Differentiation of monocytes into macrophages induces the upregulation of histamine H1 receptor

Triggiani, Massimo; Petraroli, Angelica; Loffredo, Stefania; Frattini, Annunziata; Granata, Francescopaolo; Morabito, Paolo; Staiano, Rosaria Ilaria; Secondo, Agnese; Annunziato, Lucio; Marone, Gianni

doi:10.1016/j.jaci.2006.09.027

Cited by 58 publications

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“…1). In agreement with our data, Triggiani et al (2007) reported that HA did not increase [Ca 21 ] i in human monocytes, whereas a reference stimulus did. Dijkstra et al (2007) reported that 5-MHA and clobenpropit, another H 4 R agonist ] i were assessed.…”

Section: Discussionsupporting

confidence: 93%

No Evidence for Histamine H₄Receptor in Human Monocytes

Werner

Neumann

Buschauer

et al. 2014

J Pharmacol Exp Ther

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The histamine H 4 receptor (H 4 R) is a classic pertussis toxinsensitive G i protein-coupled receptor that mediates increases in intracellular calcium concentration ([Ca 21 ] i ). The presence of H 4 R in human eosinophils has been rigorously documented by several independent groups. It has also been suggested that H 4 R is expressed in human monocytes, but this suggestion hinges in part on H 4 R antibodies with questionable specificity. This situation prompted us to reinvestigate H 4 R expression in human monocytes. As positive control, we studied human embryonic kidney 293T cells stably expressing the human H 4 R (hH 4 R). In these cells, histamine (HA) and the H 4 R agonistHowever, in quantitative real-time polymerase chain reaction studies we failed to detect hH 4 R mRNA in human monocytes and U937 promonocytes. In human monocytes, ATP and] i , but HA, UR-PI376, and 5-methylhistamine (a dual H 4 R/H 2 receptor agonist) did not. In U937 promonocytes and differentiated U937 cells, HA increased [Ca 21 ] i , but this increase was mediated via HA H 1 receptor. In conclusion, there is no evidence for the presence of H 4 R in human monocytes.

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Section: Discussionsupporting

confidence: 93%

No Evidence for Histamine H₄Receptor in Human Monocytes

Werner

Neumann

Buschauer

et al. 2014

J Pharmacol Exp Ther

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“…However, the observation that one such form is expressed in macrophages but not in monocytes indicates that its synthesis is selectively activated during differentiation and suggests that it may play a role in specific macrophage functions. Induction or upregulation of gene transcription occurs when monocytes differentiate into macrophages [20,21,49]. Thus, it is conceivable that final differentiation of tissue macrophages is associated with upregulation of NCX1 and NCX3 and with induction of truncated NCX1.…”

Section: Discussionmentioning

confidence: 99%

“…Reverse transcription was done as described previously [49]. cDNA was then titrated for GAPDH message and the expression of NCX1, NCX2, and NCX3 was evaluated by semiquantitative PCR using targetspecific primers designed according to the published sequences (GenBank accession nos.…”

Section: Rt-pcr For Ncx1 Ncx2 and Ncx3mentioning

confidence: 99%

“…2009. 39: 1405-1418 Leukocyte signaling as assessed by flow cytometry analysis and a-naphthylacetate esterase staining [49]. In the experiments with freshly isolated macrophages, the enriched macrophage suspension was layered onto additional Percolls gradients to reach purity between 95 and 99%, as assessed by CD14/CD71/HLA-DR staining with flow cytometry.…”

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confidence: 99%

“…PBMC were obtained by centrifugation over Histopaques-1077. Monocytes were purified from PBMC by negative immunomagnetic selection (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), as described previously [49]. This procedure yields a population of monocytes with purity 497%, assessed by flow cytometry analysis of CD14.…”

