferation and differentiation signals. The present study designed a biomimetic niche comprising insoluble fibrin supported adhesion matrix and exogenously added growth factors (GFs) for deriving different neural cells and established the role of Notch and Wnt signals for proliferation and differentiation of hADMSCs, respectively. The stable transformation of hADMSCs into neurospheres (NS) comprising Nestin +ve NPCs was achieved consistently. Slight modifications of niche enable differentiation of NS to NPCs; NPCs to neurons; NPCs to oligodendrocyte progenitor cells (OPCs); and OPCs to oligodendrocytes (OLG). Fibrin plays a crucial role in the conversion of hADMSC to NS and NPCs to OPCs; but, not essential for OPC to OLG maturation. Co-survival and cell-cell interaction of NPC derived neurons and OPCs promoting OLG maturation is illustrated. The designed biomimetic niche shows the potential for directing autologous ADMSCs to neural cells for applications in regenerative medicine.The injured central nervous system (CNS) tissue shows limited and slothful ability to regenerate. This may be because of the inadequate number of endogenous neural progenitor cells (NPC) and the development of unfavorable micro-environment post-injury, affecting cell homing and differentiation. The stem cell transplantation is considered as a prospective therapy for CNS injury, considering the trophic support and its potential to differentiate into specific cells in the injured region 3 . The injury-associated and other degenerating diseases affecting CNS require glial and neuronal cells for its regeneration into fully functional tissue. Therefore, the exploitation of multi-potent hADMSCs may aim generation of NPCs and oligodendrocyte progenitor cell (OPC) with proliferation/differentiation potential starting from the same source of hADMSC, for mixed cell transplantation. Differentiation into each cell type may require specific niche conditions consisting of adhesive protein and growth factors (GF).Several advantages of the fibrin-based niche in stem cell growth and differentiation have been reported. Polymerized fibrin network constitutes several other adhesive proteins including fibronectin(FN) and laminin(La) playing a prominent role in eliciting signals for proliferation, survival, and differentiation. A previous study reported that both FN and La are responsible for stimulating Nestin +ve progenitors in peripheral blood mononuclear cells (PBMNC) directing differentiation to neurons 4,5 . The heparin-binding domains of the FN can immobilize GFs like PDGF, FGF...

show abstract

Differentiation of neurosphere-derived rat neural stem cells into oligodendrocyte-like cells by repressing PDGF-α and Olig2 with triiodothyronine

Cited by 21 publications

References 44 publications

Decrease in Cavity Size and Oligodendrocyte Cell Death Using Neurosphere-Derived Oligodendrocyte-Like Cells in Spinal Cord Contusion Model

Decrease in Cavity Size and Oligodendrocyte Cell Death Using Neurosphere-Derived Oligodendrocyte-Like Cells in Spinal Cord Contusion Model

Mechanisms of cell–cell interaction in oligodendrogenesis and remyelination after stroke

Exploitation of fibrin-based signaling niche for deriving progenitors from human adipose-derived mesenchymal stem cells towards potential neural engineering applications

Contact Info

Product

Resources

About