Differentiation of Human Induced Pluripotent Stem Cells into Cortical Neurons to Advance Precision Medicine

Silva, M. Catarina; Nandi, Ghata; Haggarty, Stephen J.

doi:10.1007/978-1-0716-1979-7_10

Cited by 2 publications

(1 citation statement)

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“…Due to the recent progress in the field, it is now possible to identify programming factors in a more effective way through the use of several techniques, such as single-cell RNA sequencing (sc-RNAseq), in multiple steps of development and trajectory inference. Currently, there are already several protocols available to differentiate hIPSCs into almost all neuronal cell types, such as motor [178][179][180][181][182], striatal [183], cortical [184][185][186] excitatory [187] and GABAergic [188], dopaminergic [189][190][191][192][193] and serotoninergic [194,195], and peripheral sensory neurons [196,197].…”

Section: Hipscs Differentiation Into Neuronal Populationsmentioning

confidence: 99%

Transition from Animal-Based to Human Induced Pluripotent Stem Cells (iPSCs)-Based Models of Neurodevelopmental Disorders: Opportunities and Challenges

Guerreiro

Maciel

2023

Cells

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Neurodevelopmental disorders (NDDs) arise from the disruption of highly coordinated mechanisms underlying brain development, which results in impaired sensory, motor and/or cognitive functions. Although rodent models have offered very relevant insights to the field, the translation of findings to clinics, particularly regarding therapeutic approaches for these diseases, remains challenging. Part of the explanation for this failure may be the genetic differences—some targets not being conserved between species—and, most importantly, the differences in regulation of gene expression. This prompts the use of human-derived models to study NDDS. The generation of human induced pluripotent stem cells (hIPSCs) added a new suitable alternative to overcome species limitations, allowing for the study of human neuronal development while maintaining the genetic background of the donor patient. Several hIPSC models of NDDs already proved their worth by mimicking several pathological phenotypes found in humans. In this review, we highlight the utility of hIPSCs to pave new paths for NDD research and development of new therapeutic tools, summarize the challenges and advances of hIPSC-culture and neuronal differentiation protocols and discuss the best way to take advantage of these models, illustrating this with examples of success for some NDDs.

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