Differentiation of Human Umbilical Cord Matrix Mesenchymal Stem Cells into Neural-Like Progenitor Cells and Maturation into an Oligodendroglial-Like Lineage

Abstract

Mesenchymal stem cells (MSCs) are viewed as safe, readily available and promising adult stem cells, which are currently used in several clinical trials. Additionally, their soluble-factor secretion and multi-lineage differentiation capacities place MSCs in the forefront of stem cell types with expected near-future clinical applications. In the present work MSCs were isolated from the umbilical cord matrix (Wharton's jelly) of human umbilical cord samples. The cells were thoroughly characterized and confirmed as bona-fide MSCs, presenting in vitro low generation time, high proliferative and colony-forming unit-fibroblast (CFU-F) capacity, typical MSC immunophenotype and osteogenic, chondrogenic and adipogenic differentiation capacity. The cells were additionally subjected to an oligodendroglial-oriented step-wise differentiation protocol in order to test their neural- and oligodendroglial-like differentiation capacity. The results confirmed the neural-like plasticity of MSCs, and suggested that the cells presented an oligodendroglial-like phenotype throughout the differentiation protocol, in several aspects sharing characteristics common to those of bona-fide oligodendrocyte precursor cells and differentiated oligodendrocytes.

Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

“…hUCM-MSCs were generously provided by Anhui Huien Biotechnology Corporation, LTD. (Hefei, Anhui, China), and they have been used in previous studies [54, 55]. To isolate the hUCM-MSCs, UCMs from the UCM of consenting patients were enzymatically dissociated in Dulbecco’s modified Eagle medium (DMEM) with 0.25% (w/v) trypsin (Gibco, Carlsbad, CA, USA), 300 U collagenase type II (Gibco), and 10% (v/v) fetal bovine serum (FBS, Gibco).…”

EID3 directly associates with DNMT3A during transdifferentiation of human umbilical cord mesenchymal stem cells to NPC-like cells

“…The question of how to quickly obtain a large number of NSCs is a significant issue in regenerative medicine. Fortunately, MSCs offer a possible source of NSCs, since they are relatively abundant, are easy to isolate, proliferate quickly, and reports indicate that they can be induced to transdifferentiate to a large number NSC-like cells in a short period424. However, there has been controversy over whether MSCs can naturally transdifferentiate to NSCLs and not just through induction with exogenous transcription factors.…”

Mesenchymal Stem Cells: An Innovative Approach in Pharmacokinetics

“…The studies impressively demonstrate that capacity of neuronal precursor cells prepared from self-assemble 3D "organoids" and hMSCs offer a consistent, scalable source for diverse neural cell types including neuron, glial cells, and astrocytes in the presence of appropriate inducers. Recent report suggests that hUCMSCs differentiate into neural-like progenitor cells and matured oligodendroglial-like lineage when cultured in neurobasal medium supplemented with B27, 10 ng/ml of human recombinant bFGF, platelet-derived growth factor-AA and 100 ng/ml of Sonic Hedgehog [55,56]. Toxic effects of chemical compounds, environmental factors, naturally occurring substances, anthropogenic agents, and EDs can lead to neurotoxicity.…”