1999
DOI: 10.1523/jneurosci.19-21-09445.1999
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Differentiation of Mammalian Vestibular Hair Cells from Conditionally Immortal, Postnatal Supporting Cells

Abstract: We provide evidence from a newly established, conditionally immortal cell line (UB/UE-1) that vestibular supporting cells from the mammalian inner ear can differentiate postnatally into more than one variant of hair cell. A clonal supporting cell line was established from pure utricular sensory epithelia of H2k(b)tsA58 transgenic mice 2 d after birth. Cell proliferation was dependent on conditional expression of the immortalizing gene, the "T" antigen from the SV40 virus. Proliferating cells expressed cytokera… Show more

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Cited by 48 publications
(40 citation statements)
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References 59 publications
(63 reference statements)
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“…Last, we suggest that replication of the physiological profile through which embryonic hair cells develop (Fig. 9) in candidate regenerated hair cells (Lawlor et al, 1999;Shou et al, 2003) will help validate their advancement to the enviable status of fully functional hair cell.…”
Section: Discussionmentioning
confidence: 86%
“…Last, we suggest that replication of the physiological profile through which embryonic hair cells develop (Fig. 9) in candidate regenerated hair cells (Lawlor et al, 1999;Shou et al, 2003) will help validate their advancement to the enviable status of fully functional hair cell.…”
Section: Discussionmentioning
confidence: 86%
“…Oshima et al 2007;Savary et al 2007Savary et al , 2008Senn et al 2007). To identify differentiated cells, we used antibodies specific for the early hair cell markers myosin VIIa ) and parvalbumin 3 (Heller et al 2002), as well as antibodies to pan-cytokeratin, which label epithelial cells including supporting cells (Lawlor et al 1999;Li et al 2003;Zheng et al 1997). The morphology and the pattern of immunoreactivity for pan-cytokeratin after 5 days (Fig.…”
Section: Figmentioning
confidence: 99%
“…Similarly, it is unlikely that screens utilizing in vitro preparations of utricles or cochleae from larger animals will be routinely adopted, as thousands of animals would be required to screen even mediumsized compound libraries. Hair cell lines (Rivolta et al 1998;Lawlor et al 1999) provide an alternative model that is more compatible for screening; however, to date, no screen of ototoxicity in the few lines available has been reported. Furthermore, the use of hair cell lines is controversial because these cells have been selected to survive and thus may not be an ideal model to screen for hair cell death.…”
Section: Alternative Approaches To Screeningmentioning
confidence: 99%