Differentiation of lung stem/progenitor cells into alveolar pneumocytes and induction of angiogenesis within a 3D gelatin – Microbubble scaffold

Ling, Thai-Yen; Liu, Yen-Liang; Huang, Yao-Hsien; Gu, Sing-Yi; Chen, Hung-Kuan; Ho, Choa-Chi; Tsao, Po‐Nien; Tung, Yi-Chung; Chen, Huei‐Wen; Cheng, Chiung-Hsiang; Lin, Keng-hui; Lin, Feng‐Huei

doi:10.1016/j.biomaterials.2014.03.074

Cited by 46 publications

(37 citation statements)

References 41 publications

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“…For example, the functional role of hypoxia in vascular flow was nicely explored in the studies published by Lindgren and colleagues (128). Initially, the CAM performs gas-exchange functions via the area vasculosa, the main respiratory organ before day 6 [173], but this is gradually substituted by the CAM vessels. By day 14, the CAM capillary network is completely developed and by day 19 the embryonic lung ventilation starts as the CAM degenerates and the embryo hatches.…”

Section: Cam As a Screening Platformmentioning

confidence: 99%

“…The CAM has been also used in stem cell research in which various extracellular matrices or synthetic polymer-derived scaffolds have been evaluated in an effort to construct desired tissue-like structures [173,198] [176]. The combination of biocompatible materials and stem cells appears to be a future trend that might prove fruitful in comparison to other systems, in large part, due to the neovascular properties of the CAM.…”

Section: Cam As a Screening Platformmentioning

confidence: 99%

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The chicken chorioallantoic membrane model in biology, medicine and bioengineering

2014

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The chicken chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development, including but not restricted to gas exchange. Over the last two decades, interest in the CAM as a robust experimental platform to study blood vessels has been shared by specialists working in bioengineering, development, morphology, biochemistry, transplant biology, cancer research and drug development. The tissue composition and accessibility of the CAM for experimental manipulation, makes it an attractive preclinical in vivo model for drug screening and / or for studies of vascular growth. In this article we provide a detailed review of the use of the CAM to study vascular biology and response of blood vessels to a variety of agonists. We also present distinct cultivation protocols discussing their advantages and limitations and provide a summarized update on the use of the CAM in vascular imaging, drug delivery, pharmacokinetics and toxicology.

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Section: Cam As a Screening Platformmentioning

confidence: 99%

Section: Cam As a Screening Platformmentioning

confidence: 99%

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

2014

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“…Another aspect of lung bioengineering is the development of ambulatory lung assist devices using microfluidic vascular networks to mimic alveolar-capillary membranes[11–15]. Another miniaturized device using stacked, microfluidic oxygenators units may have potential to treat respiratory-insufficient newborns and is currently being tested in piglets[16].…”

Section: Resultsmentioning

confidence: 99%

Bioreactor Development for Lung Tissue Engineering

Panoskaltsis‐Mortari

2015

Curr Transpl Rep

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Rationale Much recent interest in lung bioengineering by pulmonary investigators, industry and the organ transplant field has seen a rapid growth of bioreactor development ranging from the microfluidic scale to the human-sized whole lung systems. A comprehension of the findings from these models is needed to provide the basis for further bioreactor development. Objective The goal was to comprehensively review the current state of bioreactor development for the lung. Methods A search using PubMed was done for published, peer-reviewed papers using the keywords “lung” AND “bioreactor” or “bioengineering” or “tissue engineering” or “ex vivo perfusion”. Main Results Many new bioreactors ranging from the microfluidic scale to the human-sized whole lung systems have been developed by both academic and commercial entities. Microfluidic, lung-mimic and lung slice cultures have the advantages of cost-efficiency and high throughput analyses ideal for pharmaceutical and toxicity studies. Perfused/ventilated rodent whole lung systems can be adapted for mid-throughput studies of lung stem/progenitor cell development, cell behavior, understanding and treating lung injury and for preliminary work that can be translated to human lung bioengineering. Human-sized ex vivo whole lung bioreactors incorporating perfusion and ventilation are amenable to automation and have been used for whole lung decellularization and recellularization. Clinical scale ex vivo lung perfusion systems have been developed for lung preservation and reconditioning and are currently being evaluated in clinical trials. Conclusions Significant advances in bioreactors for lung engineering have been made at both the microfluidic and the macro scale. The most advanced are closed systems that incorporate pressure-controlled perfusion and ventilation and are amenable to automation. Ex vivo lung perfusion systems have advanced to clinical trials for lung preservation and reconditioning. The biggest challenges that lie ahead for lung bioengineering can only be overcome by future advances in technology that solve the problems of cell production and tissue incorporation.

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“…Additionally, gelatin has been used in numerous human applications, which makes it an appealing polymer for new tissue engineered products. Gelatin in the form of a three-dimensional microbubble scaffold was used to provide the proper cellular microenvironment for differentiation of mouse pulmonary stem cells into alveolar pneumocytes [140]. Similarly, hydroxyethyl methacrylate-alginate-gelatin (HAG) hydrogels have been employed as three-dimensional structures for lung epithelial cell growth [141].…”

Section: Polymer Sponges or Hydrogels For Structured Cellular Transplmentioning

confidence: 99%

Polymers in Tissue Engineering

Heise

Blakeney

Pouliot

2014

Advanced Polymers in Medicine

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The landscape of polymer selection and processing techniques is constantly evolving in the field of tissue engineering and regenerative medicine. This chapter will cover new advances in polymers that are used to regenerate functional tissues used to repair or replace tissues lost to age, disease, injury, or congenital defect. The focus will be on new processing techniques and the incorporation of biologics or drug delivery to enhance cellular response and ingrowth into the polymers that will create a more functional tissue replacement by engineering the polymer tissue interface. Special emphasis is placed on new frontiers in tissue engineering the lung and liver.

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Differentiation of lung stem/progenitor cells into alveolar pneumocytes and induction of angiogenesis within a 3D gelatin – Microbubble scaffold

Cited by 46 publications

References 41 publications

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

Bioreactor Development for Lung Tissue Engineering

Polymers in Tissue Engineering

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