Differentiation of Human Wharton's Jelly Cells Toward Nucleus Pulposus-Like Cells After Coculture with Nucleus Pulposus Cells <i>In Vitro</i>

Ruan, Dike; Zhang, Yan; Wang, DeLi; Zhang, Chao; Wu, Jianhong; Wang, Chaofeng; Shi, Zhiyuan; Xin, Hongkui; Xu, Chen; Li, Haifeng; He, Qiang

doi:10.1089/ten.tea.2011.0186

Cited by 31 publications

(29 citation statements)

References 43 publications

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“…1B), indicating that WJ-MSC had not gone into senescence. The results are consistent with previous reports that WJ-MSC requires a greater number of passages in culture to enter senescence, and can retain stemness properties for a longer period time in vitro 31-33. On the other hand, culturing BM-MSC from passage 5 to 11 resulted in the cells displaying senescence characteristics in being enlarged and flat in shape (Fig.…”

Section: Resultssupporting

confidence: 92%

Oxidative Stress-Induced Premature Senescence in Wharton's Jelly-Derived Mesenchymal Stem Cells

Choo¹,

Tai²,

Hymavathee³

et al. 2014

Int. J. Med. Sci.

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Background: On in vitro expansion for therapeutic purposes, the regenerative potentials of mesenchymal stem cells (MSCs) decline and rapidly enter pre-mature senescence probably involving oxidative stress. To develop strategies to prevent or slow down the decline of regenerative potentials in MSC culture, it is important to first address damages caused by oxidative stress-induced premature senescence (OSIPS). However, most existing OSIPS study models involve either long-term culture to achieve growth arrest or immediate growth arrest post oxidative agent treatment and are unsuitable for post-induction studies. Methods: In this work, we aimed to establish an OSIPS model of MSCs derived from Wharton's Jelly by hydrogen peroxide (H2O2) treatment. Results: The optimal H2O2 concentration was determined to be 200 µM to achieve OSIPS when MSC reached growth arrest in 3 to 4 passages post-H2O2 treatment. H2O2-treated cells became heterogeneous in morphology, and were irregularly enlarged and flattened with granular cytoplasm. The cells were stained positive for SA-β-galactosidase, a senescence marker, and were shown to express elevated levels of other well-characterized senescence molecular markers, including p53, p21, p16 and lysosomal β-galactosidase (GLB1) in real-time RT-PCR analysis. The OSIPS-like features were confirmed with three independent WJ-MSC lines. Conclusion: The establishment of an OSIPS model of WJ-MSC is a first step for subsequent investigation on molecular mechanisms of senescence and for screening potential anti-oxidative agents to delay or revert stressed-induced senescence.

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Section: Resultssupporting

confidence: 92%

Oxidative Stress-Induced Premature Senescence in Wharton's Jelly-Derived Mesenchymal Stem Cells

Choo¹,

Tai²,

Hymavathee³

et al. 2014

Int. J. Med. Sci.

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“…Similarly, when a PEG-based hydrogel was functionalized with the laminin-derived adhesion peptide YIGSR, the resulting biomaterial significantly enhanced intracellular triglyceride accumulation in encapsulated adipocytes and ultimately promoted the formation of coherent adipose tissue-like structures featuring many mature unilocular fat cells [361]. Although MSCs have been suggested to be a potential source for disc tissue regeneration, these cells neither differentiate into NP-like cells nor regenerate matrix with unique characteristics matching that of immature NP tissues of the IVD unless co-cultured with human NP cells or placed in a laminin-rich culture environment [362,363]. Indeed, substantial evidence suggests that NP cell-laminin interplay is unique to the immature disc because immature NP cells were found to express specific laminin isoforms and laminin-binding receptors [364,365].…”