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Expression and function of Na⁺/Ca²⁺ exchangers 1 and 3 in human macrophages and monocytes

et al. 2009

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The Na 1 /Ca 21 exchanger (NCX) is a membrane transporter that can switch Na 1 and Ca 21 in either direction to maintain the homeostasis of intracellular Ca 21 . Three isoforms (NCX1, NCX2, and NCX3) have been characterized in excitable cells, e.g. neurons and muscle cells. We examined the expression of these NCX isoforms in primary human lung macrophages (HLM) and blood monocytes. NCX1 and NCX3, but not NCX2, are expressed in HLM and monocytes at both mRNA and protein levels. Na 1 -free medium induced a significant increase in intracellular calcium concentration ([Ca 21 ] i ) in both cell types. This response was completely abolished by the NCX inhibitor 5-(N-4-chlorobenzyl)-20,40-dimethylbenzamil (CB-DMB). Moreover, inhibition of NCX activity during Ca 21 -signaling induced by histamine caused a delay in restoring baseline [Ca 21 ] i . Na 1 -free medium induced TNF-a expression and release in HLM comparable to that caused by LPS. TNF-a release induced by Na 1 -free medium was blocked by CB-DMB and greatly reduced by RNAi-mediated knockdown of NCX1. These results indicate that human macrophages and monocytes express NCX1 and NCX3 that operate in a bidirectional manner to restore [Ca 21 ] i , to generate Ca 21 -signals, and to induce TNF-a production. Therefore, NCX may contribute to regulate Ca 21 homeostasis and proinflammatory functions in human macrophages and monocytes.Key words: Ca 21 -signaling . Macrophages . Monocytes . Na 1 /Ca 21 exchanger IntroductionThe Na 1 /Ca 21 exchanger (NCX) is a plasma membrane protein involved in the homeostasis of intracellular Ca 21 [1]. NCX is a bidirectional ion transporter that catalyzes the exchange of Na 1 and Ca 21 depending on their electrochemical gradients [2]. Under physiological conditions, NCX's primary role is to extrude Ca 21 from cells using the Na 1 gradient across the cell membrane (forward mode of operation) [3]. However, in some cases it can contribute to the Ca 21 influx into the cells by working in the reverse mode (i.e. coupling Ca 21 influx with Na 1 efflux) [4][5][6].Three isoforms of NCX, NCX1, NCX2, and NCX3 have been cloned in mammals, outlining a multigene family whose members share a similar molecular structure [7]. NCX is modeled with nine transmembrane segments involved in ion transport and a long central cytoplasmic loop that has regulatory functions [8]. However, truncated forms lacking certain transmembrane segments, but still possessing NCX activity, have also been Eur. J. Immunol. 2009. 39: 1405-1418 DOI 10.1002 Leukocyte signaling 1405 described as the result of alternative splicing of the three genes [9][10][11]. NCX1 is largely predominant in mammalian tissues such as heart, brain, and kidney while the expression of NCX2 and NCX3 appears to be limited to the brain and skeletal muscle [7]. NCX is a key element regulating intracellular Ca 21 concentrations ([Ca 21 ] i ) in many excitable cells, including myocardiocytes, retinal rod photoreceptors, smooth muscle cells, and neurons [3]. Non-excitable cells such as renal epithelial ce...

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Influence of Histamine Receptor Antagonists on the Outcome of Perforated Appendicitis

Peter¹

2010

Arch Surg

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Differentiation of monocytes into macrophages induces the upregulation of histamine H1 receptor

Cited by 58 publications

References 34 publications

No Evidence for Histamine H₄Receptor in Human Monocytes

No Evidence for Histamine H₄Receptor in Human Monocytes

Expression and function of Na⁺/Ca²⁺ exchangers 1 and 3 in human macrophages and monocytes

Influence of Histamine Receptor Antagonists on the Outcome of Perforated Appendicitis

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Differentiation of monocytes into macrophages induces the upregulation of histamine H1 receptor

Cited by 58 publications

References 34 publications

No Evidence for Histamine H4Receptor in Human Monocytes

No Evidence for Histamine H4Receptor in Human Monocytes

Expression and function of Na+/Ca2+ exchangers 1 and 3 in human macrophages and monocytes

Influence of Histamine Receptor Antagonists on the Outcome of Perforated Appendicitis

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No Evidence for Histamine H₄Receptor in Human Monocytes

No Evidence for Histamine H₄Receptor in Human Monocytes

Expression and function of Na⁺/Ca²⁺ exchangers 1 and 3 in human macrophages and monocytes