Differentiation of <i>Candida</i> strains by lectin‐mediated agglutination kinetics

Nenoff, P.; Süss, Karl-Heinz; Flemming, C; Haustein, U.‐F.

doi:10.1046/j.1439-0507.2000.00554.x

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…We conclude that the lectin-binding assay presented in this paper eliminates many of the laborious steps involved in other protocols 16,18,19,[24][25][26][27] and is reproducible. Some advantages are the amount and quality of the mycelium and the fact the spores immobilised on the adhesive tapes were suitable for accessing the carbohydrate profile in glycoconjugates of the cell wall surface of filamentous fungi isolated from public park soils in the city of Recife, Pernambuco state, Northeast Brazil.…”

Section: Discussionmentioning

confidence: 70%

Carbohydrate profiling of fungal cell wall surface glycoconjugates of Trichophyton tonsurans and other keratinophilic filamentous fungi using lectins

Leal

Lima-Neto

Macêdo

et al. 2011

Mycoses

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Various researchers have concluded that lectins are useful reagents for the study of fungal cell wall surface glycoconjugates. In this study, we evaluated the expression of N-acetyl-D-glucosamine, L-fucose, D-galactose and glucose/mannose on the cell wall surface of Trichophyton tonsurans and other keratinophilic filamentous fungi, using a simple lectin-binding protocol. The fungal cultures used were isolated from soils obtained from public parks by the hair-bait technique. The lectin assays used concanavalin A (Con A), wheat germ agglutinin (WGA), Ulex europeus agglutinin I (UEA-I) and peanut agglutinin (PNA), all conjugated with horseradish peroxidase. Adhesive tape was placed sticky-side down over the fungal colony, gently pressed and then removed. The fungal-tape samples were incubated with the lectin for 1 h at 4 °C. Lectin binding was visualised using 3,3-diaminobendizine (DAB) and hydrogen peroxidase. There was a high expression of N-acetyl-D-glucosamine on the cell wall surface of all fungi species tested, whereas the expression of L-fucose, D-galactose and glucose/mannose demonstrated inter-specific variations. The lectin-binding assay presented in this article eliminates many of the laborious steps involved in other protocols. The amount and quality of the mycelium and spores immobilised by the adhesive tapes were suitable for obtaining the carbohydrate profile in glycoconjugates of the cell wall surface of filamentous fungi.

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Section: Discussionmentioning

confidence: 70%

Carbohydrate profiling of fungal cell wall surface glycoconjugates of Trichophyton tonsurans and other keratinophilic filamentous fungi using lectins

Leal

Lima-Neto

Macêdo

et al. 2011

Mycoses

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2000

Yeast

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2‐Methyl‐6‐arylimidazo[1,2‐a]pyrazin‐3(7H)‐ones with a substituent such as phenyl, 4‐methoxyphenyl or 4‐trifluoromethoxyphenyl at the 6‐position of the imidazo[1,2‐a]pyrazin‐3(7H)‐one ring system, produced chemiluminescence emission in mixtures of water and DMF and in several mixtures of MeOH and DMF under neutral conditions. Under these protic luminescence conditions, the respective light emissions were generated from neutral singlet excited‐state molecules. The electron‐donating effect of the 4‐methoxy substituent on the phenyl group increased the efficiency of the neutral singlet excited state formation, whereas non‐substitution and a 4‐trifluoromethoxy group having no electron donating ability decreased the efficiency. The compound having the electron‐donating methoxy group substituent showed two chemiluminescence emitters, which generated light at λmax 410–420 nm and 460 nm. It was determined that the neutral molecules in the excited state generating light emission at the shorter wavelengths are neutral singlet excited‐state molecules suitable for highly efficient singlet excited‐state formation. A role of the electron‐donating effect of the methoxy group is postulated to be generation of the special neutral singlet excited‐state molecules. Copyright © 1999 John Wiley & Sons, Ltd.

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Carbohydrate profiling of fungal cell wall surface glycoconjugates ofAspergillusspecies in brain and lung tissues using lectin histochemistry

Leal¹,

Lopes²,

Clark

et al. 2012

Med Mycol

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The aim of this study was to evaluate, through lectin histochemistry, the expression of N-acetyl-D-glucosamine, L-fucose, D-galactose and glucose/mannose on the cell wall surfaces of Aspergillus species in histopathological specimens of brain (n = 1) and lung (n = 6) tissues obtained during autopsy of patients diagnosed postmortem as having had invasive aspergillosis. Concanavalin A (Con A), wheat germ agglutinin (WGA), Ulex europeus agglutinin I (UEA-I) and peanut agglutinin (PNA), all conjugated with horseradish peroxidase, were employed. Lectin-binding was visualized using 3,3-diaminobendizine (DAB) and hydrogen peroxide in phosphate buffer solution (PBS). We observed expression of N-acetyl-D-glucosamine and methyl-α-D-mannoside on the cell wall surfaces of all evaluated Aspergillus species, while the expression of L-fucose and D-galactose demonstrated inter and intra-specific variations. The results obtained from this study indicate that the use of WGA and Con A lectins permits visualization of Aspergillus structures such as hyphae, conidial heads and conidia in histopathological specimens of brain and lung tissues.

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Differentiation of Candida strains by lectin‐mediated agglutination kinetics

Cited by 4 publications

References 27 publications

Carbohydrate profiling of fungal cell wall surface glycoconjugates of Trichophyton tonsurans and other keratinophilic filamentous fungi using lectins

Carbohydrate profiling of fungal cell wall surface glycoconjugates of Trichophyton tonsurans and other keratinophilic filamentous fungi using lectins

Current Awareness

Carbohydrate profiling of fungal cell wall surface glycoconjugates ofAspergillusspecies in brain and lung tissues using lectin histochemistry

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