Differentiation of Human Pluripotent Stem Cells into Mesodermal and Ectodermal Derivatives Is Independent of the Type of Isogenic Reprogrammed Somatic Cells

Philonenko, Elena S.; Shutova, Maria V.; Khomyakova, E. A.; Vassina, Ekaterina M.; Lebedeva, Olga; Киселев, С. Л.; Lagarkova, M. A.

doi:10.32607/20758251-2017-9-1-68-74

Cited by 5 publications

(5 citation statements)

References 20 publications

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“…Currently, multiple strategies have been developed for spontaneous and directed differentiation of hPSCs into specialized cells from all three germ layers [98][99][100]. In the case of mesoderm, recent advances have demonstrated the opportunity to derive various types of mesenchymal cell populations from hPSCs.…”

Section: Isogenic Cellular Models Of Different Stages Of Human Develo...mentioning

confidence: 99%

Mesodermal Derivatives of Pluripotent Stem Cells Route to Scarless Healing

Suzdaltseva

Kiselev

2023

IJMS

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Scar formation during normal tissue regeneration in adults may result in noticeable cosmetic and functional defects and have a significant impact on the quality of life. In contrast, fetal tissues in the mid-gestation period are known to be capable of complete regeneration with the restitution of the initial architecture, organization, and functional activity. Successful treatments that are targeted to minimize scarring can be realized by understanding the cellular and molecular mechanisms of fetal wound regeneration. However, such experiments are limited by the inaccessibility of fetal material for comparable studies. For this reason, the molecular mechanisms of fetal regeneration remain unknown. Mesenchymal stromal cells (MSCs) are central to tissue repair because the molecules they secrete are involved in the regulation of inflammation, angiogenesis, and remodeling of the extracellular matrix. The mesodermal differentiation of human pluripotent stem cells (hPSCs) recapitulates the sequential steps of embryogenesis in vitro and provides the opportunity to generate the isogenic cell models of MSCs corresponding to different stages of human development. Further investigation of the functional activity of cells from stromal differon in a pro-inflammatory microenvironment will procure the molecular tools to better understand the fundamental mechanisms of fetal tissue regeneration. Herein, we review recent advances in the generation of clonal precursors of primitive mesoderm cells and MSCs from hPSCs and discuss critical factors that determine the functional activity of MSCs-like cells in a pro-inflammatory microenvironment in order to identify therapeutic targets for minimizing scarring.

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Section: Isogenic Cellular Models Of Different Stages Of Human Develo...mentioning

confidence: 99%

Mesodermal Derivatives of Pluripotent Stem Cells Route to Scarless Healing

Suzdaltseva

Kiselev

2023

IJMS

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“…Differences among hPSC lines mainly include DNA methylation[9-13] and gene expression[1,8], which have functional implications for both ESC and iPSC lines[1]. Particularly, for iPSCs, the variations may be donor-dependent[14,15] or original cell type-dependent[9], while, in other studies, this relationship was not found[10,16]. Moreover, the characteristics of hPSCs may differ depending on the number of passages[17], culture medium components[18], and feeder conditions[19].…”

Section: Introductionmentioning

confidence: 99%

Predicting differentiation potential of human pluripotent stem cells: Possibilities and challenges

Liu¹,

Zheng²

2019

WJSC

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The capability of human pluripotent stem cell (hPSC) lines to propagate indefinitely and differentiate into derivatives of three embryonic germ layers makes these cells be powerful tools for basic scientific research and promising agents for translational medicine. However, variations in differentiation tendency and efficiency as well as pluripotency maintenance necessitate the selection of hPSC lines for the intended applications to save time and cost. To screen the qualified cell lines and exclude problematic cell lines, their pluripotency must be confirmed initially by traditional methods such as teratoma formation or by high-throughput gene expression profiling assay. Additionally, their differentiation potential, particularly the lineage-specific differentiation propensities of hPSC lines, should be predicted in an early stage. As a complement to the teratoma assay, RNA sequencing data provide a quantitative estimate of the differentiation ability of hPSCs in vivo . Moreover, multiple scorecards have been developed based on selected gene sets for predicting the differentiation potential into three germ layers or the desired cell type many days before terminal differentiation. For clinical application of hPSCs, the malignant potential of the cells must also be evaluated. A combination of histologic examination of teratoma with quantitation of gene expression data derived from teratoma tissue provides safety-related predictive information by detecting immature teratomas, malignancy marker expression, and other parameters. Although various prediction methods are available, distinct limitations remain such as the discordance of results between different assays and requirement of a long time and high labor and cost, restricting their wide applications in routine studies. Therefore, simpler and more rapid detection assays with high specificity and sensitivity that can be used to monitor the status of hPSCs at any time and fewer targeted markers that are more specific for a given desired cell type are urgently needed.

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“…During differentiation, it is expected that stem cells lose the expression of the pluripotency-related genes OCT4 and NANOG and begin to express markers associated with differentiation, such as GATA4 and GATA6 (Miyamoto et al, 2015). An important issue regarding epigenetic markers in stem cells is that the current methods of iPSC cultivation allow the maintenance of the epigenetic profile over a long period (Philonenko et al, 2017). Such epigenetic control may be also mediated by miRNAs ( Fig.…”

Section: Introductionmentioning

confidence: 99%

“…Besides the ability to obtain embryonic bodies from iPSCs, another advantage in using iPSCs for in vitro cell differentiation is that technical manipulations, such as selection of clones or cell sorting, do not interfere with their ability to differentiate into hematopoietic tissue (Philonenko et al, 2017). Even if iPSCs require genetic manipulations (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Hematopoietic stem cells from induced pluripotent stem cells – considering the role of microRNA as a cell differentiation regulator

Ferreira

Calin

Picanço‐Castro

et al. 2018

Journal of Cell Science

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Although hematopoietic stem cell (HSC) therapy for hematological diseases can lead to a good outcome from the clinical point of view, the limited number of ideal donors, the comorbidity of patients and the increasing number of elderly patients may limit the application of this therapy. HSCs can be generated from induced pluripotent stem cells (iPSCs), which requires the understanding of the bone marrow and liver niches components and function iPSCs have been extensively applied in several studies involving disease models, drug screening and cellular replacement therapies. However, the somatic reprogramming by transcription factors is a low-efficiency process. Moreover, the reprogramming process is also regulated by microRNAs (miRNAs), which modulate the expression of the transcription factors OCT-4 (also known as POU5F1), SOX-2, KLF-4 and MYC, leading somatic cells to a pluripotent state. In this Review, we present an overview of the challenges of cell reprogramming protocols with regard to HSC generation from iPSCs, and highlight the potential role of miRNAs in cell reprogramming and in the differentiation of induced pluripotent stem cells.

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Differentiation of Human Pluripotent Stem Cells into Mesodermal and Ectodermal Derivatives Is Independent of the Type of Isogenic Reprogrammed Somatic Cells

Cited by 5 publications

References 20 publications

Mesodermal Derivatives of Pluripotent Stem Cells Route to Scarless Healing

Mesodermal Derivatives of Pluripotent Stem Cells Route to Scarless Healing

Predicting differentiation potential of human pluripotent stem cells: Possibilities and challenges

Hematopoietic stem cells from induced pluripotent stem cells – considering the role of microRNA as a cell differentiation regulator

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