Differentiation of Myxobolus spp. (Myxozoa: Myxobolidae) infecting roach (Rutilus rutilus) in Hungary

Abstract

During a survey on fishes from Hungarian freshwaters, the occurrence of eight Myxobolus species was registered in roach (Rutilus rutilus L.). Most species had a specific location within the fish host. Of the known species infecting roach, the intramuscularly developing species Myxobolus pseudodispar was found to be the most common. Besides Myxobolus intimus and Myxobolus diversicapsularis infecting the capillary network of gill lamellae, Myxobolus feisti infecting the cartilaginous gill rays were observed. Of the "muelleri-type" Myxobolus spp, Myxobolus rutili and Myxobolus sommervillae sp. n. formed elongated plasmodia in the gill filaments outside and inside the arteria efferens, respectively, while Myxobolus wootteni sp. n. developed cysts in fins. Plasmodia and spores of Myxobolus fundamentalis sp. n. were detected in the cartilaginous gill arch under the basis of gill filaments. Despite similarities of some species in spore morphology, 18S rDNA sequences and phylogenetic analyses showed clear differences among all species examined. The findings of the study demonstrate that morphologically similar spores could only be correctly identified by considering the location of plasmodia and the genetic characters of the myxozoan species.

Morphological and molecular characterization of Myxobolus mucosus sp. n. (Myxosporea: Myxobolidae) with basifilamental sporulation in two cyprinid fishes, Rutilus rutilus (L.) and Leuciscus leuciscus (L.) in Russia

“…Roach, Rutilus rutilus (L.), and common dace, Leuciscus leuciscus (L.), are two of the most common cyprinid fish in Europe where the myxosporean fauna of these two fishes have been extensively studied (Eiras et al 2005(Eiras et al , 2014Molnár et al 2010). About 24 and 15 Myxobolus species have been respectively reported from roach and common dace in Russia, among which 10 species were recorded from both of these two hosts (Donec and Shulman 1984; Eiras et al 2005).…”

“…Thus, a comprehensive strategy including spore morphology, exact location of sporulation, tissue specificity, host specificity, and molecular data was widely thought to be essential for differentiation of the large number of species with morphological similarity. Additionally, some new Myxobolus species with specific habitat have been recently reported in roach in Hungary (Molnár et al 2006(Molnár et al , 2008(Molnár et al , 2010. To further correctly uncover the myxosporean diversity of freshwater fish in Finish Bay by applying the late taxonomic criteria, we performed a myxosporean fauna investigation of three consecutive years (2012)(2013)(2014).…”

Molecular characterization of Sphaerospora molnari (Myxozoa), the agent of gill sphaerosporosis in common carp Cyprinus carpio carpio

“…have become 'mainstream' in myxozoan research (e.g. Whipps et al 2003, Eszterbauer 2004, Holzer et al 2004, Fiala 2006, Molnár et al 2010, Fiala & Bartošová 2010. Due to the unknown host specificity and geographical distribution of many myxozoan taxa, it is important to provide a combination of morphological and molecular features for a given myxozoan species.…”

Redescription of Henneguya chaudhuryi (Bajpai & Haldar, 1982) (Myxosporea: Myxobolidae), infecting the gills of the freshwater fish Channa punctata (Bloch) (Perciformes: Channidae) in India

“…Accurate identification of these parasites in India is problematic due to the typically poor morphological descriptions and the lack of molecular data. However, recent publications (Molnár et al, 2010;Liu et al, 2012;Székely et al, 2015) suggest that molecular approaches are necessary for correct species identification. In India, molecular tools have barely been applied in studies…”