Section: Human Tissue Ecm-based Biomaterialsmentioning

confidence: 99%

Advancing biomaterials of human origin for tissue engineering

Chen

Liu

2016

Progress in Polymer Science

925

513

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Biomaterials have played an increasingly prominent role in the success of biomedical devices and in the development of tissue engineering, which seeks to unlock the regenerative potential innate to human tissues/organs in a state of deterioration and to restore or reestablish normal bodily function. Advances in our understanding of regenerative biomaterials and their roles in new tissue formation can potentially open a new frontier in the fast-growing field of regenerative medicine. Taking inspiration from the role and multi-component construction of native extracellular matrices (ECMs) for cell accommodation, the synthetic biomaterials produced today routinely incorporate biologically active components to define an artificial in vivo milieu with complex and dynamic interactions that foster and regulate stem cells, similar to the events occurring in a natural cellular microenvironment. The range and degree of biomaterial sophistication have also dramatically increased as more knowledge has accumulated through materials science, matrix biology and tissue engineering. However, achieving clinical translation and commercial success requires regenerative biomaterials to be not only efficacious and safe but also cost-effective and convenient for use and production. Utilizing biomaterials of human origin as building blocks for therapeutic purposes has provided a facilitated approach that closely mimics the critical aspects of natural tissue with regard to its physical and chemical properties for the orchestration of wound healing and tissue regeneration. In addition to directly using tissue transfers and transplants for repair, new applications of human-derived biomaterials are now focusing on the use of naturally occurring biomacromolecules, decellularized ECM scaffolds and autologous preparations rich in growth factors/non-expanded stem cells to either target acceleration/magnification of the body's own repair capacity or use nature's paradigms to create new tissues for restoration. In particular, there is increasing interest in separating ECMs into simplified functional domains and/or biopolymeric assemblies so that these components/constituents can be discretely exploited and manipulated for the production of bioscaffolds and new biomimetic biomaterials. Here, following an overview of tissue auto-/allo-transplantation, we discuss the recent trends and advances as well as the challenges and future directions in the evolution and application of human-derived biomaterials for reconstructive surgery and tissue engineering. In particular, we focus on an exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications and to provide inspiration for the design of future biomaterials.

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“…[9][10][11] Over the past few years, many biological approaches have been used to promote the biological activity of NPCs and to stimulate the regeneration of the IVD, such as the injection of growth factors, [12][13][14] biomaterials, [15][16][17] and cells. [18][19][20] Although tremendous progress has been accomplished, therapeutic limits still exist when these approaches are used alone. The growth factors always degenerate too quickly to result in long-time bioactivity.…”

Section: Introductionmentioning

confidence: 99%

Biological Evaluation of Human Degenerated Nucleus Pulposus Cells in Functionalized Self-Assembling Peptide Nanofiber Hydrogel Scaffold

Tao

Zhang

Wang

et al. 2014

Tissue Engineering Part A

Self Cite

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Nucleus pulposus (NP) tissue engineering has been proposed as a novel biological treatment for early-stage intervertebral disc degeneration. In this study, a novel functional self-assembling peptide PKP was first designed by linking the short functional motif of bone morphogenetic protein-7 (BMP7) to the C-terminal of RADA16-I, and another new functional self-assembling peptide was obtained by mixing RKP with RADA16-I. Then, the biocompatibilities and bioactivities of RKP and RAD-RKP for human degenerated nucleus pulposus cells (hNPCs) were studied in vitro. Atomic force microscopy and scanning electron microscopy (SEM) confirmed that both RKP and RAD-RKP could self-assemble into three-dimensional (3D) nanofiber hydrogel scaffolds in a culture medium at 37°C. After the hNPCs were cultured in 3D scaffolds, both RKP and RAD-RKP exhibited reliable attachment and extremely low cytotoxicities (<14%), which were verified by SEM and cytotoxity assays, respectively. Our results also showed that the functional-based scaffolds could increase the proliferation and migration of hNPCs after 7 days compared with culture plates and pure RADA16-I. Quantitative real-time polymerase chain reaction demonstrated that the expressions of collagen II α1, Sox-9, and aggrecan were upregulated, while collagen I α1 was downregulated by functional-based scaffolds after 28 days. Furthermore, we also confirmed that RAD-RKP exhibited a higher hNPC proliferation, migration, and expression of Sox-9 and aggrecan compared with pure RKP. Therefore, the results of this study indicated that the BMP7 short motif-designed functional self-assembling peptide nanofiber hydrogels could be used as excellent scaffolds in NP tissue engineering, and RAD-RKP might have further potential application in human mild degenerated NP tissue regeneration.

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Differentiation of Human Wharton's Jelly Cells Toward Nucleus Pulposus-Like Cells After Coculture with Nucleus Pulposus Cells In Vitro

Cited by 31 publications

References 43 publications

Oxidative Stress-Induced Premature Senescence in Wharton's Jelly-Derived Mesenchymal Stem Cells

Oxidative Stress-Induced Premature Senescence in Wharton's Jelly-Derived Mesenchymal Stem Cells

Advancing biomaterials of human origin for tissue engineering

Biological Evaluation of Human Degenerated Nucleus Pulposus Cells in Functionalized Self-Assembling Peptide Nanofiber Hydrogel Scaffold

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