Differentiation of isomeric methylanilines by imidization and gas chromatography/mass spectrometry analysis

Chen, Mengmeng; Zhu, Guohua; Xu, Jiaxiang; Zhang, Huarong; Liu, Jinsong; Jiang, Kezhi

doi:10.1002/rcm.8043

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…These technologies enable the characterization of various molecules, such as DNA (scDNA-seq) 6 , RNA (scRNA-seq) 7,8 , and proteins 9 . They can also facilitate epigenetic studies through single-cell ATAC sequencing (scATAC-seq) 10,11 and methylation 12,13 . There have also been rapid developments of spatial single-cell technologies 14 .…”

Section: Introductionmentioning

confidence: 99%

CosGeneGate Selects Multi-functional and Credible Biomarkers for Single-cell Analysis

Liu,

Long,

Cao

et al. 2024

Preprint

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Selecting representative genes or marker genes to distinguish cell types is an important task in single-cell sequencing analysis. Although many methods have been proposed to select marker genes, the genes selected may have redundancy and/or do not show cell-type-specific expression patterns to distinguish cell types. Here we present a novel model, named CosGeneGate, to select marker genes for more effective marker selections. CosGeneGate is inspired by combining the advantages of selecting marker genes based on both cell-type classification accuracy and marker gene specific expression patterns. We demonstrate the better performance of the marker genes selected by CosGeneGate for various downstream analyses than the existing methods with both public datasets and newly sequenced datasets. The non-redundant marker genes identified by CosGeneGate for major cell types and tissues in human can be found at the website as follows: https://github.com/VivLon/CosGeneGate/blob/main/marker gene list.xlsx.

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Section: Introductionmentioning

confidence: 99%

CosGeneGate Selects Multi-functional and Credible Biomarkers for Single-cell Analysis

Liu,

Long,

Cao

et al. 2024

Preprint

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“…Aromatic amines are extensively used as raw materials or as intermediates in the manufacturing of industrial chemicals such as pharmaceuticals, agrochemicals, polymers, azo dyes, and rubbers. − Aromatic anilines are classified as an important class of environmental pollutants because they are highly toxic to humans and are potential carcinogens. − Due to their high solubility in water, they are easy to penetrate into soil and pollute groundwater, where they have been found at trace levels (<10 μg/L). − For this reason, in this last period, an increasing attention has been devoted to the development of new, reliable, highly selective, sensitive, and rapid analytical methods for their detection, leading to the publication of numerous reports in this field, − exploiting in particular gas chromatography (GC) techniques. − However, direct determination of aromatic aniline isomers using GC techniques is still difficult due to their polar nature; then, aromatic aniline isomers are usually first derivatized and then determined by GC in combination with mass spectrometry (MS) techniques. − Derivatization greatly increases the analysis time and its cost, and it makes use of great amounts of organic solvents, becoming a source of environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Separation of Aromatic Amine Isomers: A Solved Issue by a New Amphiphilic Pillar[6]arene Stationary Phase

Sun

Chen

Huang

et al. 2022

ACS Appl. Mater. Interfaces

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In this work, the fabrication, synthesis, and characterization of a new stationary phase based on an amphiphilic pillar[6]arene (P6A-C10-2NH2) for gas chromatographic analyses are reported. The gas chromatography (GC) column prepared with P6A-C10-2NH2 stationary phase exhibited a medium polarity, an efficiency of 3219 plates/m, and unmatched resolving capabilities toward chloroaniline, bromoaniline, iodoaniline, and toluidine isomers. Furthermore, the P6A-C10-2NH2 column showed excellent repeatability with maximum relative standard deviations equal to 0.02, 0.07, and 2.56% for run-to-run, day-to-day, and column-to-column, respectively, demonstrating a great potential as a new stationary phase in separation science.

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“…Chemical derivatization changes the physicochemical properties of an analyte by introducing a modifying group into its certain functional groups, which can improve the detection sensitivity of the analyte, and exclude the matrix interference in samples 14–17 . Chemical derivatization increases the stability and the volatility of the sample, and improves the capacity of GC separation, which has been applied for the analysis of polar compounds by GC/mass spectrometry (MS) 18–21 . Guo's group has successfully converted the inorganic, nonvolatile boric acid into a volatile triethanolamine borate, and then developed a fast, selective and sensitive method for the determination of the inorganic boric acid in drinking water by GC/MS analysis 22 .…”

Section: Introductionmentioning

confidence: 99%

Determination of trace fluoride in water samples by silylation and gas chromatography/mass spectrometry analysis

Chen

Wang

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale Due to the ubiquity of fluorides and the small gap between a safe dose and a harmful one, it is necessary to develop a robust analytical method for determination of fluoride ions in various water samples with complex matrices. Methods Silylation of the fluoride ion was carried out by treatment with hydrochloric acid and phenyldimethylchlorosilane at room temperature. The formed phenyldimethylfluorosilane was detected by gas chromatography/mass spectrometry (GC/MS). Results Under the optimized conditions, linearity in the analytical method ranged from 0.050 to 5.0 μg/mL for the fluoride ion with R2 >0.9999. Reasonable reproducibility was obtained with the intraday relative standard deviation (RSD) (N = 5) of 2.04% and interday RSD (N = 5) of 3.75% at the concentration of 0.10 μg/mL. The developed method has been successfully applied to determine the fluoride ion in real water samples, including waste water samples, with the recovery between 81.12% and 113.04%. Conclusions A robust method for the determination of the fluoride ion has been developed by silylation with phenyldimethylchlorosilane and GC/MS analysis. The established method showed good anti‐interference and precision, and it has been applied for determination of the fluoride ion in various water samples.

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Differentiation of isomeric methylanilines by imidization and gas chromatography/mass spectrometry analysis

Abstract: These results provided a promising solution for simultaneous determination of isomeric methylanilines.

Cited by 4 publications

References 23 publications

CosGeneGate Selects Multi-functional and Credible Biomarkers for Single-cell Analysis

CosGeneGate Selects Multi-functional and Credible Biomarkers for Single-cell Analysis

Chromatographic Separation of Aromatic Amine Isomers: A Solved Issue by a New Amphiphilic Pillar[6]arene Stationary Phase

Determination of trace fluoride in water samples by silylation and gas chromatography/mass spectrometry analysis

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